LOCUS       sequence-301976-        5537 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Truncated mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates 
            gene expression in retinal ganglion cells with fluorescent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Wang Q, Zhuang PS, Huang H, Li L, Liu L, Webber HC, Dalal R, Siew L,
            Fligor CM, Chang KC, Nahmou M, Kreymerman A, Sun Y, Meyer JS, 
            Goldberg JL, Hu Y
  TITLE     Mouse gamma-Synuclein Promoter-Mediated Gene Expression and Editing 
            in Mammalian Retinal Ganglion Cells.
  JOURNAL   J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020.
  PUBMED    32300046
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020."
COMMENT     Sequence Label: pAM-AAV-mSncg-0.27kb-EGFP
FEATURES             Location/Qualifiers
     source          1..5537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        139..157
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     139..156
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     repeat_region   178..307
                     /label=AAV2 ITR
     CDS             join(757..759,760..762,763..1476)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(757..759,760..762,763..1473)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(802..823)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1063..1082)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1410..1431
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1534..2122
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1587..1607)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2005..2016)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2149..2166)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2155..2379
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(2589..2918)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2603..2738
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2903..2923
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(3164..3752)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3244..3263)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3923..4714,4715..4783))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4546..4565
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4784..4888)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4956..4974
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5012..5034)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5134..5153
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5367..5386
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 accgactctg acggcagttt acgagagaga tgatagggtc tgcttcagta agccagatgc
       61 tacacaatta ggcttgtaca tattgtcgtt agaacgcggc tacaattaat acataacctt
      121 atgtatcata cacatacgat ttaggtgaca ctatagaata cacggaatta attctagctg
      181 cgcgctcgct cgctcactga ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt
      241 cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc catcactagg
      301 ggttccttgt agttaatgat taacccgcca tgctacttat ctacgtagcc atgctctagg
      361 taccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      421 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      481 atatgaagag tcagcagggc agaatagagc cagtaggggc ccgggccctg ctcgctggta
      541 tccccgtgag gcatgccttc tctctggccc gccctccctg cccccaccct ggcccgggct
      601 ggctgggctc cagccagcag ccacagcatc aatatttcat ctgcgtcaat aagaggcagt
      661 agcagcagag acagcggctg cggcagcact ccagtccata gcttgcagca gccaggttcc
      721 atccttgcaa acaccatgga cgtcttcaag gaattcatgg tgagcaaggg cgaggagctg
      781 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      841 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      901 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
      961 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1021 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1081 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1141 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     1201 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     1261 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     1321 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     1381 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     1441 gggatcactc tcggcatgga cgagctgtac aagtaaagat ctacgcgtgg tacctctaga
     1501 gtcgacccgg gcggcctcga gaagcttatc gataatcaac ctctggatta caaaatttgt
     1561 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     1621 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     1681 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     1741 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     1801 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     1861 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     1921 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     1981 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     2041 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     2101 tccctttggg ccgcctcccc gcatcgatac cgtcgactcg ctgatcagcc tcgactgtgc
     2161 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     2221 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     2281 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     2341 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     2401 gctggggctc gactagagca tggctacgta gataagtagc atggcgggtt aatcattaac
     2461 tacaaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
     2521 gaggccgggc gaccaaaggt cgcccgacgc ccgggcggcc tcagtgagcg agcgagcgcg
     2581 cagagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc
     2641 tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc
     2701 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact
     2761 atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     2821 gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     2881 ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg cattaatgaa
     2941 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     3001 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     3061 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     3121 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3181 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3241 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3301 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3361 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3421 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3481 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3541 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3601 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3661 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3721 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3781 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3841 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3901 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3961 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     4021 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     4081 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     4141 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     4201 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     4261 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     4321 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     4381 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     4441 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     4501 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     4561 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     4621 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     4681 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     4741 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     4801 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4861 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     4921 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     4981 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     5041 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     5101 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     5161 tgcaccattc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
     5221 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
     5281 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
     5341 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
     5401 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatctg gctagcgatg
     5461 accctgctga ttggttcgct gaccatttcc gggtgcggga cggcgttacc agaaactcag
     5521 aaggttcgtc caaccaa
//
