LOCUS       sequence-302026-        6395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse synapsin-1 (mSyn1) promoter-mediates gene expression in 
            retinal neurons with fluorescent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Wang Q, Zhuang PS, Huang H, Li L, Liu L, Webber HC, Dalal R, Siew L,
            Fligor CM, Chang KC, Nahmou M, Kreymerman A, Sun Y, Meyer JS, 
            Goldberg JL, Hu Y
  TITLE     Mouse gamma-Synuclein Promoter-Mediated Gene Expression and Editing 
            in Mammalian Retinal Ganglion Cells.
  JOURNAL   J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020.
  PUBMED    32300046
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020."
COMMENT     Sequence Label: pAM-AAV-mSyn1-EGFP
FEATURES             Location/Qualifiers
     source          1..6395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        100..118
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     100..117
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     repeat_region   139..268
                     /label=AAV2 ITR
     CDS             join(1576..1578,1579..1581,1582..2295)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1576..1578,1579..1581,1582..2292)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1621..1642)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1882..1901)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2229..2250
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2353..2941
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2406..2426)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2824..2835)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2968..2985)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2974..3198
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(3408..3737)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3422..3557
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3722..3742
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(3983..4571)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4063..4082)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4742..5533,5534..5602))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5365..5384
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5603..5707)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5775..5793
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5831..5853)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5953..5972
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6186..6205
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ctgcttcagt aagccagatg ctacacaatt aggcttgtac atattgtcgt tagaacgcgg
       61 ctacaattaa tacataacct tatgtatcat acacatacga tttaggtgac actatagaat
      121 acacggaatt aattctagct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
      181 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      241 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      301 tctacgtagc catgctctag gtaccattga cgtcaataat gacgtatgtt cccatagtaa
      361 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      421 tggcagtaca tcaagtgtat caccatatgt gagccttctt attcagacca ccaaaattac
      481 tttatttttc cacatgaaag tattatgtgg cttcctgtct gcaaagagga agacatccat
      541 gaacactaat gacactgggt ttgggctatg tccggagcag aggaatgagg ccatgtagac
      601 taaatatgtg catgtggagg aggctgaaaa cacatcagag ctagcgctgc aggaaatgct
      661 tctgcattgc atacccagag tttccttgct catctgggag tctgtgtttt tcctagatgt
      721 gtgcacttgt gtgagattct ctgggtgtga gtcaaagtgt tatctgaatg tgtaatgtgt
      781 gctcaatatg ctcatgtgtg ttaccctgag cttctgtgtc tacatatata cctggatgcc
      841 tgtgtgttct gtgatgtaca tatatattct gtctttcctt ccttttctat ttgtgttatt
      901 ccatgtgttc tttcagattc tcaccaccaa gggcaaggat atgttaacta cccaagtgtc
      961 cacctccgcc tgtctggtga tgtttacgcc acccccgtgc tcttttcttt gcccgacaga
     1021 gttgttatag gagatgtctc cccgggaaca ctgcaggaag gagaatttct acatttatgt
     1081 tcccctctga gtgtgcttct atccccaaaa tgccttcaaa ggtgaaaatc aacactggaa
     1141 acccaagtat ctgggaaggg caagagtgtg taagtgcaag ttagcctaag gaataggaag
     1201 aggttggtaa acagggtagg atcgtgggag ggagtttcgt tactacaggt ccggaccctc
     1261 aggacaagaa ccccaccccc actccccaaa ttgcgcatcc cccgccccca tcagaggggg
     1321 aggggaagag gttgcggcgc ggcgcatgcg cactgtcgga ttcagcaccg cggtcagagc
     1381 cttcgcctcc gctgccggcg cgcaccacca cctccccagc accaaaggct gactgacgtc
     1441 actcactagc cctccccaaa ctccccttcc tcgccgcctt ggtcgcgtcc atgctgccgt
     1501 gagtccagtc ggaccgcacc acgagaggtg caagataggg aggtgcgggc gcgaccatac
     1561 gctctgcggg aattcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     1621 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     1681 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     1741 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     1801 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     1861 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     1921 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     1981 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     2041 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     2101 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     2161 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     2221 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     2281 gagctgtaca agtaaagatc tacgcgtggt acctctagag tcgacccggg cggcctcgag
     2341 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     2401 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     2461 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     2521 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     2581 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     2641 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     2701 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     2761 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     2821 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     2881 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     2941 catcgatacc gtcgactcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     3001 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     3061 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     3121 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     3181 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg actagagcat
     3241 ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc cctagtgatg
     3301 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     3361 gcccgacgcc cgggcggcct cagtgagcga gcgagcgcgc agagcttttt gcaaaagcct
     3421 aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg
     3481 gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc
     3541 ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga
     3601 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca cctggttgct
     3661 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
     3721 caccctaact gacacacatt ccacagctgc attaatgaat cggccaacgc gcggggagag
     3781 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     3841 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3901 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3961 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4021 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4081 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     4141 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     4201 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4261 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4321 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4381 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     4441 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4501 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4561 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4621 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     4681 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     4741 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     4801 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     4861 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     4921 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     4981 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     5041 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     5101 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     5161 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     5221 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     5281 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     5341 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     5401 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     5461 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     5521 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     5581 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     5641 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     5701 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     5761 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     5821 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     5881 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     5941 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccattcg acgctctccc
     6001 ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc
     6061 gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct
     6121 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
     6181 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
     6241 gccacgatgc gtccggcgta gaggatctgg ctagcgatga ccctgctgat tggttcgctg
     6301 accatttccg ggtgcgggac ggcgttacca gaaactcaga aggttcgtcc aaccaaaccg
     6361 actctgacgg cagtttacga gagagatgat agggt
//
