LOCUS       sequence-302025-        5539 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a rabbit Fc-ICAM1 fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Reimer D, Meyer-Hermann M, Rakhymzhan A, Steinmetz T, Tripal P, 
            Thomas J, Boettcher M, Mougiakakos D, Schulz SR, Urbanczyk S, Hauser
            AE, Niesner RA, Mielenz D
  TITLE     B Cell Speed and B-FDC Contacts in Germinal Centers Determine Plasma
            Cell Output via Swiprosin-1/EFhd2.
  JOURNAL   Cell Rep. 2020 Aug 11;32(6):108030. doi: 
            10.1016/j.celrep.2020.108030.
  PUBMED    32783949
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2020-08-11"; volume: "32(6):108030. doi"; pages: " 
            10.1016/j.celrep.2020.108030"
COMMENT     Sequence Label: pFUSE-rIgG-Fc2-ICAM1
FEATURES             Location/Qualifiers
     source          1..5539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..242
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             255..523
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     573..632
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     polyA_signal    2704..2825
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2715..2734)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2769..2788
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    2929..3323
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(3337..3711)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        4304..4607
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(4687..5275)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4767..4786)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5383..5431
                     /note="synthetic polyadenylation signal"
     misc_feature    5442..5533
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5482..5501
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
       61 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
      121 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
      181 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
      241 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
      301 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
      361 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
      421 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
      481 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
      541 ctacctgaga tcaccggcga aggagggcca ccatgtacag gatgcaactc ctgtcttgca
      601 ttgcactaag tcttgcactt gtcacgaatt cgcaggtatc catccatccc agagaagcct
      661 tcctgcccca gggtgggtcc gtgcaggtga actgttcttc ctcatgcaag gaggacctca
      721 gcctgggctt ggagactcag tggctgaaag atgagctcga gagtggaccc aactggaagc
      781 tgtttgagct gagcgagatc ggggaggaca gcagtccgct gtgctttgag aactgtggca
      841 ccgtgcagtc gtccgcttcc gctaccatca ccgtgtattc gtttccggag agtgtggagc
      901 tgagacctct gccagcctgg cagcaagtag gcaaggacct caccctgcgc tgccacgtgg
      961 atggtggagc accgcggacc cagctctcag cagtgctgct ccgtggggag gagatactga
     1021 gccgccagcc agtgggtggg caccccaagg accccaagga gatcacattc acggtgctgg
     1081 ctagcagagg ggaccacgga gccaatttct catgccgcac agaactggat ctcaggccgc
     1141 aagggctggc attgttctct aatgtctccg aggccaggag cctccggact ttcgatcttc
     1201 cagctaccat cccaaagctc gacacccctg acctcctgga ggtgggcacc cagcagaagt
     1261 tgttttgctc cctggaaggc ctgtttcctg cctctgaagc tcggatatac ctggagctgg
     1321 gaggccagat gccgacccag gagagcacaa acagcagtga ctctgtgtca gccactgcct
     1381 tggtagaggt gactgaggag ttcgacagaa ccctgccgct gcgctgcgtt ttggagctag
     1441 cggaccagat cctggagacg cagaggacct taacagtcta caacttttca gctccggtcc
     1501 tgaccctgag ccagctggag gtctcggaag ggagccaagt aactgtgaag tgtgaagccc
     1561 acagtgggtc gaaggtggtt cttctgagcg gcgtcgagcc taggccaccc accccgcagg
     1621 tccaattcac actgaatgcc agctcggagg atcacaaacg aagcttcttt tgctctgccg
     1681 ctctggaggt ggcgggaaag ttcctgttta aaaaccagac cctggaactg cacgtgctgt
     1741 atggtcctcg gctggacgag acggactgct tggggaactg gacctggcaa gaggggtctc
     1801 agcagactct gaaatgccag gcctggggga acccatctcc taagatgacc tgcagacgga
     1861 aggcagatgg tgccctgctg cccatcgggg tggtgaagtc tgtcaaacag gagatgaatg
     1921 gtacatacgt gtgccatgcc tttagctccc atgggaatgt caccaggaat gtgtacctga
     1981 cagtactgta ccactctcaa aataacagat ctagcaagcc cacgtgccca ccccctgaac
     2041 tcctgggggg accgtctgtc ttcatcttcc ccccaaaacc caaggacacc ctcatgatct
     2101 cacgcacccc cgaggtcaca tgcgtggtgg tggacgtgag ccaggatgac cccgaggtgc
     2161 agttcacatg gtacataaac aacgagcagg tgcgcaccgc ccggccgccg ctacgggagc
     2221 agcagttcaa cagcacgatc cgcgtggtca gcaccctccc catcgcgcac caggactggc
     2281 tgaggggcaa ggagttcaag tgcaaagtcc acaacaaggc actcccggcc cccatcgaga
     2341 aaaccatctc caaagccaga gggcagcccc tggagccgaa ggtctacacc atgggccctc
     2401 cccgggagga gctgagcagc aggtcggtca gcctgacctg catgatcaac ggcttctacc
     2461 cttccgacat ctcggtggag tgggagaaga acgggaaggc agaggacaac tacaagacca
     2521 cgccggccgt gctggacagc gacggctcct acttcctcta cagcaagctc tcagtgccca
     2581 cgagtgagtg gcagcggggc gacgtcttca cctgctccgt gatgcacgag gccttgcaca
     2641 accactacac gcagaagtcc atctcccgct ctccgggtaa atgagctagc tggccagaca
     2701 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     2761 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     2821 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     2881 ttttttaaag caagtaaaac ctctacaaat gtggtatgga attaattcta aaatacagca
     2941 tagcaaaact ttaacctcca aatcaagcct ctacttgaat ccttttctga gggatgaata
     3001 aggcataggc atcaggggct gttgccaatg tgcattagct gtttgcagcc tcaccttctt
     3061 tcatggagtt taagatatag tgtattttcc caaggtttga actagctctt catttcttta
     3121 tgttttaaat gcactgacct cccacattcc ctttttagta aaatattcag aaataattta
     3181 aatacatcat tgcaatgaaa ataaatgttt tttattaggc agaatccaga tgctcaaggc
     3241 ccttcataat atcccccagt ttagtagttg gacttaggga acaaaggaac ctttaataga
     3301 aattggacag caagaaagcg agcttctagc ttatcctcag tcctgctcct ctgccacaaa
     3361 gtgcacgcag ttgccggccg ggtcgcgcag ggcgaactcc cgcccccacg gctgctcgcc
     3421 gatctcggtc atggccggcc cggaggcgtc ccggaagttc gtggacacga cctccgacca
     3481 ctcggcgtac agctcgtcca ggccgcgcac ccacacccag gccagggtgt tgtccggcac
     3541 cacctggtcc tggaccgcgc tgatgaacag ggtcacgtcg tcccggacca caccggcgaa
     3601 gtcgtcctcc acgaagtccc gggagaaccc gagccggtcg gtccagaact cgaccgctcc
     3661 ggcgacgtcg cgcgcggtga gcaccggaac ggcactggtc aacttggcca tgatggctcc
     3721 tcctgtcagg agaggaaaga gaagaaggtt agtacaattg ctatagtgag ttgtattata
     3781 ctatgcagat atactatgcc aatgattaat tgtcaaacta gggctgcagg gttcatagtg
     3841 ccacttttcc tgcactgccc catctcctgc ccaccctttc ccaggcatag acagtcagtg
     3901 acttaccaaa ctcacaggag ggagaaggca gaagcttgag acagacccgc gggaccgccg
     3961 aactgcgagg ggacgtggct agggcggctt cttttatggt gcgccggccc tcggaggcag
     4021 ggcgctcggg gaggcctagc ggccaatctg cggtggcagg aggcggggcc gaaggccgtg
     4081 cctgaccaat ccggagcaca taggagtctc agccccccgc cccaaagcaa ggggaagtca
     4141 cgcgcctgta gcgccagcgt gttgtgaaat gggggcttgg gggggttggg gccctgacta
     4201 gtcaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa
     4261 accgctatcc acgcccattg atgtactgcc aaaaccgcat catcatggta atagcgatga
     4321 ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat
     4381 gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca
     4441 cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga
     4501 aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt
     4561 cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcct gcaggttaat
     4621 taagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4681 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4741 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4801 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4861 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4921 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4981 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5041 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5101 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     5161 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5221 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5281 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     5341 tttggtcatg gctagttaat taacatttaa atcagcggcc gcaataaaat atctttattt
     5401 tcattacatc tgtgtgttgg ttttttgtgt gaatcgtaac taacatacgc tctccatcaa
     5461 aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc
     5521 agaacatttc tctatcgaa
//
