LOCUS       sequence-301982-       13427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector to introduce a constitutive rtta3G cassette and a 
            DOX-inducible mutant CTCF mouse cDNA (all Zinc fingers point 
            mutated), targeting the mouse TIGRE acceptor locus. Use with Addgene
            #92144 sgRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13427)
  AUTHORS   Nora EP, Caccianini L, Fudenberg G, So K, Kameswaran V, Nagle A, 
            Uebersohn A, Hajj B, Saux AL, Coulon A, Mirny LA, Pollard KS, Dahan 
            M, Bruneau BG
  TITLE     Molecular basis of CTCF binding polarity in genome folding.
  JOURNAL   Nat Commun. 2020 Nov 5;11(1):5612. doi: 10.1038/s41467-020-19283-x.
  PUBMED    33154377
REFERENCE   2  (bases 1 to 13427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-11-5"; volume: "11(1):5612. doi"; pages: " 
            10.1038/s41467-020-19283-x"
COMMENT     Sequence Label: pEN515 -
            pTRE3G-CTCF(ZFmut)-mRuby2-CAGGS-rtta3...
FEATURES             Location/Qualifiers
     source          1..13427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1378..1727
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    1386..1404
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1422..1440
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1458..1476
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1494..1512
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1530..1548
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1566..1584
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1602..1620
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1666..1690
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1842..1907
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4140..4850
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     polyA_signal    4872..5096
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5129..5264
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    5369..5390
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5405..5410,5411..5428,5429..5435)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5443..5459
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5467..5483
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5824..5843
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    5842..5897
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     5943..5962
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(6041..6787)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKGKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6884..6903)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          6911..7919
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(6965..6987)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(7920..8195)
                     /label=chicken beta-actin promoter
     enhancer        8198..8501
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    8565..8598
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        8662..9161
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8697..8720)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9094..9113
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        9173..9220
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9239..9838
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9239..9258)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9735..9755
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9876..10100
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    10155..10188
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(11206..11222)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11230..11246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11254..11260,11261..11278,11279..11284))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11299..11320
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11437..11454)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11608..12196)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11688..11707)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12367..13158,13159..13227))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12990..13009
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13228..13332)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtattta aatactgccc ctaggagatg
      421 gatactagca tttgaaactg aggctcatat gtgttatcac tcaaagtgag gtcagactgc
      481 taggaagtta gggaagtggg atttgagcca ctgaaggtcg gaatctgaaa tctggcgagg
      541 tggtaggaag aggtaggaag gtacccaaca gcttcgaaca ggaggaactc ccagatcctt
      601 acaccaaaaa ccacttaggg ttttttgttt tgtttttgtt ttttaaattt cttttctttt
      661 cttccatggg caaagaaaat gactcaggaa gactcagtgg caaccctaga ctcagggcct
      721 cggaagaggc ccacctctgc atcatcctct ccggagcccc ctgagttcag cactttccgg
      781 gcctgccagt gaggctgacg aatgaggacc actttatcca gttccttccc tcccactgcc
      841 agaggctgca catctgtcca gagacttggc agtggaggta gggtgggggt gggaatcaag
      901 ccatagcttt cttagggaag cactggccaa aggaagggga ctcctagagt tgtaaccttc
      961 ctcacagaag acaagaaaat gagttggggt atcagcctca ggctagacag agagccagaa
     1021 cctcttcaca gattcccaga tcaccggaga agtcactatt gaatccggac acccagtcag
     1081 gcacaagagg tctacattct ctaactcctt tccagtgctt ccccaacggt cacttacttc
     1141 cagactgcgt gttttatttt taggagagat gtgtatattt tttgttgctg ttgttgtttc
     1201 tagatagggt ctcactgtgt agccctggct tttctggaac tcactgtgta gagcaagcca
     1261 gcctcaaact catagatcca cctgcctctg cctccagatc gctagaatta aagttactgc
     1321 cataacacct aacatttaaa tcctgcaggg tatcacgagg ccctttcgtc ttcactcgag
     1381 tttactccct atcagtgata gagaacgtat gaagagttta ctccctatca gtgatagaga
     1441 acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt tactccctat
     1501 cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac gtatctacag
     1561 tttactccct atcagtgata gagaacgtat atccagttta ctccctatca gtgatagaga
     1621 acgtatgtcg aggtaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa
     1681 ccgtcagatc gcctggagca attccacaac acttttgtct tatacttacg cgtctgcagg
     1741 atatcaagct tgcggtaccg cggccccgaa ttcgagctcg gtacccgggg atcctctagt
     1801 cagctgacgc gtgctagcgc ggccgcatcg attccaccat ggactataag gaccacgacg
     1861 gagactacaa ggatcatgat attgattaca aagacgatga cgataaggtc gacgaaggtg
     1921 aggcggttga agccattgtg gaggagtctg aaactttcat taaaggaaaa gaaagaaaga
     1981 cttaccagag acgccgggaa gggggccagg aagaagatgc ttgccacctg ccccagaacc
     2041 agacagatgg gggtgaggtg gtccaggatg tcaacagcag tgtacagatg gtaatgatgg
     2101 aacagctgga tcctaccctt ctccagatga agactgaagt catggagggt acagtggctc
     2161 cggaagcaga ggctgcagtg gacgataccc agatcataac cttgcaggtt gtaaatatgg
     2221 aggaacagcc cattaacata ggagagcttc agcttgtcca agtacctgtt cctgtgacgg
     2281 tacctgttgc tactacttca gtagaagaac ttcagggggc ttatgagaat gaagtgtcta
     2341 aagagggcct tgcagaaagt gaaccgatga tatgtcacac cttacctttg cctgaaggat
     2401 ttcaggtggt gaaagtgggg gccaatggag aagtggagac actagagcag ggcgagcttc
     2461 ctcctcagga agactctagc tggcaaaaag acccagacta tcagccacca gccaaaaaaa
     2521 caaagaaaac caaaaagagc aaacttcgtt acacagaaga gggcaaagac gtggatgtgt
     2581 ctgtgtatga ttttgaggaa gaacagcagg aaggactgct gtctgaggtt aatgcagaga
     2641 aagtagttgg taatatgaag cctccgaagc caacaaaaat taaaaaaaaa ggtgtaaaga
     2701 aaacattcca gtgtgagctt tgcagttaca catgtccccg gcgttcaaat ttggatcgtc
     2761 acatgaaaag ccgaactgat gagagaccac acaaatgcca tctgtgtggc agagcattca
     2821 gaacagtgac cctcctgagg aatcatctga acacacgaac aggtactcgt cctcacaagt
     2881 gcccagactg cgatatggcc tttgtgacca gtggagaatt ggtgcggcat cgtcgttata
     2941 aacacactcg agagaaacca tttaagtgtt ccatgtgtga ttatgccagt gtagaagtca
     3001 gcaaattaaa acgacacatt cgctctcgaa ctggagagcg cccgttccag tgcagtttgt
     3061 gcagttatgc cagcagggac acatacaagc tgaaaaggca tatgagaacc cgatcagggg
     3121 aaaaacctta tgaatgttat atttgtcacg ctcggtttac ccagagtggt accatgaaga
     3181 tgcacatttt acagaagcga acagaaaatg tggccaaatt tcattgtccc cattgtgaca
     3241 ctgtcatagc ccgaaaaagt gatttgggtg tccacttgcg aaagcagcga tcctatattg
     3301 aacagggcaa aaaatgtcgc tactgtgatg ctgtgtttca tgagcgatat gctctcatcc
     3361 agcatcaaaa atcacgaaaa aatgagaagc gcttcaagtg tgaccagtgt gattatgctt
     3421 gtagacagga gcggcacatg atcatgcaca agcgcactcg aacgggggag aagccttatg
     3481 cctgcagcca ctgcgacaag accttccgcc agaaacagct cctcgacatg catttcaagc
     3541 gctatcgaga tcccaacttt gtccctgctg cctttgtctg ttccaagtgt gggaaaacat
     3601 tcacccgccg gaacacaatg gcaagacgag cagataactg tgctggtcca gatggcgtag
     3661 agggggaaaa tggaggggag acaaagaaga gcaaacgagg aagaaaaaga aagatgcgat
     3721 ctaaaaaaga agactcctct gacagtgaag aaaatgctga gccggatctc gatgacaatg
     3781 aggaggagga ggagcctgct gtagaaattg aacctgagcc agagcctcag ccccagcctc
     3841 cgcctccacc tcagcctgtg gccccggccc ccccacctgc caagaagaga agggggcgac
     3901 ctcctggaag aaccaaccag cccaaacaga accagccaac agccatcatt caggtcgaag
     3961 atcagaatac aggtgcaatt gagaacatta tagttgaagt caaaaaggag ccagatgccg
     4021 agcctgcgga gggggaagaa gaggaggctc aggcagccac cacagacgcc cccaacggag
     4081 acctcacgcc tgagatgatc ctcagcatga tggaccggct taagggggga gcaggaggca
     4141 tggtgtctaa gggcgaagag ctgatcaagg aaaatatgcg tatgaaggtg gtcatggaag
     4201 gttcggtcaa cggccaccaa ttcaaatgca caggtgaagg agaaggcaat ccgtacatgg
     4261 gaactcaaac catgaggatc aaagtcatcg agggaggacc cctgccattt gcctttgaca
     4321 ttcttgccac gtcgttcatg tatggcagcc gtacttttat caagtacccg aaaggcattc
     4381 ctgatttctt taaacagtcc tttcctgagg gttttacttg ggaaagagtt acgagatacg
     4441 aagatggtgg agtcgtcacc gtcatgcagg acaccagcct tgaggatggc tgtctcgttt
     4501 accacgtcca agtcagaggg gtaaactttc cctccaatgg tcccgtgatg cagaagaaga
     4561 ccaagggttg ggagcctaat acagagatga tgtatccagc agatggtggt ctgaggggat
     4621 acactcatat ggcactgaaa gttgatggtg gtggccatct gtcttgctct ttcgtaacaa
     4681 cttacaggtc aaaaaagacc gtcgggaaca tcaagatgcc cggtatccat gccgttgatc
     4741 accgcctgga aaggttagag gaaagtgaca atgaaatgtt cgtagtacaa cgcgaacacg
     4801 cagttgccaa gttcgccggg cttggtggtg ggatggacga gctgtacaag taagtttaaa
     4861 catagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     4921 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     4981 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     5041 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     5101 tgaggcggaa agaaccttaa ttaacctagg cctccaaaaa agcctcctca ctacttctgg
     5161 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
     5221 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
     5281 gggactatgg ttgctgacta attgagatgc ggatccgctg gcacgacagg tttcccgact
     5341 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     5401 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     5461 ttcacacagg aaacagctat gaccatgatt acgccaagct tgggctgcag gtcgagggat
     5521 cttcataaga gaagagggac agctatgact gggagtagtc aggagaggag gaaaaatctg
     5581 gctagtaaaa catgtaagga aaattttagg gatgttaaag aaaaaaataa cacaaaacaa
     5641 aatataaaaa aaatctaacc tcaagtcaag gcttttctat ggaataagga atggacagca
     5701 gggggctgtt tcatatactg atgacctctt tatagccacc tttgttcatg gcagccagca
     5761 tatggcatat gttgccaaac tctaaaccaa atactcattc tgatgtttta aatgatttgc
     5821 cctcccatat gtccttccga gtgagagaca caaaaaattc caacacacta ttgcaatgaa
     5881 aataaatttc ctttattagc cagaagtcag atgctcaagg ggcttcatga tgtccccata
     5941 atttttggca gagggaaaaa gatctcagtg gtatttgtga gccagggcat tggccacacc
     6001 agccaccacc ttctgatagg cagcctgcac ctgagcgccg ttacccgggg agcatgtcaa
     6061 ggtcaaaatc gtcaagagcg tcagcaggca gcatatcaag gtcaaagtcg tcaagggcat
     6121 cggctgggag catgtctaag tcaaaatcgt caagggcgtc ggtcggcccg ccgctttcgc
     6181 actttagctg tttctccagg ccacatatga ttagttccag gccgaaaagg aaggcaggtt
     6241 cggctccctg ccggtcgaac agctcaattg cttgtttcag aagtgggggc atagaatcgg
     6301 tggtaggtgt ctctctttcc tcttttgcta cttgatgctc ctgttcctcc aatacgcagc
     6361 ccagtgtaaa gtggcccacg gcggacagag cgtacagtgc gttctccagg gagaagcctt
     6421 gctgacacag gaacgcgagc tgattttcca gggtttcgta ctgtttctct gttgggcggg
     6481 tgccgagatg cactttagcc ccgtcgcgat gtgagaggag agcacagcgg tatgacttgg
     6541 cgttgttccg cagaaagtct tgccatgact cgccttccag ggggcaggag tgggtatgat
     6601 gcctgtccag catctcgatt ggcagggcat cgagcagggc ccgcttgttc ttcacgtgcc
     6661 agtacagggt aggctgctca actcccagct tttgagcgag tttccttgtc gtcaggcctt
     6721 cgataccgac tccattgagt aattccagag cagagtttat gactttgccc ttgtccagtc
     6781 tagacatggt ggcggcggat cccgggcccg cggtaccgca agcttgatat cctgcagacg
     6841 cgtgagaatt ctcgaggaat tctttgccaa aatgatgaga cagcacaata accagcacgt
     6901 tgcccaggag ctgtaggaaa aagaagaagg catgaacatg gttagcagag gctctagagc
     6961 cgccggtcac acgccagaag ccgaaccccg ccctgccccg tcccccccga aggcagccgt
     7021 ccccctgcgg cagccccgag gctggagatg gagaagggga cggcggcgcg gcgacgcacg
     7081 aaggccctcc ccgcccattt ccttcctgcc ggcgccgcac cgcttcgccc gcgcccgcta
     7141 gagggggtgc ggcggcgcct cccagatttc ggctccgcca gatttgggac aaaggaagtc
     7201 cctgcgccct ctcgcacgat taccataaaa ggcaatggct gcggctcgcc gcgcctcgac
     7261 agccgccggc gctccggggc cgccgcgccc ctcccccgag ccctccccgg cccgaggcgg
     7321 ccccgccccg cccggcaccc ccacctgccg ccaccccccg cccggcacgg cgagccccgc
     7381 gccacgcccc gcacggagcc ccgcacccga agccgggccg tgctcagcaa ctcggggagg
     7441 ggggtgcagg ggggggttac agcccgaccg ccgcgcccac accccctgct caccccccca
     7501 cgcacacacc ccgcacgcag cctttgttcc cctcgcagcc cccccgcacc gcggggcacc
     7561 gcccccggcc gcgctcccct cgcgcacacg cggagcgcac aaagccccgc gccgcgcccg
     7621 cagcgctcac agccgccggg cagcgcgggc cgcacgcggc gctccccacg cacacacaca
     7681 cgcacgcacc ccccgagccg ctcccccccg cacaaagggc cctcccggag ccctttaagg
     7741 ctttcacgca gccacagaaa agaaacgagc cgtcattaaa ccaagcgcta attacagccc
     7801 ggaggagaag ggccgtcccg cccgctcacc tgtgggagta acgcggtcag tcagagccgg
     7861 ggcgggcggc gcgaggcggc gcggagcggg gcacggggcg aaggcaacgc agcgactccc
     7921 gcccgccgcg cgcttcgctt tttatagggc cgccgccgcc gccgcctcgc cataaaagga
     7981 aactttcgga gcgcgccgct ctgattggct gccgccgcac ctctccgcct cgccccgccc
     8041 cgcccctcgc cccgccccgc cccgcctggc gcgcgccccc cccccccccg cccccatcgc
     8101 tgcacaaaat aattaaaaaa taaataaata caaaattggg ggtggggagg ggggggagat
     8161 ggggagagtg aagcagaacg tggggctcac ctcgacccat ggtaatagcg atgactaata
     8221 cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg
     8281 cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat
     8341 gtactgccaa gtgggcagtt taccgtaaat agtccaccca ttgacgtcaa tggaaagtcc
     8401 ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg
     8461 gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc
     8521 tatgaactaa tgaccccgta attgattact attaataact agtcgaagtt cctattctct
     8581 agaaagtata ggaacttcag gtctgaagag gagtttacgt ccagccaagc tagcttggct
     8641 gcaggtcgtc gaaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     8701 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     8761 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     8821 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     8881 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     8941 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     9001 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     9061 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     9121 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ctgttgacaa
     9181 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     9241 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     9301 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     9361 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     9421 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     9481 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     9541 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     9601 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     9661 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     9721 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     9781 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagg
     9841 ggatcaattc tctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     9901 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     9961 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
    10021 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
    10081 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct cgactagagc
    10141 ttgcggaacc cttcgaagtt cctattctct agaaagtata ggaacttcgg cgcgccattt
    10201 aaattttggg atgttttctg tttgttttga gatagggttt ttcagtgtag tcctggctgt
    10261 cctagaactc actctgtaga ccaggctggc ctggaactca aggactgcca gcctctgcct
    10321 cctgagcatc gggattaaag gtgtgtgcca ccactgccca gcagagtgcg tttttactct
    10381 tcactttgtg ggtcctggct gcctgagatt gtgtgcttgc accattagat gggagggatg
    10441 acttgggaaa gggttgagtc ttttgtgagt cttgcggagg cccaggaggg ggtacgtaaa
    10501 ttagagttct aagaggccca gcaaaagtga acaagtgact ggccactgtg agttcactgg
    10561 gggacctgag agttaactag tgaaaaggag aggtcctagt ggagaagcta aagaattaaa
    10621 gttactgcct tgaagttctt tgtgaaggcc aagctctgga gcgcggcttg aaaagccttt
    10681 atcccagagc agcatgggaa ggggccccag ctgggtgcga gcctgcaaac cagagcacac
    10741 ctatcacagg gccttttctt accagtggga cgggatcaag gcggagctta cctgggaacg
    10801 tacagggcgg aaccttaact tgggggcgtg gccgaatcac ctgggtttgg aaacggttct
    10861 tgtctttcct caagctcagg agaggttgtt tgcaggacac tggcccggag gggcccgaac
    10921 tctccgaggg agggtttcgt ggatcagaac accgtccctt gacaaaaaga tgcagagcac
    10981 cgtctgcatt ctgctagtaa ccgtccctcc tgggggcgga gccagccctc ctccgggtgt
    11041 ataaagtagc tgagcgcctc cttctgacaa acgcctcacc cccaagagta ctgggggagt
    11101 acgcagcacg ccagcctagg ggcggagctc agccctggag gaggcgtttc ttcttagttc
    11161 tccgactcac gcctctccac ttttcattta aatggcgtaa tcatggtcat agctgtttcc
    11221 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
    11281 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
    11341 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
    11401 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    11461 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    11521 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    11581 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    11641 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    11701 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    11761 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    11821 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    11881 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    11941 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    12001 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
    12061 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    12121 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    12181 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    12241 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    12301 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    12361 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    12421 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    12481 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    12541 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    12601 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    12661 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    12721 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    12781 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    12841 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    12901 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    12961 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    13021 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    13081 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    13141 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    13201 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    13261 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    13321 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
    13381 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
//
