LOCUS       sequence-302054-        6570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse Camk2a (mCamk2a) promoter-mediates gene expression in retinal 
            neurons with fluorescent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Wang Q, Zhuang PS, Huang H, Li L, Liu L, Webber HC, Dalal R, Siew L,
            Fligor CM, Chang KC, Nahmou M, Kreymerman A, Sun Y, Meyer JS, 
            Goldberg JL, Hu Y
  TITLE     Mouse gamma-Synuclein Promoter-Mediated Gene Expression and Editing 
            in Mammalian Retinal Ganglion Cells.
  JOURNAL   J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020.
  PUBMED    32300046
REFERENCE   2  (bases 1 to 6570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020."
COMMENT     Sequence Label: pAM-AAV-mCamk2a-EGFP
FEATURES             Location/Qualifiers
     source          1..6570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        300..1588
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     CDS             join(1596..1598,1599..1601,1602..2315)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1596..1598,1599..1601,1602..2312)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1641..1662)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1902..1921)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2249..2270
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2373..2961
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2426..2446)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2844..2855)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2988..3005)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2994..3218
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(3428..3757)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3442..3577
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3742..3762
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(4003..4591)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4083..4102)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4762..5553,5554..5622))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5385..5404
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5623..5727)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5795..5813
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5851..5873)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5973..5992
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6206..6225
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        6515..6533
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     6515..6532
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     repeat_region   join(6554..6570,1..113)
                     /label=AAV2 ITR
ORIGIN
        1 cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc tttggtcgcc cggcctcagt
       61 gagcgagcga gcgcgcagag agggagtggc caactccatc actaggggtt ccttgtagtt
      121 aatgattaac ccgccatgct acttatctac gtagccatgc tctaggtacc attgacgtca
      181 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      241 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcataa ttcctatgcc
      301 attatggcct taggtcactt catctccatg gggttcttct tctgattttc tagaaaatga
      361 gatgggggtg cagagagctt cctcagtgac ctgcccaggg tcacatcaga aatgtcagag
      421 ctagaacttg aactcagatt actaatctta aattccatgc cttgggggca tgcaagtacg
      481 atatacagaa ggagtgaact cattagggca gatgaccaat gagtttagga aagaagagtc
      541 cagggcaggg tacatctaca ccacccgccc agccctgggt gagtccagcc acgttcacct
      601 cattatagtt gcctctctcc agtcctacct tgacgggaag cacaagcaga aactgggaca
      661 ggagccccag gagaccaaat cttcatggtc cctctgggag gatgggtggg gagagctgtg
      721 gcagaggcct caggaggggc cctgctgctc agtggtgaca gataggggtg agaaagcaga
      781 cagagtcatt ccgtcagcat tctgggtctg tttggtactt cttctcacga taaggtggcg
      841 gtgtgatatg cacaatggct aaaaagcagg gagagctgga aagaaacaag gacagagaca
      901 gaggccaagt caaccagacc aattcccaga ggaagcaaag aaaccattac agagactaca
      961 agggggaagg gaaggagaga tgaattagct tcccctgtaa accttagaac ccagctgttg
     1021 ccagggcaac ggggcaatac ctgtctcttc agaggagatg aagttgccag ggtaactaca
     1081 tcctgtcttt ctcaaggacc atcccagaat gtggcaccca ctagccgtta ccatagcaac
     1141 tgcctctttg ccccacttaa tcccatcccg tctgttaaaa gggccctata gttggaggtg
     1201 ggggaggtag gaagagcgat gatcacttgt ggactaagtt tgttcgcatc cccttctcca
     1261 accccctcag tacatcaccc tgggggaaca gggtccactt gctcctgggc ccacacagtc
     1321 ctgcagtatt gtgtatataa ggccagggca aagaggagca ggttttaaag tgaaaggcag
     1381 gcaggtgttg gggaggcagt taccggggca acgggaacag ggcgtttcgg aggtggttgc
     1441 catggggacc tggatgctga cgaaggctcg cgaggctgtg agcagccaca gtgccctgct
     1501 cagaagcccc aagctcgtca gtcaagccgg ttctccgttt gcactcagga gcacgggcag
     1561 gcgagtggcc cctagttctg ggggcagcag aattcatggt gagcaagggc gaggagctgt
     1621 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     1681 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     1741 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     1801 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     1861 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     1921 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     1981 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     2041 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     2101 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     2161 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     2221 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     2281 ggatcactct cggcatggac gagctgtaca agtaaagatc tacgcgtggt acctctagag
     2341 tcgacccggg cggcctcgag aagcttatcg ataatcaacc tctggattac aaaatttgtg
     2401 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     2461 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     2521 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     2581 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     2641 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     2701 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     2761 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     2821 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     2881 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     2941 ccctttgggc cgcctccccg catcgatacc gtcgactcgc tgatcagcct cgactgtgcc
     3001 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     3061 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     3121 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     3181 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     3241 ctggggctcg actagagcat ggctacgtag ataagtagca tggcgggtta atcattaact
     3301 acaaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     3361 aggccgggcg accaaaggtc gcccgacgcc cgggcggcct cagtgagcga gcgagcgcgc
     3421 agagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     3481 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     3541 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     3601 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
     3661 actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     3721 gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgc attaatgaat
     3781 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     3841 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     3901 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     3961 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4021 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4081 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4141 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4201 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4261 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4321 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4381 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4441 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4501 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4561 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4621 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     4681 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     4741 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     4801 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     4861 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     4921 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     4981 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     5041 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     5101 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     5161 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     5221 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     5281 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     5341 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     5401 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     5461 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     5521 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     5581 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     5641 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     5701 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     5761 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     5821 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     5881 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     5941 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     6001 gcaccattcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
     6061 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca
     6121 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
     6181 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
     6241 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctgg ctagcgatga
     6301 ccctgctgat tggttcgctg accatttccg ggtgcgggac ggcgttacca gaaactcaga
     6361 aggttcgtcc aaccaaaccg actctgacgg cagtttacga gagagatgat agggtctgct
     6421 tcagtaagcc agatgctaca caattaggct tgtacatatt gtcgttagaa cgcggctaca
     6481 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaatacacg
     6541 gaattaattc tagctgcgcg ctcgctcgct
//
