LOCUS       sequence-301973-        6292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Truncated mouse gamma-synuclein (mSncg-1.03kb) promoter-mediates 
            gene expression in mammalian retinal ganglion cells with fluorescent
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Wang Q, Zhuang PS, Huang H, Li L, Liu L, Webber HC, Dalal R, Siew L,
            Fligor CM, Chang KC, Nahmou M, Kreymerman A, Sun Y, Meyer JS, 
            Goldberg JL, Hu Y
  TITLE     Mouse gamma-Synuclein Promoter-Mediated Gene Expression and Editing 
            in Mammalian Retinal Ganglion Cells.
  JOURNAL   J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020.
  PUBMED    32300046
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020."
COMMENT     Sequence Label: pAM-AAV-mSncg-1.03kb-EGFP
FEATURES             Location/Qualifiers
     source          1..6292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        17..35
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     17..34
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     repeat_region   56..185
                     /label=AAV2 ITR
     CDS             join(1390..1392,1393..1395,1396..2109)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1390..1392,1393..1395,1396..2106)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1435..1456)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1696..1715)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2043..2064
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2167..2755
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2220..2240)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2638..2649)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2782..2799)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2788..3012
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(3222..3551)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3236..3371
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3536..3556
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(3797..4385)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3877..3896)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4556..5347,5348..5416))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5179..5198
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5417..5521)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5589..5607
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5645..5667)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5767..5786
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6000..6019
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gtatcataca catacgattt aggtgacact atagaataca cggaattaat tctagctgcg
       61 cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg
      121 cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg
      181 ttccttgtag ttaatgatta acccgccatg ctacttatct acgtagccat gctctaggta
      241 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      301 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      361 agccttacaa gtgactctca aatttgctgg tgatgtggtc ttcaagcgaa atgtcagaaa
      421 gaaaagaaaa cacgaggaca acaaagggag gaagtggcct ggtccggccc acccggcaag
      481 tctcatccgc ccccgccccc gccccttcca gcctggcccc cttggaggcc tccaaccact
      541 caggtcaatt cctgtgtcct gagggcactt gaatcaggga cacgggattt ggtagacaca
      601 taaaggtggc cccattaaac ttatttctcc aggactctgt cgtgggcctg gaggagatct
      661 ggtgccaccc atactgttgg ccaggaagtg gggaacgggc acatcacacc tgctcggcac
      721 cttgggctat gggaactagc aggtgggtgg gaactcagag aaggaaaggg actatgctag
      781 aatcacacag cgggcagccc agtctagggc atggggagca gctttgggtg tttctggcct
      841 cagccttcca acaggtttgg ctagagctcc aggctcaaga gcatccagga tacagtgggg
      901 aactggataa cagggcagcc tgcaggttgg ccattcattg gattggccct gaccccggcc
      961 cagcctggga cactgaggca tcatcagtca aggcactttt cttctgcata taagagccag
     1021 ggcacgagac caccagggct ttccaaggat gaatgaggtg taatgataga ttaggatatg
     1081 tccagcctcc aacacgctct ccctccccca gggccaacaa gagtcagcag ggcagaatag
     1141 agccagtagg ggcccgggcc ctgctcgctg gtatccccgt gaggcatgcc ttctctctgg
     1201 cccgccctcc ctgcccccac cctggcccgg gctggctggg ctccagccag cagccacagc
     1261 atcaatattt catctgcgtc aataagaggc agtagcagca gagacagcgg ctgcggcagc
     1321 actccagtcc atagcttgca gcagccaggt tccatccttg caaacaccat ggacgtcttc
     1381 aaggaattca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1441 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     1501 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     1561 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     1621 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1681 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1741 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1801 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     1861 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     1921 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1981 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     2041 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     2101 tacaagtaaa gatctacgcg tggtacctct agagtcgacc cgggcggcct cgagaagctt
     2161 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     2221 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     2281 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     2341 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     2401 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     2461 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     2521 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     2581 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     2641 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     2701 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     2761 taccgtcgac tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     2821 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     2881 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     2941 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     3001 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctcgactaga gcatggctac
     3061 gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg
     3121 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     3181 cgcccgggcg gcctcagtga gcgagcgagc gcgcagagct ttttgcaaaa gcctaggcct
     3241 ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct
     3301 gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt
     3361 aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg
     3421 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa
     3481 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct
     3541 aactgacaca cattccacag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3601 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3661 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3721 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3781 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3841 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3901 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3961 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4021 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4081 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4141 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4201 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     4261 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4321 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4381 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4441 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4501 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     4561 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4621 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     4681 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     4741 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4801 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4861 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4921 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4981 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     5041 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     5101 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     5161 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     5221 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     5281 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     5341 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     5401 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     5461 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     5521 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     5581 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     5641 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     5701 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     5761 actatgcggc atcagagcag attgtactga gagtgcacca ttcgacgctc tcccttatgc
     5821 gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca
     5881 aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     5941 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     6001 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
     6061 atgcgtccgg cgtagaggat ctggctagcg atgaccctgc tgattggttc gctgaccatt
     6121 tccgggtgcg ggacggcgtt accagaaact cagaaggttc gtccaaccaa accgactctg
     6181 acggcagttt acgagagaga tgatagggtc tgcttcagta agccagatgc tacacaatta
     6241 ggcttgtaca tattgtcgtt agaacgcggc tacaattaat acataacctt at
//
