LOCUS       sequence-301968-        6717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse gamma-synuclein (mSncg) promoter-mediates gene expression in 
            retinal ganglion cells with fluorescent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Wang Q, Zhuang PS, Huang H, Li L, Liu L, Webber HC, Dalal R, Siew L,
            Fligor CM, Chang KC, Nahmou M, Kreymerman A, Sun Y, Meyer JS, 
            Goldberg JL, Hu Y
  TITLE     Mouse gamma-Synuclein Promoter-Mediated Gene Expression and Editing 
            in Mammalian Retinal Ganglion Cells.
  JOURNAL   J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020.
  PUBMED    32300046
REFERENCE   2  (bases 1 to 6717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020."
COMMENT     Sequence Label: pAM-AAV-mSncg-EGFP
FEATURES             Location/Qualifiers
     source          1..6717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        83..101
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     83..100
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     repeat_region   122..251
                     /label=AAV2 ITR
     CDS             join(1881..1883,1884..1886,1887..2600)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1881..1883,1884..1886,1887..2597)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1926..1947)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2187..2206)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2534..2555
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2658..3246
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2711..2731)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3129..3140)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3273..3290)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3279..3503
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(3713..4042)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3727..3862
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4027..4047
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(4288..4876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4368..4387)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5047..5838,5839..5907))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5670..5689
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5908..6012)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6080..6098
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6136..6158)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6258..6277
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6491..6510
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atgctacaca attaggcttg tacatattgt cgttagaacg cggctacaat taatacataa
       61 ccttatgtat catacacata cgatttaggt gacactatag aatacacgga attaattcta
      121 gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt
      181 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
      241 taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt agccatgctc
      301 taggtaccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      361 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      421 tatcatatgg tcccatgcca ctagtgggag ctgtgttacc tgttgcagcc ccacccaaag
      481 cccctgctat aggtcaagca ggaatcaccc tgccatcccc agcctggggc ctggagtacc
      541 agatccagga aactagcatc ccttagctat agagatagcc acacatcagc ccattcctca
      601 gatgtgtatc tggggctcag acatcatctc ccgatctccg acaagggcag gatttcctta
      661 ccgtctgatg gggtctctgc tggtatcctc agcccctagt ctccagcctt caggcatgcc
      721 gggcctattg agatgggaga acttggtacc ggggtcctgt gcccaggacc ctagcagtcc
      781 ccagctcagg tacaccccaa agcccagcag cagtgtcggg atcatggtga ggggctcctg
      841 tgctgatgct cagccttaca agtgactctc aaatttgctg gtgatgtggt cttcaagcga
      901 aatgtcagaa agaaaagaaa acacgaggac aacaaaggga ggaagtggcc tggtccggcc
      961 cacccggcaa gtctcatccg cccccgcccc cgccccttcc agcctggccc ccttggaggc
     1021 ctccaaccac tcaggtcaat tcctgtgtcc tgagggcact tgaatcaggg acacgggatt
     1081 tggtagacac ataaaggtgg ccccattaaa cttatttctc caggactctg tcgtgggcct
     1141 ggaggagatc tggtgccacc catactgttg gccaggaagt ggggaacggg cacatcacac
     1201 ctgctcggca ccttgggcta tgggaactag caggtgggtg ggaactcaga gaaggaaagg
     1261 gactatgcta gaatcacaca gcgggcagcc cagtctaggg catggggagc agctttgggt
     1321 gtttctggcc tcagccttcc aacaggtttg gctagagctc caggctcaag agcatccagg
     1381 atacagtggg gaactggata acagggcagc ctgcaggttg gccattcatt ggattggccc
     1441 tgaccccggc ccagcctggg acactgaggc atcatcagtc aaggcacttt tcttctgcat
     1501 ataagagcca gggcacgaga ccaccagggc tttccaagga tgaatgaggt gtaatgatag
     1561 attaggatat gtccagcctc caacacgctc tccctccccc agggccaaca agagtcagca
     1621 gggcagaata gagccagtag gggcccgggc cctgctcgct ggtatccccg tgaggcatgc
     1681 cttctctctg gcccgccctc cctgccccca ccctggcccg ggctggctgg gctccagcca
     1741 gcagccacag catcaatatt tcatctgcgt caataagagg cagtagcagc agagacagcg
     1801 gctgcggcag cactccagtc catagcttgc agcagccagg ttccatcctt gcaaacacca
     1861 tggacgtctt caaggaattc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     1921 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     1981 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     2041 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     2101 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     2161 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     2221 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2281 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     2341 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     2401 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     2461 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     2521 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     2581 tggacgagct gtacaagtaa agatctacgc gtggtacctc tagagtcgac ccgggcggcc
     2641 tcgagaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     2701 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     2761 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     2821 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     2881 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     2941 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     3001 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     3061 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     3121 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     3181 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     3241 ccccgcatcg ataccgtcga ctcgctgatc agcctcgact gtgccttcta gttgccagcc
     3301 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     3361 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     3421 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     3481 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgactag
     3541 agcatggcta cgtagataag tagcatggcg ggttaatcat taactacaag gaacccctag
     3601 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
     3661 aggtcgcccg acgcccgggc ggcctcagtg agcgagcgag cgcgcagagc tttttgcaaa
     3721 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg
     3781 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
     3841 tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat
     3901 tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg
     3961 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
     4021 ttccacaccc taactgacac acattccaca gctgcattaa tgaatcggcc aacgcgcggg
     4081 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     4141 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4201 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4261 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4321 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4381 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4441 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4501 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4561 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4621 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4681 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     4741 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4801 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     4861 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     4921 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     4981 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     5041 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     5101 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     5161 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     5221 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     5281 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     5341 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     5401 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     5461 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     5521 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     5581 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     5641 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     5701 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     5761 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     5821 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     5881 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     5941 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     6001 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     6061 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     6121 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     6181 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     6241 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc attcgacgct
     6301 ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca
     6361 ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg
     6421 ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat
     6481 cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga
     6541 tgccggccac gatgcgtccg gcgtagagga tctggctagc gatgaccctg ctgattggtt
     6601 cgctgaccat ttccgggtgc gggacggcgt taccagaaac tcagaaggtt cgtccaacca
     6661 aaccgactct gacggcagtt tacgagagag atgatagggt ctgcttcagt aagccag
//
