LOCUS       sequence-302074-        9171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of HO endonuclease in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Gnugge R, Symington LS
  TITLE     Efficient DNA double-strand break formation at single or multiple 
            defined sites in the Saccharomyces cerevisiae genome.
  JOURNAL   Nucleic Acids Res. 2020 Oct 14. pii: 5923424. doi: 
            10.1093/nar/gkaa833.
  PUBMED    33053188
REFERENCE   2  (bases 1 to 9171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Oct 14. pii: 5923424. doi: 10.1093/nar/gkaa833."
COMMENT     Sequence Label: pRG710_lexO-HO_LexA-TF_HIS3MX
FEATURES             Location/Qualifiers
     source          1..9171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             273..923
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /translation="MRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHANQK
                     GEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQA
                     MGNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLYS
                     FSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
     primer_bind     1262..1284
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1276..1293
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1277..1293
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1300..1319
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1300..1318
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2885..2896
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             3500..3523
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      3573..3822
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             join(4492..4743,4744..5097)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     5035..5056
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             5116..6051
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             5164..6054
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     terminator      6518..6767
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(6930..6948)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6969..6985)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6969..6985)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6982..7004)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6993..7009
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(7243..7260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7414..8002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7494..7513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8181..8972,8973..9041))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8804..8823
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9042..9146)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcgaaa gaacgcggca ttagcaacat ttgcgaagcg aacgattttg cgaactgcta
       61 ttaattaact ctggcgcgcc tcctctagta cactctatat ttttttatgc ctcggtaatg
      121 attttcattt ttttttttcc acctagcgga tgactctttt tttttcttag cgattggcat
      181 tatcacataa tgaattatac attatataaa gtaatgtgat ttcttcgaag aatatactaa
      241 aaaatgagca ggcaagataa acgaaggcac atatgaggag ggcttttgta gaaagaaata
      301 cgaacgaaac gaaaatcagc gttgccatcg ctttggacaa agctccctta cctgaagagt
      361 cgaattttat tgatgaactt ataacttcca agcatgcaaa ccaaaaggga gaacaagtaa
      421 tccaagtaga cacgggaatt ggattcttgg atcacatgta tcatgcactg gctaaacatg
      481 caggctggag cttacgactt tactcaagag gtgatttaat catcgatgat catcacactg
      541 cagaagatac tgctattgca cttggtattg cattcaagca ggctatgggt aactttgccg
      601 gtgttaaaag atttggacat gcttattgtc cacttgacga agctctttcc agaagcgtag
      661 ttgacttgtc gggacggccc tatgctgtta tcgatttggg attaaagcgt gaaaaggttg
      721 gggaattgtc ctgtgaaatg atccctcact tactatattc cttttcggta gctgctggaa
      781 ttactttgca tgttacctgc ttatatggta gtaatgacca tcatcgtgct gaaagcgctt
      841 ttaaatctct ggctgttgcc atgcgcgcgg ctacttctct tactggaagt tctgaagtcc
      901 caagcacgaa gggagtgttg taaagatgaa ttggattatg tcaggaaaag aacgacaatt
      961 ttgcatccaa attgtctaaa ttttagagtt gcttgaaaac aatagaacct tacttgcttt
     1021 ataattacgt taattagaag cgttatctcg tgaaggaata tagtacgtag ccgtataaat
     1081 tgaattgaat gttcagctta tagaatagag acactttgct gttcaatgcg tcgtcactta
     1141 ccatactcac tttattatac gactttaagt ataaactccg cggttatggt aaaattaatg
     1201 atgcacaaac gtccgatcgg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     1261 tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc actatagggc
     1321 gaattggaga aacagaataa tatataaacc tgtataatat aaccttgaag actatatttc
     1381 ttttcgcggc cgcctatagg gcgaattgga gctccctagg tgctgtatat actcacagca
     1441 taactgtata tacacccagg gtctaggtgc tgtatatact cacagcataa ctgtatatac
     1501 acccagggtc taggtgctgt atatactcac agcataactg tatatacacc cagggtctag
     1561 gtgctgtata tactcacagc ataactgtat atacacccag ggtctagagc atgtgctctg
     1621 tatgtatata aaactcttgt tttcttcttt tctcttcttt ccttatacat taggaccttt
     1681 gcagcataaa ttactatact tctatactag tggatccccc gggctgcagg aattcatgct
     1741 ttctgaaaac acgactattc tgatggctaa cggtgaaatt aaagacatcg caaacgtcac
     1801 ggctaactct tacgttatgt gcgcagatgg ctccgctgcc cgcgtcataa atgtcacaca
     1861 gggctatcag aaaatctata atatacagca aaaaaccaaa cacagagctt ttgaaggtga
     1921 acctggtagg ttagatccca ggcgtagaac agtttatcag cgtcttgcat tacaatgtac
     1981 tgcaggtcat aaattgtcag tcagggtccc taccaaacca ctgttggaaa aaagtggtag
     2041 aaatgccacc aaatataaag tgagatggag aaatctgcag caatgtcaga cgcttgatgg
     2101 taggataata ataattccaa aaaaccatca taagacattc ccaatgacag ttgaaggtga
     2161 gtttgccgca aaacgcttca tagaagaaat ggagcgctct aaaggagaat atttcaactt
     2221 tgacattgaa gttagagatt tggattatct tgatgctcaa ttgagaattt ctagctgcat
     2281 aagatttggt ccagtactca caggaaatgg tgttttatct aaatttctca ctggacgtag
     2341 tgaccttgta actcctgctg taaaaagtat ggcttggatg cttggtctgt ggttaggtga
     2401 cggtacaaca aaagagccag aaatctcagt agatagcttg gatcctaagc taatggagag
     2461 tttaagagaa aatgcgaaaa tctggggtct ctaccttacg gtttgtgacg atcacgttcc
     2521 gctacgtgcc aaacatgtaa ggcttcatta tggagatggt ccagatgaaa acaggaagac
     2581 aaggaatttg aggaaaaata atccattctg gaaagctgtc acaattttaa agtttaaaag
     2641 ggatcttgat ggagagaagc aaatccctga atttatgtac ggcgagcata tagaagttcg
     2701 tgaagcattc ttagccggct tgatcgactc agatgggtac gttgtgaaaa agggcgaagg
     2761 ccctgaatct tataaaatag caattcaaac tgtttattca tccattatgg acggaattgt
     2821 ccatatttca agatctcttg gtatgtcagc tactgtgacg accaggtcag ctagggagga
     2881 aatcattgaa ggaagaaaag tccaatgtca atttacatac gactgtaatg ttgctggggg
     2941 aacaacttta cagaatgttt tgtcatattg tcgaagtggt cacaaaacaa gagaagttcc
     3001 gccaattata aaaagggaac ccgtatattt cagcttcacg gatgatttcc agggtgagag
     3061 tactgtatat gggcttacga tagaaggcca taaaaatttc ttgcttggca acaaaataga
     3121 agtgaaatca tgtcgaggct gctgtgtggg agaacagcat aaaatatcac aaaaaaagaa
     3181 tctaaaacac tgtgttgctt gtcccagaaa gggaatcaag tatttttata aagattggag
     3241 tggtaaaaat cgagtatgtg ctagatgcta tggaagatac aaattcagcg gtcatcactg
     3301 tataaattgc aagtatgtac cagaagcacg tgaagtgaaa aaggcaaaag acaaaggcga
     3361 aaaattgggc attacgcccg aaggtttgcc agttaaagga ccagagtgta taaaatgtgg
     3421 cggaatctta cagtttgatg ctgtccgcgg gcctcataag agttgtggta acaacgcagg
     3481 tgcgcgcatc tgcgatatcg actacaagga tgacgacgac aagaagcttt aactaactaa
     3541 cctcgagaca ggcccctttt cctttgtcta tatcatgtaa ttagttatgt cacgcttaca
     3601 ttcacgccct cctcccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt
     3661 ctaggtccct atttattttt tttaatagtt atgttagtat taagaacgtt atttatattt
     3721 caaatttttc ttttttttct gtacaaacgc gtgtacgcat gtaacattat actgaaaacc
     3781 ttgcttgaga aggttttggg acgctcgaag gctttaattt gcaaggttcg cagtttacac
     3841 tctcatcgtc gctctcatca tcgcttccgt tgttgttttc cttagtagcg tctgcttggt
     3901 acccagcttc ggccgctcta gagaagcggg taagctgcca cagcaattaa tgcacaacat
     3961 ttaacctaca ttcttcctta tcggatcctc aaaaccctta aaaacatatg cctcacccta
     4021 acatattttc caattaaccc tcaatatttc tctgtcaccc ggcctctatt ttccattttc
     4081 ttctttaccc gccacgcgtt tttttctttc aaattttttt cttccttctt ctttttcttc
     4141 cacgtcctct tgcataaata aataaaccgt tttgaaacca aactcgcctc tctctctcct
     4201 ttttgaaata tttttgggtt tgtttgatcc tttccttccc aatctctctt gtttaatata
     4261 tattcattta tatcacgctc tctttttatc ttcctttttt tcctctctct tgtattcttc
     4321 cttccccttt ctactcaaac caagaagaaa aagaaaaggt caatctttgt taaagaatag
     4381 gatcttctac tacatcagct tttagatttt tcacgcttac tgcttttttc ttcccaagat
     4441 cgaaaattta ctgaattaac aactagtgga tcccccgggc tgcaggaatt catgaaagcg
     4501 ttaacggcca ggcaacaaga ggtgtttgat ctcatccgtg atcacatcag ccagacaggt
     4561 atgccgccga cgcgtgcgga aatcgcgcag cgtttggggt tccgttcccc aaacgcggct
     4621 gaagaacatc tgaaggcgct ggcacgcaaa ggcgttattg aaattgtttc cggcgcatca
     4681 cgcgggattc gtctgttgca ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct
     4741 gccggtgaac cacttctggc gcaacagcat attgaaggtc attatcaggt cgatccttcc
     4801 ttattcaagc cgaatgctga tttcctgctg cgcgtcagcg ggatgtcgat gaaagacatc
     4861 ggcattatgg atggtgactt gctggcagtg cataaaactc aggatgtacg taacggtcag
     4921 gtcgttgtcg cacgtattga tgacgaggtt accgttaagc gcctgaaaaa acagggcaat
     4981 aaagtcgaac tgttgccaga aaatagcgag tttaaaccaa ttgtcgtaga tcttcgtcag
     5041 cagagcttca ccattgaagg gctggcggtt ggggttattc gcaacggcga ctggctgggt
     5101 tctaaggata tctctgctgg agacatgaga gctgccaacc tttggccaag cccgctcatg
     5161 atcaaacgct ctaagaagaa cagcctggcc ttgtccctga cggccgacca gatggtcagt
     5221 gccttgttgg atgctgagcc ccccatactc tattccgagt atgatcctac cagacccttc
     5281 agtgaagctt cgatgatggg cttactgacc aacctggcag acagggagct ggttcacatg
     5341 atcaactggg cgaagagggt gccaggcttt gtggatttga ccctccatga tcaggtccac
     5401 cttctagaat gtgcctggtt agagatcctg atgattggtc tcgtctggcg ctccatggag
     5461 cacccagtga agctactgtt tgctcctaac ttgctcttgg acaggaacca gggaaaatgt
     5521 gtagagggca tggtggagat cttcgacatg ctgctggcta catcatctcg gttccgcatg
     5581 atgaatctgc agggagagga gtttgtgtgc ctcaaatcta ttattttgct taattctgga
     5641 gtgtacacat ttctgtccag caccctgaag tctctggaag agaaggacca tatccaccga
     5701 gtcctggaca agatcacaga cactttgatc cacctgatgg ccaaggcagg cctgaccctg
     5761 cagcagcagc accagcggct ggcccagctc ctcctcatcc tctcccacat caggcacatg
     5821 agtaacaaag gcatggagca tctgtacagc atgaagtgca agaacgtggt gcccctctat
     5881 gacctgctgc tggagatgct ggacgcccac cgcctacatg cgcccactag ccgtggaggg
     5941 gcatccgtgg aggagacgga ccaaagccac ttggccactg cgggctctac ttcatcgcat
     6001 tccttgcaaa agtattacat cacgggggag gcagagggtt tccctgccac agtcgatatc
     6061 atgcaggatc tgccgggcaa cgataacagc accgcgggcg aatttccagg tattactttg
     6121 agaatccaag aaactgatat gttgtataaa ggtgatactt tgtatttgga ttggttggaa
     6181 gatggtattg ctgaattggt tttcgatgct ccaggttctg ttaataaatt ggatactgct
     6241 gttgcttctt tgggtgaagc tattggtgtt ttggaacaac aatctgattt gatttgggaa
     6301 actttgactg ttaaagatgc taaagttaat ttcgattctg gtttggaaaa attcgaagaa
     6361 gctattccat ctgctgatga tttcgatcca gttgctgaaa gaagatcctc tggtgaattt
     6421 agagctgaaa gacattctgg tggtactgat ttgtgtttca agctttaaac gcgtttaaag
     6481 cttatcctcg agacaggccc cttttccttt gtctatatca tgtaattagt tatgtcacgc
     6541 ttacattcac gccctcctcc cacatccgct ctaaccgaaa aggaaggagt tagacaacct
     6601 gaagtctagg tccctattta ttttttttaa tagttatgtt agtattaaga acgttattta
     6661 tatttcaaat ttttcttttt tttctgtaca aacgcgtgta cgcatgtaac attatactga
     6721 aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt aatttgcaag gttcgcagtt
     6781 tacactctca tcgtcgctct catcatcgct tccgttgttg ttttccttag tagcgtctgc
     6841 ttggtaccca gcttcggccg tatttacaga taacgatgtc gttattatat atatatatat
     6901 atattgtcac ttgtacccag cttttgttcc ctttagtgag ggttaatttc gagcttggcg
     6961 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaac agctgacacc
     7021 gattatttaa agctgcagca tacgatatat atacatgtgt atatatgtat acctatgaat
     7081 gtcagtaagt atgtatacga acagtatgat actgaagatg acaaggtaat gcatcattct
     7141 atacgtgtca ttctgaacga ggcgcgcctc tccctgcagg cattaatgaa tcggccaacg
     7201 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     7261 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     7321 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     7381 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7441 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7501 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7561 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7621 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7681 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7741 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7801 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     7861 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7921 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7981 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tgtttaaacc tacggggtct
     8041 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8101 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8161 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8221 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8281 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8341 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8401 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8461 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8521 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8581 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatggttgt cagaagtaag
     8641 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8701 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8761 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8821 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8881 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8941 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9001 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9061 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9121 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc t
//
