LOCUS       sequence-302033-        2470 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Heme Oxygenase (Nostocaceae) and ReBphP-PCM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Mishra K, Stankevych M, Fuenzalida-Werner JP, Grassmann S, Gujrati 
            V, Huang Y, Klemm U, Buchholz VR, Ntziachristos V, Stiel AC
  TITLE     Multiplexed whole-animal imaging with reversibly switchable 
            optoacoustic proteins.
  JOURNAL   Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi: 10.1126/sciadv.aaz6293. 
            eCollection 2020 Jun.
  PUBMED    32582850
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-06-12"; volume: "6(24):eaaz6293. doi"; pages: " 
            10.1126/sciadv.aaz6293. eCollection 2020 Jun"
COMMENT     Sequence Label: pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM)
FEATURES             Location/Qualifiers
     source          1..2470
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     813..832
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        813..831
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    832..856
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ccatggatgg cgatgagcag caatttagca aacaaactac gtgtaggtac gaaaaaagcc
       61 cacacaatgg cagaaaatgt aggttttgtc aaatgcttct taaaaggagt ggtagaaaaa
      121 agctcttacc ggaagctagt ggccaacttc tactacgtct actccgcaat ggaagaggag
      181 atggaaaagc atagccagca cccaattgtt tctaaaataa acttttccca actgaatcgt
      241 aagcagactc tagagcaaga cctgagttac tattacggcg ctaactggcg ggaacaaatt
      301 caactgtcgc cagcaggtga agcttacgta caacgcattc gggaaatctc tgcaaccgaa
      361 ccagaattgt tgattgccca ctcttacacc cgttatttag gtgatttatc cgggggacaa
      421 attctcaaaa acattgctgt gacagcgatg aatttgaatg atgggcaagg aactgcattt
      481 tatgagttcg cagatatttc tgatgagaaa gcttttaagg ctaaatatcg tcaaacatta
      541 gatgagttgg caattgatga agcgacaggc gatcgcattg ttgatgaagc taacgccgct
      601 tttggtatga acatgaaaat gttccaagaa ctagaaggga acctcatcaa ggcaatcggc
      661 atgatgctgt tcaacacctt gactcgcaag cgcacacgcg gcgctgccga actagccact
      721 gctgagtaac tgcaggtcga caagcttgcg gccgcataat gcttaagtcg aacagaaagt
      781 aatcgtattg tacacggccg cataatcgaa attaatacga ctcactatag gggaattgtg
      841 agcggataac aattccccat cttagtatat tagttaagta taagaaggag atatacatat
      901 gagcggcacc agagaaccca tggacctgac caactgcgac agagagccta tccatcagct
      961 gggcgctatc cagcctttcg gcttcctgct gcaagtgtcc agcgactgga tcgtgacaag
     1021 agcctccgtg aacctggccg agtttctggg agtgacacag gccgatgctc tgggcagacc
     1081 tgccagcaca ctgatcatgc ctgaggctct gcacaccatc cggaacaagc tgaccacact
     1141 gagaggcgcc gacgtggtgg aaagagtgtt cgccattgct ctgacccctg accagagcaa
     1201 gttcgacgtg gccgtgcact tcaacgagag ccaagtgatc atcgagggcg agagatgcca
     1261 agaggacaga agaaacgccc ctagcctgag catgcggagc atgatgagca gactggacca
     1321 ggccgagaca cttgaggcct ttttcagaga gggcgccaga caggctagag ccctgaccgg
     1381 attcgacaga gtgatggtgt acagattcga cgaaggcggc tctggcgaag tggtggctga
     1441 agctgctaga agcggcatcg gcagctttct gggcctgcac tatcctgcca gcgacatccc
     1501 tgtgcaggcc agagcactgt acctgcggaa cctgttcaga atcattgccg atgtggacgc
     1561 cgtgcctgtg ccaattctgc ccgagagaga tgagcacggc cagcctctgg atctgagcat
     1621 gagcgtgctg agaagcgtgt cccctatcca catcgagtac ctgaagaaca tgggcgtggg
     1681 cgccagcctg tctatcagca ttgtggtgga tggcaagctg tggggcctgt tcgcctgtca
     1741 ccactacgaa cctagactgc ccagcgctca gagcagatct accgccgaac tgtttggcca
     1801 gatgttcgcc agcaggctgg aaagcagaga gagaaggctg gccctggact acgagacaaa
     1861 ggccagaaga atcgccgacc ggctgctgac atccgtggcc gataatgcct ctctgctgga
     1921 cgatcctgcc tggctgattg aagccctggc cgatgccatt cctgccgatg gaatcggcgt
     1981 gtggatcaac ggtagactgg ctctggccgg catcggaccc gacaagaaga attttgccag
     2041 cctcgtgcgg cacctgaaca gaaatgctgg cggcagaatc tacgccgtgg acagactgag
     2101 ccagacatac cccgacctgg aaatcgatgc tgtggtggcc ggaatgctgg ctatccccat
     2161 cagcagatcc ccacgggatt acgtggtgct gttccggcaa gaactcgtgc ggacagttag
     2221 atggggcgga gatcctcaca agcccgtgga atacggccct aatggcccta gactgacccc
     2281 tcggaagtcc ttcgaagagt ggagcgaact tgtgcggggc agaagcctgc cttttaccga
     2341 ggctgaacag agagtggccg aaaccatcag agtgaccctg atcgaggtgg tgctgagact
     2401 gacagacgag gcctctatgg ccagacagat ggccaatgag agacaagagc tgctgatcgc
     2461 cgagctgaac
//
