LOCUS       sequence-302063-        9451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of FseI endonuclease in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9451)
  AUTHORS   Gnugge R, Symington LS
  TITLE     Efficient DNA double-strand break formation at single or multiple 
            defined sites in the Saccharomyces cerevisiae genome.
  JOURNAL   Nucleic Acids Res. 2020 Oct 14. pii: 5923424. doi: 
            10.1093/nar/gkaa833.
  PUBMED    33053188
REFERENCE   2  (bases 1 to 9451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Oct 14. pii: 5923424. doi: 10.1093/nar/gkaa833."
COMMENT     Sequence Label: pRG659_lexO-FseI_LexA-TF_LEU2MX
FEATURES             Location/Qualifiers
     source          1..9451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2597..2619
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2611..2628
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2612..2628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2635..2654
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2635..2653
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3728..3748
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3755..3778
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      3828..4077
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             join(4747..4998,4999..5352)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     5290..5311
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             5371..6306
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             5419..6309
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     terminator      6773..7022
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(7185..7203)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7224..7240)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7224..7240)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7237..7259)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7248..7264
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(7523..7540)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7694..8282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7774..7793)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8461..9252,9253..9321))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9084..9103
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9322..9426)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcgaaa gaacgcggca ttagcaacat ttgcgaagcg aacgattttg cgaactgcta
       61 ttaattaact ctggcgcgcc taactttttg tgtggtgccc tcctccttgt caatattaat
      121 gttaaagtgc aattcttttt ccttatcacg ttgagccatt agtatcaatt tgcttacctg
      181 tattccttta catcctcctt tttctccttc ttgataaatg tatgtagatt gcgtatatag
      241 tttcgtctac cctatgaaca tattccattt tgtaatttcg tgtcgcatat gatccgcagg
      301 ctaaccggaa cctgtattat ttagtttatg ctacgttaaa taaagacctt tcgttcacat
      361 aactgaatgt gtaatggcct tgagatttca agcataccaa gttggtggag acggggtcgt
      421 tacaaaagac tctttaagcc aagatttcct tgacagcctt ggcgatagca tcgccaacct
      481 cagtggtaga gttagaacca ccaaggtcac cggttctgac acctgcatcc aagacatttc
      541 taacagcttc ttcaagagcc ctaccttctt caaccaaatc caaggataac ttcaacatca
      601 tagctgcaga taagatggta gcaattgggt taaccttgtt tgctggtaaa tctggggcag
      661 aaccatgaca tggttcgtac aaaccgaatg ccttgttagt gtcaggtagg gaagctagag
      721 atgcagaagg taataaaccc aaagaacctg gaataacaga ggcttcatcg gagataatat
      781 caccaaacat gttgttggta ataacaacac cgtttagctt agttggtgat ttaaccaaaa
      841 tcatagcagc agagtcgatc aattggtgct gaacagttaa ttgtgggaac tcagtcttga
      901 tggtttcttc aacagtcttt ctccacaatc tggaagaggc aagcacgtta gccttgtcaa
      961 gagaccagat tggtaatggt gggttttgtt gcaatgccaa gaaagcagcc attcttgtaa
     1021 ttctttgaac ttcaggaaca ctgtatttct cagagtccca agcaactccg tcaccttcat
     1081 cttcttttct ttcaccaaag tagataccac caaccaattc tctaacgacg acgaaatcag
     1141 taccctttgc atattcaggc ttcaaaggag aaagatctag taaagaatca gaagcaaagt
     1201 tacatggtct caagttggcg tatagaccca attcctttct gatcttcaat agaccttgtt
     1261 ctggtctaac tgcgcccgta ccccattttg gaccaccaac agcacctagt aagacagcat
     1321 cggctttctt agaggcttca agagcttcat ctggtaaagg agtgccagtg gcatcgatgg
     1381 cagcaccccc gatcaagtga tgttggaaat tgaacttaat ttctggacgg acttcagcaa
     1441 tggcattcaa gaccttaata gcttcgtcag taacttcttt accgacgtga tcacccggta
     1501 ggacaacgat attcttagac atggtataat ctgtgtagtg tgggatactt tttacttctt
     1561 caaataggta tcaacaataa aaataaatcg aagcaaatgt aggaatgcgt taaagcagat
     1621 gtacttactt agagtacata gatatattta tataattcaa tatataaaag tatatgaagc
     1681 atctgatgtt gaacctgtca tgactctaaa ctggaatggg tagttatgct tctgaaggtt
     1741 ttccgttaga aaatgagtgc agggatcagg cccggaaccg gttttagcct gaaaggaaaa
     1801 gaaaaaaatg cggtgattac tagcacgtga ctgcgctgaa ttggaatcta ccacaagtac
     1861 agcgaatggc aagagggaag gggaatatat tgtgcctggc ggtagcgtct gctatatcaa
     1921 atgttaaaca taactaaatg cagtagtgaa tagagtaatg tatttctaca tctgtgggta
     1981 gggccaatgg gagcccgatg tctattgtac agatattttc cttttatagt taatcaattc
     2041 aacttggaaa tcattgagtc gatgtctact tggataactg gagccgtggc agcgtttgaa
     2101 atttgacgag tcttcttgtt ttgttcgcgc attttcagtt tattcaaacg ggagatggca
     2161 ccgaatccac tgacttctct gttcaatttg aatggtaata ggaagaaagt acatttcgag
     2221 aaattttcgc ccaacgtcga aatctcatgg atgatttcct cgatacaaat gacaccttca
     2281 tcacctaatt tctcttcaac gatattgttg tcattcaaga ttatctcttt tggttcttta
     2341 tcttctggcc tttgccacat tattctagtt ctcttttgga ttaaagaacg gatagatgca
     2401 agtgaaggtt gtccgataac aatatatggt gatacaagtt tcaacaatgg gtacacgtcc
     2461 ttggtaagtt tgatgaaaac accagtgttc aactcaacaa gtctcaaaag agataacact
     2521 ttgtaagcct ttgctgggat cttcacagcg atcggatgtg ctgcaaggcg attaagttgg
     2581 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac
     2641 gactcactat agggcgaatt ggagaaacag aataatatat aaacctgtat aatataacct
     2701 tgaagactat atttcttttc gcggccgcct atagggcgaa ttggagctcc ctaggtgctg
     2761 tatatactca cagcataact gtatatacac ccagggtcta ggtgctgtat atactcacag
     2821 cataactgta tatacaccca gggtctaggt gctgtatata ctcacagcat aactgtatat
     2881 acacccaggg tctaggtgct gtatatactc acagcataac tgtatataca cccagggtct
     2941 agagcatgtg ctctgtatgt atataaaact cttgttttct tcttttctct tctttcctta
     3001 tacattagga cctttgcagc ataaattact atacttctat actagtggat cccccgggct
     3061 gcaggaattc atgactgatg aattatttcc aattccagag ccattggtca gaccagttat
     3121 tgctttgcca ccacatttga aagaactgat cgatttgttg ccattgaaca ccccagttca
     3181 tagaagagat ttggaagcta aatacggcag atctaactac gccagaagaa tcagaaagat
     3241 catctctgaa tacggttggg aaatcgaatc tcgtagacaa tctgaaggtg ctaatgatga
     3301 ctggtacatt agaagatctg atggtccagt tagaccacaa agaattagaa gagaagtccc
     3361 aagaagatcc agagaaactg tttacagaag agatgattgg atctgccaaa tctgtagaat
     3421 gaagactgat ccagaaagag gttctttggt tccacaatgc gatcataaga ttccagcaga
     3481 tagaggtggt gattctgatg aagaaaactt gcaaactttg tgcaccagat gcaacttgaa
     3541 aaaaagacaa gcctgtggtg gttgtgcttt ggcttcttgt gctgattgtc catttgctta
     3601 cccagaaaag ttcgatgatg ttctgatctt gcacttggat agagaacact tgaagagaat
     3661 tatgactacc gcttacgcta gaaacgttac tgcttctgct gttgtttctg atttgtctga
     3721 cttgttgcca aagaagaaga gaaaggttga tatcgactac aaggatgacg acgacaagaa
     3781 gctttaacta actaacctcg agacaggccc cttttccttt gtctatatca tgtaattagt
     3841 tatgtcacgc ttacattcac gccctcctcc cacatccgct ctaaccgaaa aggaaggagt
     3901 tagacaacct gaagtctagg tccctattta ttttttttaa tagttatgtt agtattaaga
     3961 acgttattta tatttcaaat ttttcttttt tttctgtaca aacgcgtgta cgcatgtaac
     4021 attatactga aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt aatttgcaag
     4081 gttcgcagtt tacactctca tcgtcgctct catcatcgct tccgttgttg ttttccttag
     4141 tagcgtctgc ttggtaccca gcttcggccg ctctagagaa gcgggtaagc tgccacagca
     4201 attaatgcac aacatttaac ctacattctt ccttatcgga tcctcaaaac ccttaaaaac
     4261 atatgcctca ccctaacata ttttccaatt aaccctcaat atttctctgt cacccggcct
     4321 ctattttcca ttttcttctt tacccgccac gcgttttttt ctttcaaatt tttttcttcc
     4381 ttcttctttt tcttccacgt cctcttgcat aaataaataa accgttttga aaccaaactc
     4441 gcctctctct ctcctttttg aaatattttt gggtttgttt gatcctttcc ttcccaatct
     4501 ctcttgttta atatatattc atttatatca cgctctcttt ttatcttcct ttttttcctc
     4561 tctcttgtat tcttccttcc cctttctact caaaccaaga agaaaaagaa aaggtcaatc
     4621 tttgttaaag aataggatct tctactacat cagcttttag atttttcacg cttactgctt
     4681 ttttcttccc aagatcgaaa atttactgaa ttaacaacta gtggatcccc cgggctgcag
     4741 gaattcatga aagcgttaac ggccaggcaa caagaggtgt ttgatctcat ccgtgatcac
     4801 atcagccaga caggtatgcc gccgacgcgt gcggaaatcg cgcagcgttt ggggttccgt
     4861 tccccaaacg cggctgaaga acatctgaag gcgctggcac gcaaaggcgt tattgaaatt
     4921 gtttccggcg catcacgcgg gattcgtctg ttgcaggaag aggaagaagg gttgccgctg
     4981 gtaggtcgtg tggctgccgg tgaaccactt ctggcgcaac agcatattga aggtcattat
     5041 caggtcgatc cttccttatt caagccgaat gctgatttcc tgctgcgcgt cagcgggatg
     5101 tcgatgaaag acatcggcat tatggatggt gacttgctgg cagtgcataa aactcaggat
     5161 gtacgtaacg gtcaggtcgt tgtcgcacgt attgatgacg aggttaccgt taagcgcctg
     5221 aaaaaacagg gcaataaagt cgaactgttg ccagaaaata gcgagtttaa accaattgtc
     5281 gtagatcttc gtcagcagag cttcaccatt gaagggctgg cggttggggt tattcgcaac
     5341 ggcgactggc tgggttctaa ggatatctct gctggagaca tgagagctgc caacctttgg
     5401 ccaagcccgc tcatgatcaa acgctctaag aagaacagcc tggccttgtc cctgacggcc
     5461 gaccagatgg tcagtgcctt gttggatgct gagcccccca tactctattc cgagtatgat
     5521 cctaccagac ccttcagtga agcttcgatg atgggcttac tgaccaacct ggcagacagg
     5581 gagctggttc acatgatcaa ctgggcgaag agggtgccag gctttgtgga tttgaccctc
     5641 catgatcagg tccaccttct agaatgtgcc tggttagaga tcctgatgat tggtctcgtc
     5701 tggcgctcca tggagcaccc agtgaagcta ctgtttgctc ctaacttgct cttggacagg
     5761 aaccagggaa aatgtgtaga gggcatggtg gagatcttcg acatgctgct ggctacatca
     5821 tctcggttcc gcatgatgaa tctgcaggga gaggagtttg tgtgcctcaa atctattatt
     5881 ttgcttaatt ctggagtgta cacatttctg tccagcaccc tgaagtctct ggaagagaag
     5941 gaccatatcc accgagtcct ggacaagatc acagacactt tgatccacct gatggccaag
     6001 gcaggcctga ccctgcagca gcagcaccag cggctggccc agctcctcct catcctctcc
     6061 cacatcaggc acatgagtaa caaaggcatg gagcatctgt acagcatgaa gtgcaagaac
     6121 gtggtgcccc tctatgacct gctgctggag atgctggacg cccaccgcct acatgcgccc
     6181 actagccgtg gaggggcatc cgtggaggag acggaccaaa gccacttggc cactgcgggc
     6241 tctacttcat cgcattcctt gcaaaagtat tacatcacgg gggaggcaga gggtttccct
     6301 gccacagtcg atatcatgca ggatctgccg ggcaacgata acagcaccgc gggcgaattt
     6361 ccaggtatta ctttgagaat ccaagaaact gatatgttgt ataaaggtga tactttgtat
     6421 ttggattggt tggaagatgg tattgctgaa ttggttttcg atgctccagg ttctgttaat
     6481 aaattggata ctgctgttgc ttctttgggt gaagctattg gtgttttgga acaacaatct
     6541 gatttgattt gggaaacttt gactgttaaa gatgctaaag ttaatttcga ttctggtttg
     6601 gaaaaattcg aagaagctat tccatctgct gatgatttcg atccagttgc tgaaagaaga
     6661 tcctctggtg aatttagagc tgaaagacat tctggtggta ctgatttgtg tttcaagctt
     6721 taaacgcgtt taaagcttat cctcgagaca ggcccctttt cctttgtcta tatcatgtaa
     6781 ttagttatgt cacgcttaca ttcacgccct cctcccacat ccgctctaac cgaaaaggaa
     6841 ggagttagac aacctgaagt ctaggtccct atttattttt tttaatagtt atgttagtat
     6901 taagaacgtt atttatattt caaatttttc ttttttttct gtacaaacgc gtgtacgcat
     6961 gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt
     7021 gcaaggttcg cagtttacac tctcatcgtc gctctcatca tcgcttccgt tgttgttttc
     7081 cttagtagcg tctgcttggt acccagcttc ggccgtattt acagataacg atgtcgttat
     7141 tatatatata tatatatatt gtcacttgta cccagctttt gttcccttta gtgagggtta
     7201 atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7261 acaacagctg taatatatag tagtaacctg aaaaatagtc aatgcccgtg gttcatttgc
     7321 caatagaaca gtcgatgtga tcaatgtaca cgaaagtcaa tataaaatat ccaaatagcc
     7381 aatggtgtca ggacggctaa ggcaaatgca actttgaaat acttattcgg tttgcatctt
     7441 ggcgcgcctc ttcctgcagg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7501 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7561 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7621 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7681 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7741 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7801 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7861 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7921 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7981 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8041 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8101 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     8161 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8221 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8281 aagaagatcc tttgatcttt tgtttaaacc tacggggtct gacgctcagt ggaacgaaaa
     8341 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8401 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8461 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8521 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     8581 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     8641 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     8701 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     8761 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     8821 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     8881 ggttagctcc ttcggtcctc cgatggttgt cagaagtaag ttggccgcag tgttatcact
     8941 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9001 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9061 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9121 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9181 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9241 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9301 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9361 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9421 tccgcgcaca tttccccgaa aagtgccacc t
//
