LOCUS       sequence-30184-e         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Griffis ER, Stuurman N, Vale RD
  TITLE     Spindly, a novel protein essential for silencing the spindle 
            assembly checkpoint, recruits dynein to the kinetochore.
  JOURNAL   J Cell Biol. 2007 Jun 18. 177(6):1005-15.
  PUBMED    17576797
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2007 Jun 18. 177(6):1005-15."
COMMENT     Sequence Label: pMT-GFP-Spindly
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..66)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    92..116
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtgaggtga tgatgaccgg tacgcgtaga atcgagaccg aggagagggt tagggatagg
       61 cttaccttcg aagggccctc tagcttacgt caccactttg tacaagaaag ctgggtcggc
      121 gcgcccaccc tttttcataa ttggtttacg agagctcaca acaatacgcc gtatgatgtt
      181 tggcttctta attgtgggtt ttacgtcctg gttgtcttca ctttcttttg gcttgtcatt
      241 ttccttggtg gtttccccat ttggacatct ctcaagcgga cttaaattgt gaacaatggg
      301 atcgtttgcg ctaaacttca cagccttttg cgaatcttcg gcgaagactt gccgtttttt
      361 gctgaggatg gagcggatat tttcgttggt cgagtctttg atgacgggaa tcctcacagg
      421 ggttcctttg cgctgcggtt tttgtggcgt tggactgtcc agaatttcca cactcaatgt
      481 cccatcttgc ttgatgggag attccttggc cgcctcatcg ctgctgtctt cagtttccgt
      541 ttccagcttt atgagcttca caggagttcc ttttcgctgc ggtttagcag gcgttactgt
      601 accaggcagt cttgctccca gcagttcttc gttggacttc acctttatcc ctcccagtcg
      661 ctgcttaact ggtgtaccct tcatcaatgg ggtgggctgg gcgttccacg gaat
//
