LOCUS       sequence-30182-e         630 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR
  TITLE     Constitutive Gs activation using a single-construct 
            tetracycline-inducible expression system in embryonic stem cells and
            mice.
  JOURNAL   Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
  PUBMED    21375737
REFERENCE   2  (bases 1 to 630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res Ther. 2011 Mar 4. 2(2):11."
COMMENT     Sequence Label: Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2
FEATURES             Location/Qualifiers
     source          1..630
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             256..312
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ggcgccctgn agggcgagat caagcagagg ctgaagctga agganggcgg ccactacgac
       61 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
      121 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
      181 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gacctggttt
      241 ctcgagggca gcggcgcgac caactttagc ctgctgaaac aggcgggcga tgtggaagaa
      301 aacccaggac cgaccggtat caagacgatc atcgccctga gctacatctt ctgcctggta
      361 ttcgccgact acaaggacga tgatgacgcc agcggccgcg cccgggcgga tcccccgggc
      421 tgcaggatgg acaaacttga tgctaatgtg agttctgagg agggtttcgg gtcagtggag
      481 aaggtggtgc tgctcacgtt tctctcgacg gttatcctga tggccatctt ggggaacctg
      541 ctggtgatgg tggctgtgtg ctgggacagg cagctcagga aaataaaaac aaattatttc
      601 attgtatctc ttgcttttgc ggatctgctg
//
