LOCUS       sequence-302059-        7056 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse gamma-synuclein (mSncg) promoter-mediates Cre expression in 
            retinal ganglion cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Wang Q, Zhuang PS, Huang H, Li L, Liu L, Webber HC, Dalal R, Siew L,
            Fligor CM, Chang KC, Nahmou M, Kreymerman A, Sun Y, Meyer JS, 
            Goldberg JL, Hu Y
  TITLE     Mouse gamma-Synuclein Promoter-Mediated Gene Expression and Editing 
            in Mammalian Retinal Ganglion Cells.
  JOURNAL   J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020.
  PUBMED    32300046
REFERENCE   2  (bases 1 to 7056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020."
COMMENT     Sequence Label: pAM-AAV-mSncg-Cre
FEATURES             Location/Qualifiers
     source          1..7056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   38..167
                     /label=AAV2 ITR
     CDS             1806..1826
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1827..2855
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    2913..3501
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2966..2986)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3384..3395)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3528..3545)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3534..3758
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(3968..4297)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3982..4117
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4282..4302
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(4543..5131)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4623..4642)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5302..6093,6094..6162))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5925..5944
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6163..6267)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6335..6353
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6391..6413)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6513..6532
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6746..6765
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(7055..7056,1..17)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     join(7055..7056,1..16)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ttaggtgaca ctatagaata cacggaatta attctagctg cgcgctcgct cgctcactga
       61 ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt cgcccggcct cagtgagcga
      121 gcgagcgcgc agagagggag tggccaactc catcactagg ggttccttgt agttaatgat
      181 taacccgcca tgctacttat ctacgtagcc atgctctagg taccattgac gtcaataatg
      241 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      301 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatggtccc atgccactag
      361 tgggagctgt gttacctgtt gcagccccac ccaaagcccc tgctataggt caagcaggaa
      421 tcaccctgcc atccccagcc tggggcctgg agtaccagat ccaggaaact agcatccctt
      481 agctatagag atagccacac atcagcccat tcctcagatg tgtatctggg gctcagacat
      541 catctcccga tctccgacaa gggcaggatt tccttaccgt ctgatggggt ctctgctggt
      601 atcctcagcc cctagtctcc agccttcagg catgccgggc ctattgagat gggagaactt
      661 ggtaccgggg tcctgtgccc aggaccctag cagtccccag ctcaggtaca ccccaaagcc
      721 cagcagcagt gtcgggatca tggtgagggg ctcctgtgct gatgctcagc cttacaagtg
      781 actctcaaat ttgctggtga tgtggtcttc aagcgaaatg tcagaaagaa aagaaaacac
      841 gaggacaaca aagggaggaa gtggcctggt ccggcccacc cggcaagtct catccgcccc
      901 cgcccccgcc ccttccagcc tggccccctt ggaggcctcc aaccactcag gtcaattcct
      961 gtgtcctgag ggcacttgaa tcagggacac gggatttggt agacacataa aggtggcccc
     1021 attaaactta tttctccagg actctgtcgt gggcctggag gagatctggt gccacccata
     1081 ctgttggcca ggaagtgggg aacgggcaca tcacacctgc tcggcacctt gggctatggg
     1141 aactagcagg tgggtgggaa ctcagagaag gaaagggact atgctagaat cacacagcgg
     1201 gcagcccagt ctagggcatg gggagcagct ttgggtgttt ctggcctcag ccttccaaca
     1261 ggtttggcta gagctccagg ctcaagagca tccaggatac agtggggaac tggataacag
     1321 ggcagcctgc aggttggcca ttcattggat tggccctgac cccggcccag cctgggacac
     1381 tgaggcatca tcagtcaagg cacttttctt ctgcatataa gagccagggc acgagaccac
     1441 cagggctttc caaggatgaa tgaggtgtaa tgatagatta ggatatgtcc agcctccaac
     1501 acgctctccc tcccccaggg ccaacaagag tcagcagggc agaatagagc cagtaggggc
     1561 ccgggccctg ctcgctggta tccccgtgag gcatgccttc tctctggccc gccctccctg
     1621 cccccaccct ggcccgggct ggctgggctc cagccagcag ccacagcatc aatatttcat
     1681 ctgcgtcaat aagaggcagt agcagcagag acagcggctg cggcagcact ccagtccata
     1741 gcttgcagca gccaggttcc atccttgcaa acaccatgga cgtcttcaag gaattcgcca
     1801 ccatgcccaa gaagaagagg aaggtgtcca atttactgac cgtacaccaa aatttgcctg
     1861 cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa cctgatggac atgttcaggg
     1921 atcgccaggc gttttctgag catacctgga aaatgcttct gtccgtttgc cggtcgtggg
     1981 cggcatggtg caagttgaat aaccggaaat ggtttcccgc agaacctgaa gatgttcgcg
     2041 attatcttct atatcttcag gcgcgcggtc tggcagtaaa aactatccag caacatttgg
     2101 gccagctaaa catgcttcat cgtcggtccg ggctgccacg accaagtgac agcaatgctg
     2161 tttcactggt tatgcggcgg atccgaaaag aaaacgttga tgccggtgaa cgtgcaaaac
     2221 aggctctagc gttcgaacgc actgatttcg accaggttcg ttcactcatg gaaaatagcg
     2281 atcgctgcca ggatatacgt aatctggcat ttctggggat tgcttataac accctgttac
     2341 gtatagccga aattgccagg atcagggtta aagatatctc acgtactgac ggtgggagaa
     2401 tgttaatcca tattggcaga acgaaaacgc tggttagcac cgcaggtgta gagaaggcac
     2461 ttagcctggg ggtaactaaa ctggtcgagc gatggatttc cgtctctggt gtagctgatg
     2521 atccgaataa ctacctgttt tgccgggtca gaaaaaatgg tgttgccgcg ccatctgcca
     2581 ccagccagct atcaactcgc gccctggaag ggatttttga agcaactcat cgattgattt
     2641 acggcgctaa ggatgactct ggtcagagat acctggcctg gtctggacac agtgcccgtg
     2701 tcggagccgc gcgagatatg gcccgcgctg gagtttcaat accggagatc atgcaagctg
     2761 gtggctggac caatgtaaat attgtcatga actatatccg taacctggat agtgaaacag
     2821 gggcaatggt gcgcctgctg gaagatggcg attagagatc tacgcgtggt acctctagag
     2881 tcgacccggg cggcctcgag aagcttatcg ataatcaacc tctggattac aaaatttgtg
     2941 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     3001 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     3061 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     3121 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     3181 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     3241 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     3301 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     3361 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     3421 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     3481 ccctttgggc cgcctccccg catcgatacc gtcgactcgc tgatcagcct cgactgtgcc
     3541 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     3601 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     3661 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     3721 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     3781 ctggggctcg actagagcat ggctacgtag ataagtagca tggcgggtta atcattaact
     3841 acaaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     3901 aggccgggcg accaaaggtc gcccgacgcc cgggcggcct cagtgagcga gcgagcgcgc
     3961 agagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     4021 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     4081 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     4141 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
     4201 actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     4261 gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgc attaatgaat
     4321 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     4381 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4441 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4501 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4561 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4621 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4681 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4741 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4801 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4861 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4921 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4981 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5041 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5101 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     5161 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     5221 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     5281 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     5341 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     5401 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     5461 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     5521 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     5581 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     5641 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     5701 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     5761 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     5821 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     5881 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     5941 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     6001 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     6061 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     6121 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     6181 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     6241 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     6301 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     6361 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     6421 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     6481 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     6541 gcaccattcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
     6601 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca
     6661 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
     6721 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
     6781 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctgg ctagcgatga
     6841 ccctgctgat tggttcgctg accatttccg ggtgcgggac ggcgttacca gaaactcaga
     6901 aggttcgtcc aaccaaaccg actctgacgg cagtttacga gagagatgat agggtctgct
     6961 tcagtaagcc agatgctaca caattaggct tgtacatatt gtcgttagaa cgcggctaca
     7021 attaatacat aaccttatgt atcatacaca tacgat
//
