LOCUS       sequence-301975-        5929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Truncated mouse gamma-synuclein (mSncg-0.66kb) promoter-mediates 
            gene expression in retinal ganglion cells with fluorescent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Wang Q, Zhuang PS, Huang H, Li L, Liu L, Webber HC, Dalal R, Siew L,
            Fligor CM, Chang KC, Nahmou M, Kreymerman A, Sun Y, Meyer JS, 
            Goldberg JL, Hu Y
  TITLE     Mouse gamma-Synuclein Promoter-Mediated Gene Expression and Editing 
            in Mammalian Retinal Ganglion Cells.
  JOURNAL   J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020.
  PUBMED    32300046
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020."
COMMENT     Sequence Label: pAM-AAV-mSncg-0.66kb-EGFP
FEATURES             Location/Qualifiers
     source          1..5929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        183..201
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     183..200
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     repeat_region   222..351
                     /label=AAV2 ITR
     CDS             join(1193..1195,1196..1198,1199..1912)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1193..1195,1196..1198,1199..1909)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1238..1259)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1499..1518)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1846..1867
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1970..2558
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2023..2043)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2441..2452)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2585..2602)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2591..2815
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(3025..3354)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3039..3174
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3339..3359
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(3600..4188)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3680..3699)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4359..5150,5151..5219))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4982..5001
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5220..5324)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5392..5410
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5448..5470)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5570..5589
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5803..5822
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tgcgggacgg cgttaccaga aactcagaag gttcgtccaa ccaaaccgac tctgacggca
       61 gtttacgaga gagatgatag ggtctgcttc agtaagccag atgctacaca attaggcttg
      121 tacatattgt cgttagaacg cggctacaat taatacataa ccttatgtat catacacata
      181 cgatttaggt gacactatag aatacacgga attaattcta gctgcgcgct cgctcgctca
      241 ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga
      301 gcgagcgagc gcgcagagag ggagtggcca actccatcac taggggttcc ttgtagttaa
      361 tgattaaccc gccatgctac ttatctacgt agccatgctc taggtaccat tgacgtcaat
      421 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      481 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc tatgggaact
      541 agcaggtggg tgggaactca gagaaggaaa gggactatgc tagaatcaca cagcgggcag
      601 cccagtctag ggcatgggga gcagctttgg gtgtttctgg cctcagcctt ccaacaggtt
      661 tggctagagc tccaggctca agagcatcca ggatacagtg gggaactgga taacagggca
      721 gcctgcaggt tggccattca ttggattggc cctgaccccg gcccagcctg ggacactgag
      781 gcatcatcag tcaaggcact tttcttctgc atataagagc cagggcacga gaccaccagg
      841 gctttccaag gatgaatgag gtgtaatgat agattaggat atgtccagcc tccaacacgc
      901 tctccctccc ccagggccaa caagagtcag cagggcagaa tagagccagt aggggcccgg
      961 gccctgctcg ctggtatccc cgtgaggcat gccttctctc tggcccgccc tccctgcccc
     1021 caccctggcc cgggctggct gggctccagc cagcagccac agcatcaata tttcatctgc
     1081 gtcaataaga ggcagtagca gcagagacag cggctgcggc agcactccag tccatagctt
     1141 gcagcagcca ggttccatcc ttgcaaacac catggacgtc ttcaaggaat tcatggtgag
     1201 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     1261 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     1321 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     1381 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     1441 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     1501 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     1561 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     1621 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     1681 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     1741 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     1801 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     1861 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaagatctac
     1921 gcgtggtacc tctagagtcg acccgggcgg cctcgagaag cttatcgata atcaacctct
     1981 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     2041 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     2101 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     2161 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     2221 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     2281 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     2341 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     2401 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     2461 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     2521 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gactcgctga
     2581 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     2641 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     2701 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     2761 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     2821 gaggcggaaa gaaccagctg gggctcgact agagcatggc tacgtagata agtagcatgg
     2881 cgggttaatc attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg
     2941 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gcggcctcag
     3001 tgagcgagcg agcgcgcaga gctttttgca aaagcctagg cctccaaaaa agcctcctca
     3061 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat
     3121 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
     3181 agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc
     3241 tgctggggag cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg
     3301 catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca
     3361 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     3421 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3481 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3541 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3601 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3661 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3721 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3781 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3841 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3901 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3961 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4021 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4081 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4141 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4201 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4261 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     4321 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     4381 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     4441 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     4501 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     4561 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4621 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     4681 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     4741 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     4801 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     4861 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     4921 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     4981 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     5041 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     5101 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     5161 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     5221 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5281 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     5341 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     5401 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     5461 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     5521 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     5581 cagattgtac tgagagtgca ccattcgacg ctctccctta tgcgactcct gcattaggaa
     5641 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
     5701 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
     5761 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
     5821 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
     5881 gatctggcta gcgatgaccc tgctgattgg ttcgctgacc atttccggg
//
