LOCUS       sequence-30176-e         656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR
  TITLE     Constitutive Gs activation using a single-construct 
            tetracycline-inducible expression system in embryonic stem cells and
            mice.
  JOURNAL   Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
  PUBMED    21375737
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res Ther. 2011 Mar 4. 2(2):11."
COMMENT     Sequence Label: pEnt R3L2 TetO(sh) mCh-Rs1-2
FEATURES             Location/Qualifiers
     source          1..656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..135
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagacctgg
       61 tttctcgagg gcagcggcgc gaccaacttt agcctgctga aacaggcggg cgatgtggaa
      121 gaaaacccag gaccgaccgg tatcaagacg atcatcgccc tgagctacat cttctgcctg
      181 gtattcgccg actacaagga cgatgatgac gccagcggcc gcgcccgggc ggatcccccg
      241 ggctgcagga tggacaaact tgatgctaat gtgagttctg aggagggttt cgggtcagtg
      301 gagaaggtgg tgctgctcac gtttctctcg acggttatcc tgatggccat cttggggaac
      361 ctgctggtga tggtggctgt gtgctgggac aggcagctca ggaaaataaa aacaaattat
      421 ttcattgtat ctcttgcttt tgcggatctg ctggtttcgg tgctggtgat gccctttggt
      481 gccattgagc tggttcaaga catctggatt tatggggagg tgttttgtct tgttcggaca
      541 tctctggccg tcctgctcac aacggcatcg atttttcacc tgtgctgcat ttctctggat
      601 aggtattacg ccatctgctg nccagcnntt tgtgtttaaa gagaaaacaa anaana
//
