LOCUS       sequence-302010-        7113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mutisite Gateway mediated vector construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7113)
  AUTHORS   Yoshimatsu S, Sone T, Nakajima M, Sato T, Okochi R, Ishikawa M, 
            Nakamura M, Sasaki E, Shiozawa S, Okano H
  TITLE     A versatile toolbox for knock-in gene targeting based on the 
            Multisite Gateway technology.
  JOURNAL   PLoS One. 2019 Aug 27;14(8):e0221164. doi: 
            10.1371/journal.pone.0221164. eCollection 2019.
  PUBMED    31454364
REFERENCE   2  (bases 1 to 7113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-08-27"; volume: "14(8):e0221164. doi"; pages: " 
            10.1371/journal.pone.0221164. eCollection 2019"
COMMENT     Sequence Label: pENTR-L3-Csy4-Cas9-L2
FEATURES             Location/Qualifiers
     source          1..7113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(201..220)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             212..1021
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(279..298)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1164..1752
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1653..1672
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1887..1914)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2006..2092)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    2146..2241
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      2270..2279
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2276..2836
                     /codon_start=1
                     /product="CRISPR-associated endoribonuclease responsible 
                     for pre-crRNA processing in Pseudomonas aeruginosa 
                     (Haurwitz et al., 2010)"
                     /label=Csy4
                     /translation="MDHYLDIRLRPDPEFPPAQLMSVLFGKLHQALVAQGGDRIGVSFP
                     DLDESRSRLGERLRIHASADDLRALLARPWLEGLRDHLQFGEPAVVPHPTPYRQVSRVQ
                     AKSNPERLRRRLMRRHDLSEEEARKRIPDTVARALDLPFVTLRSQSTGQHFRLFIRHGP
                     LQVTAEEGGFTCYGLSKGGFVPWF"
     CDS             2837..2890
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2891..6292,6293..6295,6296..6892,6893,6894..6896,
                     6897..6898,6899..6901,6902..6994)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2891..6994
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2891..6994
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7001..7021
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7028..7093
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(join(7103..7113,1..89))
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
       61 actatcagtc aaaataaaat cattatttgc catccagctg cagctctggc ccgtgtctca
      121 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
      181 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
      241 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
      301 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
      361 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
      421 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
      481 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
      541 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
      601 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
      661 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
      721 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
      781 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
      841 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
      901 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
      961 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     1021 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     1081 cgcaagctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1141 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1201 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1261 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1321 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1381 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1441 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1501 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1561 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1621 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1681 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     1741 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     1801 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgct
     1861 agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag
     1921 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca
     1981 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac
     2041 aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg
     2101 cagttcccta ctctcgcgtt aacgctagca tggatctcgg gccccaaata atgattttat
     2161 tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt tttataatgc
     2221 caactttgta taataaagtt gggtaccgcg ggcccgggat ccaccggtcg ccaccatgga
     2281 ccactacctc gacattcgct tgcgaccgga cccggaattt cccccggcgc aactcatgag
     2341 cgtgctcttc ggcaagctcc accaggccct ggtggcacag ggcggggaca ggatcggcgt
     2401 gagcttcccc gacctcgacg aaagccgctc ccggctgggc gagcgcctgc gcattcatgc
     2461 ctcggcggac gaccttcgtg ccctgctcgc ccggccctgg ctggaagggt tgcgggacca
     2521 tctgcaattc ggagaaccgg cagtcgtgcc tcaccccaca ccgtaccgtc aggtcagtcg
     2581 ggttcaggcg aaaagcaatc cggaacgcct gcggcggcgg ctcatgcgcc ggcacgatct
     2641 gagtgaggag gaggctcgga aacgcattcc cgatacggtc gcgagagcct tggacctgcc
     2701 cttcgtcacg ctacgcagcc agagcaccgg acagcacttc cgtctcttca tccgccacgg
     2761 gccgttgcag gtgacggcag aggaaggagg attcacctgt tacgggttga gcaaaggagg
     2821 tttcgttccc tggttcgagg gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa
     2881 tcctggccca atggataaaa agtattctat tggtttagac atcggcacta attccgttgg
     2941 atgggctgtc ataaccgatg aatacaaagt accttcaaag aaatttaagg tgttggggaa
     3001 cacagaccgt cattcgatta aaaagaatct tatcggtgcc ctcctattcg atagtggcga
     3061 aacggcagag gcgactcgcc tgaaacgaac cgctcggaga aggtatacac gtcgcaagaa
     3121 ccgaatatgt tacttacaag aaatttttag caatgagatg gccaaagttg acgattcttt
     3181 ctttcaccgt ttggaagagt ccttccttgt cgaagaggac aagaaacatg aacggcaccc
     3241 catctttgga aacatagtag atgaggtggc atatcatgaa aagtacccaa cgatttatca
     3301 cctcagaaaa aagctagttg actcaactga taaagcggac ctgaggttaa tctacttggc
     3361 tcttgcccat atgataaagt tccgtgggca ctttctcatt gagggtgatc taaatccgga
     3421 caactcggat gtcgacaaac tgttcatcca gttagtacaa acctataatc agttgtttga
     3481 agagaaccct ataaatgcaa gtggcgtgga tgcgaaggct attcttagcg cccgcctctc
     3541 taaatcccga cggctagaaa acctgatcgc acaattaccc ggagagaaga aaaatgggtt
     3601 gttcggtaac cttatagcgc tctcactagg cctgacacca aattttaagt cgaacttcga
     3661 cttagctgaa gatgccaaat tgcagcttag taaggacacg tacgatgacg atctcgacaa
     3721 tctactggca caaattggag atcagtatgc ggacttattt ttggctgcca aaaaccttag
     3781 cgatgcaatc ctcctatctg acatactgag agttaatact gagattacca aggcgccgtt
     3841 atccgcttca atgatcaaaa ggtacgatga acatcaccaa gacttgacac ttctcaaggc
     3901 cctagtccgt cagcaactgc ctgagaaata taaggaaata ttctttgatc agtcgaaaaa
     3961 cgggtacgca ggttatattg acggcggagc gagtcaagag gaattctaca agtttatcaa
     4021 acccatatta gagaagatgg atgggacgga agagttgctt gtaaaactca atcgcgaaga
     4081 tctactgcga aagcagcgga ctttcgacaa cggtagcatt ccacatcaaa tccacttagg
     4141 cgaattgcat gctatactta gaaggcagga ggatttttat ccgttcctca aagacaatcg
     4201 tgaaaagatt gagaaaatcc taacctttcg cataccttac tatgtgggac ccctggcccg
     4261 agggaactct cggttcgcat ggatgacaag aaagtccgaa gaaacgatta ctccatggaa
     4321 ttttgaggaa gttgtcgata aaggtgcgtc agctcaatcg ttcatcgaga ggatgaccaa
     4381 ctttgacaag aatttaccga acgaaaaagt attgcctaag cacagtttac tttacgagta
     4441 tttcacagtg tacaatgaac tcacgaaagt taagtatgtc actgagggca tgcgtaaacc
     4501 cgcctttcta agcggagaac agaagaaagc aatagtagat ctgttattca agaccaaccg
     4561 caaagtgaca gttaagcaat tgaaagagga ctactttaag aaaattgaat gcttcgattc
     4621 tgtcgagatc tccggggtag aagatcgatt taatgcgtca cttggtacgt atcatgacct
     4681 cctaaagata attaaagata aggacttcct ggataacgaa gagaatgaag atatcttaga
     4741 agatatagtg ttgactctta ccctctttga agatcgggaa atgattgagg aaagactaaa
     4801 aacatacgct cacctgttcg acgataaggt tatgaaacag ttaaagaggc gtcgctatac
     4861 gggctgggga cgattgtcgc ggaaacttat caacgggata agagacaagc aaagtggtaa
     4921 aactattctc gattttctaa agagcgacgg cttcgccaat aggaacttta tgcagctgat
     4981 ccatgatgac tctttaacct tcaaagagga tatacaaaag gcacaggttt ccggacaagg
     5041 ggactcattg cacgaacata ttgcgaatct tgctggttcg ccagccatca aaaagggcat
     5101 actccagaca gtcaaagtag tggatgagct agttaaggtc atgggacgtc acaaaccgga
     5161 aaacattgta atcgagatgg cacgcgaaaa tcaaacgact cagaaggggc aaaaaaacag
     5221 tcgagagcgg atgaagagaa tagaagaggg tattaaagaa ctgggcagcc agatcttaaa
     5281 ggagcatcct gtggaaaata cccaattgca gaacgagaaa ctttacctct attacctaca
     5341 aaatggaagg gacatgtatg ttgatcagga actggacata aaccgtttat ctgattacga
     5401 cgtcgatcac attgtacccc aatccttttt gaaggacgat tcaatcgaca ataaagtgct
     5461 tacacgctcg gataagaacc gagggaaaag tgacaatgtt ccaagcgagg aagtcgtaaa
     5521 gaaaatgaag aactattggc ggcagctcct aaatgcgaaa ctgataacgc aaagaaagtt
     5581 cgataactta actaaagctg agaggggtgg cttgtctgaa cttgacaagg ccggatttat
     5641 taaacgtcag ctcgtggaaa cccgccaaat cacaaagcat gttgcacaga tactagattc
     5701 ccgaatgaat acgaaatacg acgagaacga taagctgatt cgggaagtca aagtaatcac
     5761 tttaaagtca aaattggtgt cggacttcag aaaggatttt caattctata aagttaggga
     5821 gataaataac taccaccatg cgcacgacgc ttatcttaat gccgtcgtag ggaccgcact
     5881 cattaagaaa tacccgaagc tagaaagtga gtttgtgtat ggtgattaca aagtttatga
     5941 cgtccgtaag atgatcgcga aaagcgaaca ggagataggc aaggctacag ccaaatactt
     6001 cttttattct aacattatga atttctttaa gacggaaatc actctggcaa acggagagat
     6061 acgcaaacga cctttaattg aaaccaatgg ggagacaggt gaaatcgtat gggataaggg
     6121 ccgggacttc gcgacggtga gaaaagtttt gtccatgccc caagtcaaca tagtaaagaa
     6181 aactgaggtg cagaccggag ggttttcaaa ggaatcgatt cttccaaaaa ggaatagtga
     6241 taagctcatc gctcgtaaaa aggactggga cccgaaaaag tacggtggct tcgatagccc
     6301 tacagttgcc tattctgtcc tagtagtggc aaaagttgag aagggaaaat ccaagaaact
     6361 gaagtcagtc aaagaattat tggggataac gattatggag cgctcgtctt ttgaaaagaa
     6421 ccccatcgac ttccttgagg cgaaaggtta caaggaagta aaaaaggatc tcataattaa
     6481 actaccaaag tatagtctgt ttgagttaga aaatggccga aaacggatgt tggctagcgc
     6541 cggagagctt caaaagggga acgaactcgc actaccgtct aaatacgtga atttcctgta
     6601 tttagcgtcc cattacgaga agttgaaagg ttcacctgaa gataacgaac agaagcaact
     6661 ttttgttgag cagcacaaac attatctcga cgaaatcata gagcaaattt cggaattcag
     6721 taagagagtc atcctagctg atgccaatct ggacaaagta ttaagcgcat acaacaagca
     6781 cagggataaa cccatacgtg agcaggcgga aaatattatc catttgttta ctcttaccaa
     6841 cctcggcgct ccagccgcat tcaagtattt tgacacaacg atagatcgca aacgatacac
     6901 ttctaccaag gaggtgctag acgcgacact gattcaccaa tccatcacgg gattatatga
     6961 aactcggata gatttgtcac agcttggggg tgacggatcc cccaagaaga agaggaaagt
     7021 ctcgagcgac tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga
     7081 tgacgatgac aagtgaagcg gccgccagct ttc
//
