LOCUS       sequence-30193-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Vale RD, Spudich JA, Griffis ER
  TITLE     Dynamics of myosin, microtubules, and Kinesin-6 at the cortex during
            cytokinesis in Drosophila S2 cells.
  JOURNAL   J Cell Biol. 2009 Sep 7. 186(5):727-38.
  PUBMED    19720876
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2009 Sep 7. 186(5):727-38."
COMMENT     Sequence Label: pMT-Pav-GFP
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..55)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(join(72..785,786..788,789..791))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGAVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKND
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(117..138)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     725..746
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gatgaccggt acgcgtagaa tcgagaccga ggagagggtt agggataggc ttaccttcga
       61 accgcgggcc cttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
      121 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg
      181 gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
      241 tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc
      301 accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg
      361 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
      421 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
      481 tcgttcttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag
      541 aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg
      601 gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg
      661 gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg
      721 tttacgtcgc cgtccagctc gaccaggatg ggcaccgccc cggtgaacag ctcctcgccc
      781 ttgctcacca tgcggccgca tttcgacttc ttgctgctgt gtccctcaat gccgagattg
      841 cagcgggagg ctacgtcctg ggctgaggtg accgtgctgg gcactgcacc gccgccca
//
