LOCUS       sequence-302028-        6116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG promoter-mediates gene expression in retinal neurons with 
            fluorescent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Wang Q, Zhuang PS, Huang H, Li L, Liu L, Webber HC, Dalal R, Siew L,
            Fligor CM, Chang KC, Nahmou M, Kreymerman A, Sun Y, Meyer JS, 
            Goldberg JL, Hu Y
  TITLE     Mouse gamma-Synuclein Promoter-Mediated Gene Expression and Editing 
            in Mammalian Retinal Ganglion Cells.
  JOURNAL   J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020.
  PUBMED    32300046
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020."
COMMENT     Sequence Label: pAM-AAV-CAG-EGFP
FEATURES             Location/Qualifiers
     source          1..6116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        100..118
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     100..117
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     repeat_region   139..268
                     /label=AAV2 ITR
     promoter        574..851
                     /label=chicken beta-actin promoter
     primer_bind     1130..1152
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1214..1233
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(1297..1299,1300..1302,1303..2016)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1297..1299,1300..1302,1303..2013)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1342..1363)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1603..1622)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1950..1971
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2074..2662
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2127..2147)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2545..2556)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2689..2706)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2695..2919
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(3129..3458)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3143..3278
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3443..3463
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(3704..4292)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3784..3803)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4463..5254,5255..5323))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5086..5105
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5324..5428)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5496..5514
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5552..5574)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5674..5693
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5907..5926
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ctgcttcagt aagccagatg ctacacaatt aggcttgtac atattgtcgt tagaacgcgg
       61 ctacaattaa tacataacct tatgtatcat acacatacga tttaggtgac actatagaat
      121 acacggaatt aattctagct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
      181 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      241 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      301 tctacgtagc catgctctag gtaccattga cgtcaataat gacgtatgtt cccatagtaa
      361 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      421 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      481 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      541 acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca
      601 ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat
      661 tttgtgcagc gatgggggcg gggggggggg gggggcgcgc gccggggggg gggggggggg
      721 gsggggggsg gggsggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc
      781 tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg
      841 cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct
      901 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
      961 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt
     1021 ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg
     1081 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     1141 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     1201 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg
     1261 gatccaccgg tgcggccgca cgcgtctcga gaattcatgg tgagcaaggg cgaggagctg
     1321 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1381 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1441 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     1501 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1561 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1621 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1681 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     1741 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     1801 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     1861 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     1921 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     1981 gggatcactc tcggcatgga cgagctgtac aagtaaagat ctacgcgtgg tacctctaga
     2041 gtcgacccgg gcggcctcga gaagcttatc gataatcaac ctctggatta caaaatttgt
     2101 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     2161 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     2221 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     2281 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     2341 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     2401 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     2461 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     2521 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     2581 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     2641 tccctttggg ccgcctcccc gcatcgatac cgtcgactcg ctgatcagcc tcgactgtgc
     2701 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     2761 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     2821 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     2881 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     2941 gctggggctc gactagagca tggctacgta gataagtagc atggcgggtt aatcattaac
     3001 tacaaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
     3061 gaggccgggc gaccaaaggt cgcccgacgc ccgggcggcc tcagtgagcg agcgagcgcg
     3121 cagagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc
     3181 tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc
     3241 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact
     3301 atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     3361 gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     3421 ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg cattaatgaa
     3481 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     3541 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     3601 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     3661 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3721 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3781 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3841 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3901 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3961 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4021 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4081 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4141 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4201 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4261 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     4321 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4381 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4441 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     4501 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     4561 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     4621 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     4681 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     4741 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     4801 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     4861 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     4921 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     4981 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     5041 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     5101 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     5161 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     5221 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     5281 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     5341 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     5401 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     5461 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     5521 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     5581 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     5641 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     5701 tgcaccattc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
     5761 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
     5821 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
     5881 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
     5941 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatctg gctagcgatg
     6001 accctgctga ttggttcgct gaccatttcc gggtgcggga cggcgttacc agaaactcag
     6061 aaggttcgtc caaccaaacc gactctgacg gcagtttacg agagagatga tagggt
//
