LOCUS       sequence-30203-e         661 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Morimoto-Tomita M, Uchimura K, Bistrup A, Lum DH, Egeblad M, 
            Boudreau N, Werb Z, Rosen SD
  TITLE     Sulf-2, a proangiogenic heparan sulfate endosulfatase, is 
            upregulated in breast cancer.
  JOURNAL   Neoplasia. 2005 Nov . 7(11):1001-10.
  PUBMED    16331886
REFERENCE   2  (bases 1 to 661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neoplasia. 
            2005 Nov . 7(11):1001-10."
COMMENT     Sequence Label: f1H (264)
FEATURES             Location/Qualifiers
     source          1..661
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     92..151
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             212..235
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagccac catggagaca gacacactcc tgctatgggt
      121 actgctgctc tgggttccag gttccactgg tgacgcggcc cagccggcca ggcgcgcgcg
      181 ccgtacgaag cttggtaccg agctcggatc cgattacaag gatgacgacg ataagccagc
      241 agcatcgact gtcagatccc cgaggttcag aggacggata cagcaggaac gaaaaaacat
      301 ccgacccaac attattcttg tgcttaccga tgatcaagat gtggagctgg ggtccctgca
      361 agtcatgaac aaaacgagaa agattatgga acatgggggg gccaccttca tcaatgcctt
      421 tgtgactaca cccatgtgct gcccgtcacg gtcctccatg ctcaccggga agtatgtgca
      481 caatcacaat gtctacacca acaacgagaa ctgctcttcc ccctcgtggc aggccatgca
      541 tgagcctcgg acttttgctg tatatcttaa caacactggc tacagaacag ccttttttgg
      601 aaaatacctc aatgaatata atggcagcta catcccccct gggtggcgag aatggcttgg
      661 a
//
