LOCUS       sequence-301979-       12635 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector to introduce a constitutive rtta3G cassette and a 
            DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE 
            acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine 
            selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12635)
  AUTHORS   Nora EP, Caccianini L, Fudenberg G, So K, Kameswaran V, Nagle A, 
            Uebersohn A, Hajj B, Saux AL, Coulon A, Mirny LA, Pollard KS, Dahan 
            M, Bruneau BG
  TITLE     Molecular basis of CTCF binding polarity in genome folding.
  JOURNAL   Nat Commun. 2020 Nov 5;11(1):5612. doi: 10.1038/s41467-020-19283-x.
  PUBMED    33154377
REFERENCE   2  (bases 1 to 12635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-11-5"; volume: "11(1):5612. doi"; pages: " 
            10.1038/s41467-020-19283-x"
COMMENT     Sequence Label: pAU002 -
            pTRE3G-CTCF(DELTA1-265)-mRuby2-CAGGS-...
FEATURES             Location/Qualifiers
     source          1..12635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1378..1727
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    1386..1404
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1422..1440
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1458..1476
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1494..1512
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1530..1548
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1566..1584
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1602..1620
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1666..1690
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1842..1907
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3348..4058
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     polyA_signal    4080..4304
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4337..4472
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    4577..4598
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4613..4618,4619..4636,4637..4643)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4651..4667
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4675..4691
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5032..5051
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    5050..5105
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     5151..5170
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(5249..5995)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKGKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6092..6111)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          6119..7127
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(6173..6195)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(7128..7403)
                     /label=chicken beta-actin promoter
     enhancer        7406..7709
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    7773..7806
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        7870..8369
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7905..7928)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8302..8321
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        8381..8428
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8447..9046
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8447..8466)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8943..8963
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9084..9308
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    9363..9396
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(10414..10430)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10438..10454
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10462..10468,10469..10486,10487..10492))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10507..10528
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10645..10662)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10816..11404)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10896..10915)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11575..12366,12367..12435))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12198..12217
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12436..12540)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtattta aatactgccc ctaggagatg
      421 gatactagca tttgaaactg aggctcatat gtgttatcac tcaaagtgag gtcagactgc
      481 taggaagtta gggaagtggg atttgagcca ctgaaggtcg gaatctgaaa tctggcgagg
      541 tggtaggaag aggtaggaag gtacccaaca gcttcgaaca ggaggaactc ccagatcctt
      601 acaccaaaaa ccacttaggg ttttttgttt tgtttttgtt ttttaaattt cttttctttt
      661 cttccatggg caaagaaaat gactcaggaa gactcagtgg caaccctaga ctcagggcct
      721 cggaagaggc ccacctctgc atcatcctct ccggagcccc ctgagttcag cactttccgg
      781 gcctgccagt gaggctgacg aatgaggacc actttatcca gttccttccc tcccactgcc
      841 agaggctgca catctgtcca gagacttggc agtggaggta gggtgggggt gggaatcaag
      901 ccatagcttt cttagggaag cactggccaa aggaagggga ctcctagagt tgtaaccttc
      961 ctcacagaag acaagaaaat gagttggggt atcagcctca ggctagacag agagccagaa
     1021 cctcttcaca gattcccaga tcaccggaga agtcactatt gaatccggac acccagtcag
     1081 gcacaagagg tctacattct ctaactcctt tccagtgctt ccccaacggt cacttacttc
     1141 cagactgcgt gttttatttt taggagagat gtgtatattt tttgttgctg ttgttgtttc
     1201 tagatagggt ctcactgtgt agccctggct tttctggaac tcactgtgta gagcaagcca
     1261 gcctcaaact catagatcca cctgcctctg cctccagatc gctagaatta aagttactgc
     1321 cataacacct aacatttaaa tcctgcaggg tatcacgagg ccctttcgtc ttcactcgag
     1381 tttactccct atcagtgata gagaacgtat gaagagttta ctccctatca gtgatagaga
     1441 acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt tactccctat
     1501 cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac gtatctacag
     1561 tttactccct atcagtgata gagaacgtat atccagttta ctccctatca gtgatagaga
     1621 acgtatgtcg aggtaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa
     1681 ccgtcagatc gcctggagca attccacaac acttttgtct tatacttacg cgtctgcagg
     1741 atatcaagct tgcggtaccg cggccccgaa ttcgagctcg gtacccgggg atcctctagt
     1801 cagctgacgc gtgctagcgc ggccgcatcg attccaccat ggactataag gaccacgacg
     1861 gagactacaa ggatcatgat attgattaca aagacgatga cgataaggtc gacttccagt
     1921 gtgagctttg cagttacaca tgtccccggc gttcaaattt ggatcgtcac atgaaaagcc
     1981 acactgatga gagaccacac aaatgccatc tgtgtggcag agcattcaga acagtgaccc
     2041 tcctgaggaa tcatctgaac acacacacag gtactcgtcc tcacaagtgc ccagactgcg
     2101 atatggcctt tgtgaccagt ggagaattgg tgcggcatcg tcgttataaa cacactcatg
     2161 agaaaccatt taagtgttcc atgtgtgatt atgccagtgt agaagtcagc aaattaaaac
     2221 gacacattcg ctctcatact ggagagcgcc cgttccagtg cagtttgtgc agttatgcca
     2281 gcagggacac atacaagctg aaaaggcata tgagaaccca ttcaggggaa aaaccttatg
     2341 aatgttatat ttgtcacgct cggtttaccc agagtggtac catgaagatg cacattttac
     2401 agaagcacac agaaaatgtg gccaaatttc attgtcccca ttgtgacact gtcatagccc
     2461 gaaaaagtga tttgggtgtc cacttgcgaa agcagcattc ctatattgaa cagggcaaaa
     2521 aatgtcgcta ctgtgatgct gtgtttcatg agcgatatgc tctcatccag catcaaaaat
     2581 cacacaaaaa tgagaagcgc ttcaagtgtg accagtgtga ttatgcttgt agacaggagc
     2641 ggcacatgat catgcacaag cgcactcaca cgggggagaa gccttatgcc tgcagccact
     2701 gcgacaagac cttccgccag aaacagctcc tcgacatgca tttcaagcgc tatcatgatc
     2761 ccaactttgt ccctgctgcc tttgtctgtt ccaagtgtgg gaaaacattc acccgccgga
     2821 acacaatggc aagacatgca gataactgtg ctggtccaga tggcgtagag ggggaaaatg
     2881 gaggggagac aaagaagagc aaacgaggaa gaaaaagaaa gatgcgatct aaaaaagaag
     2941 actcctctga cagtgaagaa aatgctgagc cggatctcga tgacaatgag gaggaggagg
     3001 agcctgctgt agaaattgaa cctgagccag agcctcagcc ccagcctccg cctccacctc
     3061 agcctgtggc cccggccccc ccacctgcca agaagagaag ggggcgacct cctggaagaa
     3121 ccaaccagcc caaacagaac cagccaacag ccatcattca ggtcgaagat cagaatacag
     3181 gtgcaattga gaacattata gttgaagtca aaaaggagcc agatgccgag cctgcggagg
     3241 gggaagaaga ggaggctcag gcagccacca cagacgcccc caacggagac ctcacgcctg
     3301 agatgatcct cagcatgatg gaccggctta aggggggagc aggaggcatg gtgtctaagg
     3361 gcgaagagct gatcaaggaa aatatgcgta tgaaggtggt catggaaggt tcggtcaacg
     3421 gccaccaatt caaatgcaca ggtgaaggag aaggcaatcc gtacatggga actcaaacca
     3481 tgaggatcaa agtcatcgag ggaggacccc tgccatttgc ctttgacatt cttgccacgt
     3541 cgttcatgta tggcagccgt acttttatca agtacccgaa aggcattcct gatttcttta
     3601 aacagtcctt tcctgagggt tttacttggg aaagagttac gagatacgaa gatggtggag
     3661 tcgtcaccgt catgcaggac accagccttg aggatggctg tctcgtttac cacgtccaag
     3721 tcagaggggt aaactttccc tccaatggtc ccgtgatgca gaagaagacc aagggttggg
     3781 agcctaatac agagatgatg tatccagcag atggtggtct gaggggatac actcatatgg
     3841 cactgaaagt tgatggtggt ggccatctgt cttgctcttt cgtaacaact tacaggtcaa
     3901 aaaagaccgt cgggaacatc aagatgcccg gtatccatgc cgttgatcac cgcctggaaa
     3961 ggttagagga aagtgacaat gaaatgttcg tagtacaacg cgaacacgca gttgccaagt
     4021 tcgccgggct tggtggtggg atggacgagc tgtacaagta agtttaaaca tagcctcgac
     4081 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     4141 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     4201 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     4261 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     4321 aaccttaatt aacctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
     4381 gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
     4441 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt
     4501 gctgactaat tgagatgcgg atccgctggc acgacaggtt tcccgactgg aaagcgggca
     4561 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     4621 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     4681 acagctatga ccatgattac gccaagcttg ggctgcaggt cgagggatct tcataagaga
     4741 agagggacag ctatgactgg gagtagtcag gagaggagga aaaatctggc tagtaaaaca
     4801 tgtaaggaaa attttaggga tgttaaagaa aaaaataaca caaaacaaaa tataaaaaaa
     4861 atctaacctc aagtcaaggc ttttctatgg aataaggaat ggacagcagg gggctgtttc
     4921 atatactgat gacctcttta tagccacctt tgttcatggc agccagcata tggcatatgt
     4981 tgccaaactc taaaccaaat actcattctg atgttttaaa tgatttgccc tcccatatgt
     5041 ccttccgagt gagagacaca aaaaattcca acacactatt gcaatgaaaa taaatttcct
     5101 ttattagcca gaagtcagat gctcaagggg cttcatgatg tccccataat ttttggcaga
     5161 gggaaaaaga tctcagtggt atttgtgagc cagggcattg gccacaccag ccaccacctt
     5221 ctgataggca gcctgcacct gagcgccgtt acccggggag catgtcaagg tcaaaatcgt
     5281 caagagcgtc agcaggcagc atatcaaggt caaagtcgtc aagggcatcg gctgggagca
     5341 tgtctaagtc aaaatcgtca agggcgtcgg tcggcccgcc gctttcgcac tttagctgtt
     5401 tctccaggcc acatatgatt agttccaggc cgaaaaggaa ggcaggttcg gctccctgcc
     5461 ggtcgaacag ctcaattgct tgtttcagaa gtgggggcat agaatcggtg gtaggtgtct
     5521 ctctttcctc ttttgctact tgatgctcct gttcctccaa tacgcagccc agtgtaaagt
     5581 ggcccacggc ggacagagcg tacagtgcgt tctccaggga gaagccttgc tgacacagga
     5641 acgcgagctg attttccagg gtttcgtact gtttctctgt tgggcgggtg ccgagatgca
     5701 ctttagcccc gtcgcgatgt gagaggagag cacagcggta tgacttggcg ttgttccgca
     5761 gaaagtcttg ccatgactcg ccttccaggg ggcaggagtg ggtatgatgc ctgtccagca
     5821 tctcgattgg cagggcatcg agcagggccc gcttgttctt cacgtgccag tacagggtag
     5881 gctgctcaac tcccagcttt tgagcgagtt tccttgtcgt caggccttcg ataccgactc
     5941 cattgagtaa ttccagagca gagtttatga ctttgccctt gtccagtcta gacatggtgg
     6001 cggcggatcc cgggcccgcg gtaccgcaag cttgatatcc tgcagacgcg tgagaattct
     6061 cgaggaattc tttgccaaaa tgatgagaca gcacaataac cagcacgttg cccaggagct
     6121 gtaggaaaaa gaagaaggca tgaacatggt tagcagaggc tctagagccg ccggtcacac
     6181 gccagaagcc gaaccccgcc ctgccccgtc ccccccgaag gcagccgtcc ccctgcggca
     6241 gccccgaggc tggagatgga gaaggggacg gcggcgcggc gacgcacgaa ggccctcccc
     6301 gcccatttcc ttcctgccgg cgccgcaccg cttcgcccgc gcccgctaga gggggtgcgg
     6361 cggcgcctcc cagatttcgg ctccgccaga tttgggacaa aggaagtccc tgcgccctct
     6421 cgcacgatta ccataaaagg caatggctgc ggctcgccgc gcctcgacag ccgccggcgc
     6481 tccggggccg ccgcgcccct cccccgagcc ctccccggcc cgaggcggcc ccgccccgcc
     6541 cggcaccccc acctgccgcc accccccgcc cggcacggcg agccccgcgc cacgccccgc
     6601 acggagcccc gcacccgaag ccgggccgtg ctcagcaact cggggagggg ggtgcagggg
     6661 ggggttacag cccgaccgcc gcgcccacac cccctgctca cccccccacg cacacacccc
     6721 gcacgcagcc tttgttcccc tcgcagcccc cccgcaccgc ggggcaccgc ccccggccgc
     6781 gctcccctcg cgcacacgcg gagcgcacaa agccccgcgc cgcgcccgca gcgctcacag
     6841 ccgccgggca gcgcgggccg cacgcggcgc tccccacgca cacacacacg cacgcacccc
     6901 ccgagccgct cccccccgca caaagggccc tcccggagcc ctttaaggct ttcacgcagc
     6961 cacagaaaag aaacgagccg tcattaaacc aagcgctaat tacagcccgg aggagaaggg
     7021 ccgtcccgcc cgctcacctg tgggagtaac gcggtcagtc agagccgggg cgggcggcgc
     7081 gaggcggcgc ggagcggggc acggggcgaa ggcaacgcag cgactcccgc ccgccgcgcg
     7141 cttcgctttt tatagggccg ccgccgccgc cgcctcgcca taaaaggaaa ctttcggagc
     7201 gcgccgctct gattggctgc cgccgcacct ctccgcctcg ccccgccccg cccctcgccc
     7261 cgccccgccc cgcctggcgc gcgccccccc cccccccgcc cccatcgctg cacaaaataa
     7321 ttaaaaaata aataaataca aaattggggg tggggagggg ggggagatgg ggagagtgaa
     7381 gcagaacgtg gggctcacct cgacccatgg taatagcgat gactaatacg tagatgtact
     7441 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
     7501 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
     7561 gggcagttta ccgtaaatag tccacccatt gacgtcaatg gaaagtccct attggcgtta
     7621 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
     7681 gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg
     7741 accccgtaat tgattactat taataactag tcgaagttcc tattctctag aaagtatagg
     7801 aacttcaggt ctgaagagga gtttacgtcc agccaagcta gcttggctgc aggtcgtcga
     7861 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     7921 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
     7981 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     8041 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     8101 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     8161 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
     8221 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
     8281 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     8341 tctcctcttc ctcatctccg ggcctttcga cctgcagcct gttgacaatt aatcatcggc
     8401 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatga ccgagtacaa
     8461 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     8521 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     8581 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     8641 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     8701 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     8761 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     8821 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     8881 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     8941 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     9001 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagggg atcaattctc
     9061 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     9121 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     9181 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     9241 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     9301 ctctatggct tctgaggcgg aaagaaccag ctggggctcg actagagctt gcggaaccct
     9361 tcgaagttcc tattctctag aaagtatagg aacttcggcg cgccatttaa attttgggat
     9421 gttttctgtt tgttttgaga tagggttttt cagtgtagtc ctggctgtcc tagaactcac
     9481 tctgtagacc aggctggcct ggaactcaag gactgccagc ctctgcctcc tgagcatcgg
     9541 gattaaaggt gtgtgccacc actgcccagc agagtgcgtt tttactcttc actttgtggg
     9601 tcctggctgc ctgagattgt gtgcttgcac cattagatgg gagggatgac ttgggaaagg
     9661 gttgagtctt ttgtgagtct tgcggaggcc caggaggggg tacgtaaatt agagttctaa
     9721 gaggcccagc aaaagtgaac aagtgactgg ccactgtgag ttcactgggg gacctgagag
     9781 ttaactagtg aaaaggagag gtcctagtgg agaagctaaa gaattaaagt tactgccttg
     9841 aagttctttg tgaaggccaa gctctggagc gcggcttgaa aagcctttat cccagagcag
     9901 catgggaagg ggccccagct gggtgcgagc ctgcaaacca gagcacacct atcacagggc
     9961 cttttcttac cagtgggacg ggatcaaggc ggagcttacc tgggaacgta cagggcggaa
    10021 ccttaacttg ggggcgtggc cgaatcacct gggtttggaa acggttcttg tctttcctca
    10081 agctcaggag aggttgtttg caggacactg gcccggaggg gcccgaactc tccgagggag
    10141 ggtttcgtgg atcagaacac cgtcccttga caaaaagatg cagagcaccg tctgcattct
    10201 gctagtaacc gtccctcctg ggggcggagc cagccctcct ccgggtgtat aaagtagctg
    10261 agcgcctcct tctgacaaac gcctcacccc caagagtact gggggagtac gcagcacgcc
    10321 agcctagggg cggagctcag ccctggagga ggcgtttctt cttagttctc cgactcacgc
    10381 ctctccactt ttcatttaaa tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
    10441 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
    10501 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
    10561 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
    10621 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
    10681 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
    10741 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
    10801 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    10861 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    10921 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    10981 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    11041 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    11101 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    11161 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    11221 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
    11281 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    11341 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    11401 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    11461 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    11521 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    11581 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    11641 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    11701 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    11761 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    11821 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    11881 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    11941 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    12001 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    12061 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    12121 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    12181 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    12241 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    12301 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    12361 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    12421 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    12481 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    12541 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    12601 ctataaaaat aggcgtatca cgaggccctt tcgtc
//
