LOCUS       sequence-30187-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Griffis ER, Stuurman N, Vale RD
  TITLE     Spindly, a novel protein essential for silencing the spindle 
            assembly checkpoint, recruits dynein to the kinetochore.
  JOURNAL   J Cell Biol. 2007 Jun 18. 177(6):1005-15.
  PUBMED    17576797
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2007 Jun 18. 177(6):1005-15."
COMMENT     Sequence Label: pMT-GFP-p150Dynactin
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..73)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    99..123
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gttanatggt gaggtgatga tgaccggtac gcgtagaatc gagaccgagg agagggttag
       61 ggataggctt accttcgaag ggccctctag cttacgtcac cactttgtac aagaaagctg
      121 ggtcggcgcg cccacccttg atctgcagca cgcgcttcac atcgacggtg ggaaaggagg
      181 cgaactgtcc gtgagttgca cgatggggtt tcctttgcag atgctccgtc aggatgtcgg
      241 aggccagctg ggaagccttc gagctgatct cgcccttgag tggaactggc tggttgcgcc
      301 tttggagtgc cacggcgtcg atacgtgtac cggaattcac tgaatcctgc gagcgcacct
      361 gcagcagcga caatacccag gcgtgcttca tcctggtcag ctcggattcc aaagcggtta
      421 tgcgctgatc ctgtggctga ggcacatgca ggggctcaaa ctgggacaac ttggcacgca
      481 tatcctgtgc ctgcagacgc agtcgttggt tgcgctcctg gttgaaggcg ttcttcagca
      541 actccacttc ctcggccacc actggagcat gcggagacaa tagaanttgc tgagccggac
      601 atggactgtg ctgaacgant atgtgcggga tggatncnat atattgtgcn ggnagtggga
      661 tcccanccgg cttnaccttg cctgtaatcc agtacnccag ttcagcttca gcttgtcgcg
      721 tagatcgctc ttctcgctct cnagcgcgtc gatatcgctt tgcaggtggt ccatcgtctc
      781 ctcaaactcc ttctcntaan ctgcagctgc aangagnttc ctcgtactct gctccattgt
      841 cnacgcgtgc tcgtac
//
