LOCUS       sequence-30185-e         613 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Griffis ER, Stuurman N, Vale RD
  TITLE     Spindly, a novel protein essential for silencing the spindle 
            assembly checkpoint, recruits dynein to the kinetochore.
  JOURNAL   J Cell Biol. 2007 Jun 18. 177(6):1005-15.
  PUBMED    17576797
REFERENCE   2  (bases 1 to 613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2007 Jun 18. 177(6):1005-15."
COMMENT     Sequence Label: pMT-GFP-Spindly
FEATURES             Location/Qualifiers
     source          1..613
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      57..66
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(63..65,66..68,69..581)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(108..129)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(369..388)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctactagtcc agtgtggtgg aattctgcag atatcgaggc ctgtctagag aagcccgcca
       61 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      121 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      181 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      241 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
      301 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      361 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      421 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
      481 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
      541 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
      601 ccgaccacta cca
//
