LOCUS       sequence-302056-        6621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human gamma-synuclein (hSncg) promoter-mediates gene expression in 
            retinal neurons with fluorescent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Wang Q, Zhuang PS, Huang H, Li L, Liu L, Webber HC, Dalal R, Siew L,
            Fligor CM, Chang KC, Nahmou M, Kreymerman A, Sun Y, Meyer JS, 
            Goldberg JL, Hu Y
  TITLE     Mouse gamma-Synuclein Promoter-Mediated Gene Expression and Editing 
            in Mammalian Retinal Ganglion Cells.
  JOURNAL   J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020.
  PUBMED    32300046
REFERENCE   2  (bases 1 to 6621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020."
COMMENT     Sequence Label: pAM-AAV-hSncg-EGFP
FEATURES             Location/Qualifiers
     source          1..6621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        175..193
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     175..192
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     repeat_region   214..343
                     /label=AAV2 ITR
     CDS             join(1877..1879,1880..1882,1883..2596)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1877..1879,1880..1882,1883..2593)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1922..1943)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2183..2202)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2530..2551
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2654..3242
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2707..2727)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3125..3136)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3269..3286)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3275..3499
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(3709..4038)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3723..3858
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4023..4043
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(4284..4872)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4364..4383)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5043..5834,5835..5903))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5666..5685
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5904..6008)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6076..6094
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6132..6154)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6254..6273
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6487..6506
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ggcgttacca gaaactcaga aggttcgtcc aaccaaaccg actctgacgg cagtttacga
       61 gagagatgat agggtctgct tcagtaagcc agatgctaca caattaggct tgtacatatt
      121 gtcgttagaa cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag
      181 gtgacactat agaatacacg gaattaattc tagctgcgcg ctcgctcgct cactgaggcc
      241 gcccgggcaa agcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga
      301 gcgcgcagag agggagtggc caactccatc actaggggtt ccttgtagtt aatgattaac
      361 ccgccatgct acttatctac gtagccatgc tctaggtacc attgacgtca ataatgacgt
      421 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      481 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaaggtcc aggccctctt
      541 accgtccaac ggggtccttg ctggtgtcct cagcctctgc cttccagacc ccaggtgtcc
      601 tggagctctg cagatcagag aggctagtac tggaagcctg ggcccatgcc cccagcagcc
      661 cccagcccag ggggccccca aggctgaaca gcaagctcag gatcatcttg gtggtggggc
      721 aggctcagct cacactcagc cttggcaagt agctccagaa actgctagtg acgttgtctt
      781 caagttaaat ctcaggagga aaagaaaata cgaggacaac aaagagagga agtggcctgg
      841 gccggcctac ccggtgggtc ttgtcctgcc ccccaactac cctggctggc cccacagggg
      901 ccgccaacca cacaagccag ttcctgtccc tgaggacttg gctcagggac tctgggaatg
      961 tggtagacat ggggtggccc caccaaatgc atccttatgg gaacctgctc cctgggagcc
     1021 atgaaaagag cgtggacttc gaggtggggc cacaggaagt ggtcaggtcc atctcagggg
     1081 acctgctgcc catccacact gctggccagg aaatgggggg caattcatgc ctcctcagca
     1141 ccttcagcac tgggcggctc aaagaaggca agggactatt ctggggtcac acagcatgca
     1201 gccagaggcc aaggcatgag gaagtccttc atttccccac ccccacccac ctcagatcct
     1261 ccaaccggtt tcatggcagc ccagggtcca gcggcatcca ggatgctggt gggtagctgc
     1321 acagcccagg ccgcgggagg ttggctgctc tcacctaaca ggcctatgtg gccctgaccc
     1381 ctacctagga agctggggac aatggccaag gcgcctcccc tctctgtgcc tgtctgtcca
     1441 ggtgcagcat agacacagca cccctggggc caagagcacc cagccagggc tgcccccatg
     1501 ggtgggcagg gcagtaaatg aatgagggac aggttgggag gtggccagcc ccctccagcc
     1561 catggagggc acggggcagg agagctgggc tgagccagca ggagcccagg gagcctggtc
     1621 tctgccttcc tatcctggag gaaggtgagg ctgaacctcc ttccctccct ccctccctcc
     1681 ccgcccccac tgcacgcagg gctggctggg ctccagctgg cctccgcatc aatatttcat
     1741 cggcgtcaat aggaggcatc ggggacagcc gctgcggcag cactcgagcc agctcaagcc
     1801 cgcagctcgc agggagatcc agctccgtcc tgcctgcagc agcccaaccc tgcacaccca
     1861 ccatggatgt gaattcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     1921 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     1981 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     2041 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     2101 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     2161 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     2221 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     2281 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     2341 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     2401 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     2461 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     2521 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     2581 cgagctgtac aagtaaagat ctacgcgtgg tacctctaga gtcgacccgg gcggcctcga
     2641 gaagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     2701 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     2761 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     2821 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     2881 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     2941 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     3001 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     3061 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     3121 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     3181 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     3241 gcatcgatac cgtcgactcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     3301 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     3361 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     3421 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     3481 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc gactagagca
     3541 tggctacgta gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat
     3601 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt
     3661 cgcccgacgc ccgggcggcc tcagtgagcg agcgagcgcg cagagctttt tgcaaaagcc
     3721 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc
     3781 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     3841 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
     3901 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc
     3961 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     4021 acaccctaac tgacacacat tccacagctg cattaatgaa tcggccaacg cgcggggaga
     4081 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4141 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4201 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4261 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4321 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4381 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4441 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4501 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4561 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4621 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4681 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     4741 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4801 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4861 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4921 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4981 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     5041 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     5101 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     5161 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     5221 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     5281 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     5341 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     5401 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5461 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5521 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5581 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5641 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     5701 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     5761 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     5821 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     5881 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     5941 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     6001 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     6061 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     6121 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     6181 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     6241 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccattc gacgctctcc
     6301 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     6361 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     6421 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     6481 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     6541 ggccacgatg cgtccggcgt agaggatctg gctagcgatg accctgctga ttggttcgct
     6601 gaccatttcc gggtgcggga c
//
