LOCUS       sequence-302066-        9655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of AsiSI endonuclease in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9655)
  AUTHORS   Gnugge R, Symington LS
  TITLE     Efficient DNA double-strand break formation at single or multiple 
            defined sites in the Saccharomyces cerevisiae genome.
  JOURNAL   Nucleic Acids Res. 2020 Oct 14. pii: 5923424. doi: 
            10.1093/nar/gkaa833.
  PUBMED    33053188
REFERENCE   2  (bases 1 to 9655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Oct 14. pii: 5923424. doi: 10.1093/nar/gkaa833."
COMMENT     Sequence Label: pRG705_lexO-AsiSI_LexA-TF_LEU2MX
FEATURES             Location/Qualifiers
     source          1..9655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2597..2619
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2611..2628
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2612..2628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2635..2654
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2635..2653
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3932..3952
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3959..3982
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      4032..4281
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             join(4951..5202,5203..5556)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     5494..5515
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             5575..6510
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             5623..6513
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     terminator      6977..7226
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(7389..7407)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7428..7444)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7428..7444)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7441..7463)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7452..7468
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(7727..7744)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7898..8486)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7978..7997)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8665..9456,9457..9525))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9288..9307
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9526..9630)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcgaaa gaacgcggca ttagcaacat ttgcgaagcg aacgattttg cgaactgcta
       61 ttaattaact ctggcgcgcc taactttttg tgtggtgccc tcctccttgt caatattaat
      121 gttaaagtgc aattcttttt ccttatcacg ttgagccatt agtatcaatt tgcttacctg
      181 tattccttta catcctcctt tttctccttc ttgataaatg tatgtagatt gcgtatatag
      241 tttcgtctac cctatgaaca tattccattt tgtaatttcg tgtcgcatat gatccgcagg
      301 ctaaccggaa cctgtattat ttagtttatg ctacgttaaa taaagacctt tcgttcacat
      361 aactgaatgt gtaatggcct tgagatttca agcataccaa gttggtggag acggggtcgt
      421 tacaaaagac tctttaagcc aagatttcct tgacagcctt ggcgatagca tcgccaacct
      481 cagtggtaga gttagaacca ccaaggtcac cggttctgac acctgcatcc aagacatttc
      541 taacagcttc ttcaagagcc ctaccttctt caaccaaatc caaggataac ttcaacatca
      601 tagctgcaga taagatggta gcaattgggt taaccttgtt tgctggtaaa tctggggcag
      661 aaccatgaca tggttcgtac aaaccgaatg ccttgttagt gtcaggtagg gaagctagag
      721 atgcagaagg taataaaccc aaagaacctg gaataacaga ggcttcatcg gagataatat
      781 caccaaacat gttgttggta ataacaacac cgtttagctt agttggtgat ttaaccaaaa
      841 tcatagcagc agagtcgatc aattggtgct gaacagttaa ttgtgggaac tcagtcttga
      901 tggtttcttc aacagtcttt ctccacaatc tggaagaggc aagcacgtta gccttgtcaa
      961 gagaccagat tggtaatggt gggttttgtt gcaatgccaa gaaagcagcc attcttgtaa
     1021 ttctttgaac ttcaggaaca ctgtatttct cagagtccca agcaactccg tcaccttcat
     1081 cttcttttct ttcaccaaag tagataccac caaccaattc tctaacgacg acgaaatcag
     1141 taccctttgc atattcaggc ttcaaaggag aaagatctag taaagaatca gaagcaaagt
     1201 tacatggtct caagttggcg tatagaccca attcctttct gatcttcaat agaccttgtt
     1261 ctggtctaac tgcgcccgta ccccattttg gaccaccaac agcacctagt aagacagcat
     1321 cggctttctt agaggcttca agagcttcat ctggtaaagg agtgccagtg gcatcgatgg
     1381 cagcaccccc gatcaagtga tgttggaaat tgaacttaat ttctggacgg acttcagcaa
     1441 tggcattcaa gaccttaata gcttcgtcag taacttcttt accgacgtga tcacccggta
     1501 ggacaacgat attcttagac atggtataat ctgtgtagtg tgggatactt tttacttctt
     1561 caaataggta tcaacaataa aaataaatcg aagcaaatgt aggaatgcgt taaagcagat
     1621 gtacttactt agagtacata gatatattta tataattcaa tatataaaag tatatgaagc
     1681 atctgatgtt gaacctgtca tgactctaaa ctggaatggg tagttatgct tctgaaggtt
     1741 ttccgttaga aaatgagtgc agggatcagg cccggaaccg gttttagcct gaaaggaaaa
     1801 gaaaaaaatg cggtgattac tagcacgtga ctgcgctgaa ttggaatcta ccacaagtac
     1861 agcgaatggc aagagggaag gggaatatat tgtgcctggc ggtagcgtct gctatatcaa
     1921 atgttaaaca taactaaatg cagtagtgaa tagagtaatg tatttctaca tctgtgggta
     1981 gggccaatgg gagcccgatg tctattgtac agatattttc cttttatagt taatcaattc
     2041 aacttggaaa tcattgagtc gatgtctact tggataactg gagccgtggc agcgtttgaa
     2101 atttgacgag tcttcttgtt ttgttcgcgc attttcagtt tattcaaacg ggagatggca
     2161 ccgaatccac tgacttctct gttcaatttg aatggtaata ggaagaaagt acatttcgag
     2221 aaattttcgc ccaacgtcga aatctcatgg atgatttcct cgatacaaat gacaccttca
     2281 tcacctaatt tctcttcaac gatattgttg tcattcaaga ttatctcttt tggttcttta
     2341 tcttctggcc tttgccacat tattctagtt ctcttttgga ttaaagaacg gatagatgca
     2401 agtgaaggtt gtccgataac aatatatggt gatacaagtt tcaacaatgg gtacacgtcc
     2461 ttggtaagtt tgatgaaaac accagtgttc aactcaacaa gtctcaaaag agataacact
     2521 ttgtaagcct ttgctgggat cttcacagcg atcggatgtg ctgcaaggcg attaagttgg
     2581 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac
     2641 gactcactat agggcgaatt ggagaaacag aataatatat aaacctgtat aatataacct
     2701 tgaagactat atttcttttc gcggccgcct atagggcgaa ttggagctcc ctaggtgctg
     2761 tatatactca cagcataact gtatatacac ccagggtcta ggtgctgtat atactcacag
     2821 cataactgta tatacaccca gggtctaggt gctgtatata ctcacagcat aactgtatat
     2881 acacccaggg tctaggtgct gtatatactc acagcataac tgtatataca cccagggtct
     2941 agagcatgtg ctctgtatgt atataaaact cttgttttct tcttttctct tctttcctta
     3001 tacattagga cctttgcagc ataaattact atacttctat actagtggat cccccgggct
     3061 gcaggaattc atgggtgaat ctatcgatca attgaaccca atttctggtg atggtcgttt
     3121 gttgcaatct catggtttga cttctaaggt ttacgtccca atcttgagat acgttttcgc
     3181 tttgagatac aagactggtg atactgttgt tgagttcacc ttggatgata ttagagatgc
     3241 tgctgctgca ttgggtatcg ttgttagaaa cgctgctgat gttatctaca gaatgagatc
     3301 cagaactgtt ttgccagccg aaattttgga attgggtttc tacgttttga cccaagctgg
     3361 tagaggtaga tatagatttg aacaagcctc ctccaccgtt attgatttgc caaacactag
     3421 accaattgaa gccttggatt tgactccaaa tccagttaga agattgctgc cagaacattt
     3481 ggctgatatg gatgaacaag ctattttgat ggttgctggt tactgtaact tgtggcaaca
     3541 ttttactggt ttgaccgttt acagattgag gtcccatgtt agaaaatctg ttccaggtgt
     3601 tggtcaagct gaattggatg aagttaatgt tgctgttgcc gttagagatg atgaagttcc
     3661 agttattatt ccagttgaag ctaaggctgt tgctgatcca gttaacagag ttcaaattgc
     3721 tactcaagtt gctttcgcca atagatactt tgctgaacat gagttcagac ccatgaccat
     3781 taaggttgat agagattccg ttctgaacct gttggagttt aatgctactc caattgcctc
     3841 cgaaattgaa gttattagat ccgccagata caggttgatc ttgtctgata gacaaagaca
     3901 cttgatcgac gaaaccgatc aagttatgtt gccaaagaag aagagaaaag ttgatatcga
     3961 ctacaaggat gacgacgaca agaagcttta actaactaac ctcgagacag gccccttttc
     4021 ctttgtctat atcatgtaat tagttatgtc acgcttacat tcacgccctc ctcccacatc
     4081 cgctctaacc gaaaaggaag gagttagaca acctgaagtc taggtcccta tttatttttt
     4141 ttaatagtta tgttagtatt aagaacgtta tttatatttc aaatttttct tttttttctg
     4201 tacaaacgcg tgtacgcatg taacattata ctgaaaacct tgcttgagaa ggttttggga
     4261 cgctcgaagg ctttaatttg caaggttcgc agtttacact ctcatcgtcg ctctcatcat
     4321 cgcttccgtt gttgttttcc ttagtagcgt ctgcttggta cccagcttcg gccgctctag
     4381 agaagcgggt aagctgccac agcaattaat gcacaacatt taacctacat tcttccttat
     4441 cggatcctca aaacccttaa aaacatatgc ctcaccctaa catattttcc aattaaccct
     4501 caatatttct ctgtcacccg gcctctattt tccattttct tctttacccg ccacgcgttt
     4561 ttttctttca aatttttttc ttccttcttc tttttcttcc acgtcctctt gcataaataa
     4621 ataaaccgtt ttgaaaccaa actcgcctct ctctctcctt tttgaaatat ttttgggttt
     4681 gtttgatcct ttccttccca atctctcttg tttaatatat attcatttat atcacgctct
     4741 ctttttatct tccttttttt cctctctctt gtattcttcc ttcccctttc tactcaaacc
     4801 aagaagaaaa agaaaaggtc aatctttgtt aaagaatagg atcttctact acatcagctt
     4861 ttagattttt cacgcttact gcttttttct tcccaagatc gaaaatttac tgaattaaca
     4921 actagtggat cccccgggct gcaggaattc atgaaagcgt taacggccag gcaacaagag
     4981 gtgtttgatc tcatccgtga tcacatcagc cagacaggta tgccgccgac gcgtgcggaa
     5041 atcgcgcagc gtttggggtt ccgttcccca aacgcggctg aagaacatct gaaggcgctg
     5101 gcacgcaaag gcgttattga aattgtttcc ggcgcatcac gcgggattcg tctgttgcag
     5161 gaagaggaag aagggttgcc gctggtaggt cgtgtggctg ccggtgaacc acttctggcg
     5221 caacagcata ttgaaggtca ttatcaggtc gatccttcct tattcaagcc gaatgctgat
     5281 ttcctgctgc gcgtcagcgg gatgtcgatg aaagacatcg gcattatgga tggtgacttg
     5341 ctggcagtgc ataaaactca ggatgtacgt aacggtcagg tcgttgtcgc acgtattgat
     5401 gacgaggtta ccgttaagcg cctgaaaaaa cagggcaata aagtcgaact gttgccagaa
     5461 aatagcgagt ttaaaccaat tgtcgtagat cttcgtcagc agagcttcac cattgaaggg
     5521 ctggcggttg gggttattcg caacggcgac tggctgggtt ctaaggatat ctctgctgga
     5581 gacatgagag ctgccaacct ttggccaagc ccgctcatga tcaaacgctc taagaagaac
     5641 agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga tgctgagccc
     5701 cccatactct attccgagta tgatcctacc agacccttca gtgaagcttc gatgatgggc
     5761 ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc gaagagggtg
     5821 ccaggctttg tggatttgac cctccatgat caggtccacc ttctagaatg tgcctggtta
     5881 gagatcctga tgattggtct cgtctggcgc tccatggagc acccagtgaa gctactgttt
     5941 gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat ggtggagatc
     6001 ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca gggagaggag
     6061 tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt tctgtccagc
     6121 accctgaagt ctctggaaga gaaggaccat atccaccgag tcctggacaa gatcacagac
     6181 actttgatcc acctgatggc caaggcaggc ctgaccctgc agcagcagca ccagcggctg
     6241 gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg catggagcat
     6301 ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg acctgctgct ggagatgctg
     6361 gacgcccacc gcctacatgc gcccactagc cgtggagggg catccgtgga ggagacggac
     6421 caaagccact tggccactgc gggctctact tcatcgcatt ccttgcaaaa gtattacatc
     6481 acgggggagg cagagggttt ccctgccaca gtcgatatca tgcaggatct gccgggcaac
     6541 gataacagca ccgcgggcga atttccaggt attactttga gaatccaaga aactgatatg
     6601 ttgtataaag gtgatacttt gtatttggat tggttggaag atggtattgc tgaattggtt
     6661 ttcgatgctc caggttctgt taataaattg gatactgctg ttgcttcttt gggtgaagct
     6721 attggtgttt tggaacaaca atctgatttg atttgggaaa ctttgactgt taaagatgct
     6781 aaagttaatt tcgattctgg tttggaaaaa ttcgaagaag ctattccatc tgctgatgat
     6841 ttcgatccag ttgctgaaag aagatcctct ggtgaattta gagctgaaag acattctggt
     6901 ggtactgatt tgtgtttcaa gctttaaacg cgtttaaagc ttatcctcga gacaggcccc
     6961 ttttcctttg tctatatcat gtaattagtt atgtcacgct tacattcacg ccctcctccc
     7021 acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt ccctatttat
     7081 tttttttaat agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt
     7141 ttctgtacaa acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt
     7201 tgggacgctc gaaggcttta atttgcaagg ttcgcagttt acactctcat cgtcgctctc
     7261 atcatcgctt ccgttgttgt tttccttagt agcgtctgct tggtacccag cttcggccgt
     7321 atttacagat aacgatgtcg ttattatata tatatatata tattgtcact tgtacccagc
     7381 ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt
     7441 cctgtgtgaa attgttatcc gctcacaaca gctgtaatat atagtagtaa cctgaaaaat
     7501 agtcaatgcc cgtggttcat ttgccaatag aacagtcgat gtgatcaatg tacacgaaag
     7561 tcaatataaa atatccaaat agccaatggt gtcaggacgg ctaaggcaaa tgcaactttg
     7621 aaatacttat tcggtttgca tcttggcgcg cctcttcctg caggcattaa tgaatcggcc
     7681 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7741 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7801 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7861 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7921 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7981 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8041 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8101 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8161 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8221 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8281 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     8341 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8401 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8461 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttgttta aacctacggg
     8521 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8581 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8641 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8701 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8761 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8821 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8881 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8941 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9001 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9061 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatgg ttgtcagaag
     9121 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9181 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9241 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9301 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9361 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9421 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9481 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9541 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9601 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacct
//
