LOCUS       sequence-302071-        6282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid has been discontinued and a new version is now 
            available. Please see: pGFAP::MyrGFP_v2 (Plasmid #160277) 
            https://www.addgene.org/160277/.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Nam HS, Benezra R
  TITLE     High levels of Id1 expression define B1 type adult neural stem 
            cells.
  JOURNAL   Cell Stem Cell. 2009 Nov 6. 5(5):515-26.
  PUBMED    19896442
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Nov 6. 5(5):515-26."
COMMENT     Sequence Label: pGFAP::MyrGFP
FEATURES             Location/Qualifiers
     source          1..6282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    115..136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(151..156,157..174,175..181)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    189..205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     194..216
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     213..229
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     213..229
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(2611..2613,2614..3321)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     CDS             join(2611..2613,2614..3321)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     primer_bind     complement(2653..2674)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2914..2933)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3261..3282
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3895..3912)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3895..3911)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3904..3926)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4120..4139)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4239..4261
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4299..4317)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4385..4489
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4490..4558,4559..5350)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5521..6109
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6010..6029
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6263..6280
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
       61 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      121 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      181 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
      241 ttcgagctcg gtaccagatc tgagctccca cctccctctc tgtgctggga ctcacagagg
      301 gagacctcag gaggcagtct gtccatcaca tgtccaaatg cagagcatac cctgggctgg
      361 gcgcagtggc gcacaactgt aattccagca ctttgggagg ctgatgtgga aggatcactt
      421 gagcccagaa gttctagacc agcctgggca acatggcaag accctatctc tacaaaaaaa
      481 gttaaaaaat cagccacgtg tggtgacaca cacctgtagt cccagctatt caggaggctg
      541 aggtgagggg atcacttaag gctgggaggt tgaggctgca gtgagtcgtg gttgcgccac
      601 tgcactccag cctgggcaac agtgagaccc tgtctcaaaa gamaaaaaaa aaaaaaaaaa
      661 aaaaaagaac atatcctggt gtggagtagg ggacgctgct ctgacagagg ctcgggggcc
      721 tgagctggct ctgtgagctg gggaggaggc agacagccag gccttgtctg caagcagacc
      781 tggcagcatt gggctggccg ccccccaggg cctcctcttc atgcccagtg aatgactcac
      841 cttggcacag acacaatgtt cggggtgggc acagtgcctg cttcccgccg caccccagcc
      901 cccctcaaat gccttccgag aagcccattg agcagggggc ttgcattgca ccccagcctg
      961 acagcctggc atcttgggat aaaagcagca cagcccccta ggggctgccc ttgctgtgtg
     1021 gcgccaccgg cggtggagaa caaggctcta ttcagcctgt gcccaggaaa ggggatcagg
     1081 ggatgcccag gcatggacag tgggtggcag ggggggagag gagggctgtc tgcttcccag
     1141 aagtccaagg acacaaatgg gtgaggggac tgggcagggt tctgaccctg tgggaccaga
     1201 gtggagggcg tagatggacc tgaagtctcc agggacaaca gggcccaggt ctcaggctcc
     1261 tagttgggcc cagtggctcc agcgtttcca aacccatcca tccccagagg ttcttcccat
     1321 ctctccaggc tgatgtgtgg gaactcgagg aaataaatct ccagtgggag acggaggggt
     1381 ggccagggaa acggggcgct gcaggaataa agacgagcca gcacagccag ctcatgtgta
     1441 acggctttgt ggagctgtca aggcctggtc tctgggagag aggcacaggg aggccagaca
     1501 aggaaggggt gacctggagg gacagatcca ggggctaaag tcctgataag gcaagagagt
     1561 gccggccccc tcttgcccta tcaggacctc cactgccaca tagaggccat gattgaccct
     1621 tagacaaagg gctggtgtcc aatcccagcc cccagcccca gaactccagg gaatgaatgg
     1681 gcagagagca ggaatgtggg acatctgtgt tcaagggaag gactccagga gtctgctggg
     1741 aatgaggcct agtaggaaat gaggtggccc ttgagggtac agaacaggtt cattcttcgc
     1801 caaattccca gcaccttgca ggcacttaca gctgagtgag ataatgcctg ggttatgaaa
     1861 tcaaaaagtt ggaaagcagg tcagaggtca tctggtacag cccttccttc cctttttttt
     1921 tttttttttt gtgagacaag gtctctctct gttgcccagg ctggagtggc gcaaacacag
     1981 ctcactgcag cctcaaccta ctgggctcaa gcaatcctcc agcctcagcc tcccaaagtg
     2041 ctgggattac aagcatgagc caccccactc agccctttcc ttccttttta attgatgcat
     2101 aataattgta agtattcatc atggtccaac caaccctttc ttgacccacc ttcctagaga
     2161 gagggtcctc ttgcttcagc ggtcagggcc ccagacccat ggtctggctc caggtaccac
     2221 ctgcctcatg caggagttgg cgtgcccagg aagctctgcc tctgggcaca gtgacctcag
     2281 tggggtgagg ggagctctcc ccatagctgg gctgcggccc aaccccaccc cctcaggcta
     2341 tgccaggggg tgttgccagg ggcacccggg catcgccagt ctagcccact ccttcataaa
     2401 gccctcgcat cccaggagcg agcagagcca gagcaggttg gagaggagac gcatcacctc
     2461 cgctgctcgc ggggatccac catgggttgc tgtttctcca agaccgcagc gaagggagaa
     2521 gccgccgcgg agaggcctgg ggaggcggct gtggcctcgt cgccttccaa agcgaacgga
     2581 caggagaatg gccacgtgaa ggtaggatcc gtgagcaagg gcgaggagct gttcaccggg
     2641 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     2701 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     2761 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     2821 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     2881 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     2941 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     3001 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     3061 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     3121 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     3181 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     3241 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     3301 ctcggcatgg acgagctgta caataaggat ccgcagatcc cggccagata ccgatgctgc
     3361 cgcagcaaaa gcaggagcag atgccgccgt cgcaggcgaa gatgtcgcag acggaggagg
     3421 cgatgctgcc ggcggaggag gcgaagtaag tagagggctg ggctgggctg tggggggtgt
     3481 ggggtgcggg actgggcagt ctgggagtcc ctctcaccac ttttcttacc tttctaggat
     3541 gctgccgtcg ccgccgctca tacaccataa ggtgtaaaaa atactagatg cacagaatag
     3601 caagtccatc aaaactcctg cgtgagaatt ttaccagact tcaagagcat ctcgccacat
     3661 cttgaaaaat gccaccgtcc gatgaaaaac aggagcctgc taaggaacaa tgccacctgt
     3721 caataaatgt tgaaaactca tcccattcct gcctcttggt ccttgggctt ggggaggggt
     3781 gcgcggatgt ggttagggaa catgactggt caaatgggaa gggcttcaaa agaattccca
     3841 atattgacta ccaagccacc tgtacagatc tgcctgcagg catgcaagct tggcactggc
     3901 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     3961 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     4021 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     4081 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     4141 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     4201 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     4261 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     4321 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     4381 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4441 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     4501 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     4561 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     4621 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     4681 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     4741 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     4801 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     4861 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     4921 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     4981 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5041 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5101 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     5161 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     5221 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     5281 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     5341 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     5401 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     5461 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5521 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5581 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5641 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     5701 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5761 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     5821 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5881 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5941 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6001 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6061 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6121 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     6181 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     6241 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag ga
//
