LOCUS       sequence-302076-        8799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of AscI endonuclease in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Gnugge R, Symington LS
  TITLE     Efficient DNA double-strand break formation at single or multiple 
            defined sites in the Saccharomyces cerevisiae genome.
  JOURNAL   Nucleic Acids Res. 2020 Oct 14. pii: 5923424. doi: 
            10.1093/nar/gkaa833.
  PUBMED    33053188
REFERENCE   2  (bases 1 to 8799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Oct 14. pii: 5923424. doi: 10.1093/nar/gkaa833."
COMMENT     Sequence Label: pRG711_lexO-AscI_LexA-TF_HIS3MX
FEATURES             Location/Qualifiers
     source          1..8799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             273..923
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /translation="MRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHANQK
                     GEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQA
                     MGNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLYS
                     FSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
     primer_bind     1262..1284
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1276..1293
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1277..1293
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1300..1319
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1300..1318
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3101..3121
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3128..3151
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      3201..3450
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             join(4120..4371,4372..4725)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     4663..4684
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             4744..5679
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4792..5682
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     terminator      6146..6395
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(6558..6576)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6597..6613)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6597..6613)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6610..6632)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6621..6637
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(6871..6888)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7042..7630)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7122..7141)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7809..8600,8601..8669))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8432..8451
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8670..8774)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcgaaa gaacgcggca ttagcaacat ttgcgaagcg aacgattttg cgaactgcta
       61 ttaattaact ctggcgcgcc tcctctagta cactctatat ttttttatgc ctcggtaatg
      121 attttcattt ttttttttcc acctagcgga tgactctttt tttttcttag cgattggcat
      181 tatcacataa tgaattatac attatataaa gtaatgtgat ttcttcgaag aatatactaa
      241 aaaatgagca ggcaagataa acgaaggcac atatgaggag ggcttttgta gaaagaaata
      301 cgaacgaaac gaaaatcagc gttgccatcg ctttggacaa agctccctta cctgaagagt
      361 cgaattttat tgatgaactt ataacttcca agcatgcaaa ccaaaaggga gaacaagtaa
      421 tccaagtaga cacgggaatt ggattcttgg atcacatgta tcatgcactg gctaaacatg
      481 caggctggag cttacgactt tactcaagag gtgatttaat catcgatgat catcacactg
      541 cagaagatac tgctattgca cttggtattg cattcaagca ggctatgggt aactttgccg
      601 gtgttaaaag atttggacat gcttattgtc cacttgacga agctctttcc agaagcgtag
      661 ttgacttgtc gggacggccc tatgctgtta tcgatttggg attaaagcgt gaaaaggttg
      721 gggaattgtc ctgtgaaatg atccctcact tactatattc cttttcggta gctgctggaa
      781 ttactttgca tgttacctgc ttatatggta gtaatgacca tcatcgtgct gaaagcgctt
      841 ttaaatctct ggctgttgcc atgcgcgcgg ctacttctct tactggaagt tctgaagtcc
      901 caagcacgaa gggagtgttg taaagatgaa ttggattatg tcaggaaaag aacgacaatt
      961 ttgcatccaa attgtctaaa ttttagagtt gcttgaaaac aatagaacct tacttgcttt
     1021 ataattacgt taattagaag cgttatctcg tgaaggaata tagtacgtag ccgtataaat
     1081 tgaattgaat gttcagctta tagaatagag acactttgct gttcaatgcg tcgtcactta
     1141 ccatactcac tttattatac gactttaagt ataaactccg cggttatggt aaaattaatg
     1201 atgcacaaac gtccgatcgg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     1261 tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc actatagggc
     1321 gaattggaga aacagaataa tatataaacc tgtataatat aaccttgaag actatatttc
     1381 ttttcgcggc cgcctatagg gcgaattgga gctccctagg tgctgtatat actcacagca
     1441 taactgtata tacacccagg gtctaggtgc tgtatatact cacagcataa ctgtatatac
     1501 acccagggtc taggtgctgt atatactcac agcataactg tatatacacc cagggtctag
     1561 gtgctgtata tactcacagc ataactgtat atacacccag ggtctagagc atgtgctctg
     1621 tatgtatata aaactcttgt tttcttcttt tctcttcttt ccttatacat taggaccttt
     1681 gcagcataaa ttactatact tctatactag tggatccccc gggctgcagg aattcatgat
     1741 tgaatttcca gagtacagag attcttctgc tgctccaaaa atctccgatt tggaaagatt
     1801 gatggacagg tctttgacca actctcaata tgttgatggt gctaacgctg ctagattatt
     1861 gggtactttc atcagatcct tcagatccgt tattggttct gctgaagaat ctgctactag
     1921 agctaatttg gttgaagctc atgatgaagc taagttgttc ggtttgatgt tgtctgctgg
     1981 tttcgatttg atctgtaacg ctgaatacgt tcacggtaga ttggttaaca acaagtggat
     2041 ctattgtcac agaggtggtg aaccagcagt tgcttattac tctttcttga aacaatgtcc
     2101 aagatgctgc ttggatagag gtttggaagg tagattgtct ggtgctcaac ataagccaac
     2161 ttctcatcat attggtgaaa ttaccaccgt tgctattgcc ttgttgttgc aattagttgc
     2221 tgctgctaac gaaaacccat tcgaaattgc tactatcacc aagcaatccc atgatgttga
     2281 tgctattggt ttcagagatg acttgttggt cttgttcgaa atcaaggctt ctccaatggt
     2341 ttctttccca ttggttactg aattggaaga acctatgttg caagaaggtc cagacggtcc
     2401 agttgagtat agacaacatt ctttggttga cttgacgttg cagggtagag aatttgctgt
     2461 tgctattcca catgctgaaa cagctattcc attgggtgaa agagaaggcg aatcttggcc
     2521 atacgaacca ttgattgatt acttttctgt tccagctaat gccgcttctt acttgcaagc
     2581 ctggattgaa ttatatgctg cttacagaac tcccaagact caaagagctg gtagaactgc
     2641 tgctttggct tatttggtta atggttgggg tgacgaaatc gattctaaca aaacaaaacc
     2701 aggtttgggt agaaccgatg atgttaagaa aggtacttac cagttgttga agttcggttc
     2761 ctattacaga gatgatgctg catcagttcc agttagaggt gctttggttg ctaacttaga
     2821 tcctttgttt ttgaggccag gttacatcga tggtttgtct gatgttagat ggggtcatgg
     2881 tagagatttc actttggaag aaggtgagta cagaattgct gaaggttctt tgagacactt
     2941 gtacgatgct attttggctt tcaatgaccc tttgttgaat gacccattgc tgcaagaaat
     3001 ctttgatttg ggtgctgtcg aaagaaagtt ggctaatggt gatttggaag ccttgttgga
     3061 taagtggatt gctagaccag aaatagtttt ggacccatct ccaaagaaga agagaaaagt
     3121 tgatatcgac tacaaggatg acgacgacaa gaagctttaa ctaactaacc tcgagacagg
     3181 ccccttttcc tttgtctata tcatgtaatt agttatgtca cgcttacatt cacgccctcc
     3241 tcccacatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat
     3301 ttattttttt taatagttat gttagtatta agaacgttat ttatatttca aatttttctt
     3361 ttttttctgt acaaacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag
     3421 gttttgggac gctcgaaggc tttaatttgc aaggttcgca gtttacactc tcatcgtcgc
     3481 tctcatcatc gcttccgttg ttgttttcct tagtagcgtc tgcttggtac ccagcttcgg
     3541 ccgctctaga gaagcgggta agctgccaca gcaattaatg cacaacattt aacctacatt
     3601 cttccttatc ggatcctcaa aacccttaaa aacatatgcc tcaccctaac atattttcca
     3661 attaaccctc aatatttctc tgtcacccgg cctctatttt ccattttctt ctttacccgc
     3721 cacgcgtttt tttctttcaa atttttttct tccttcttct ttttcttcca cgtcctcttg
     3781 cataaataaa taaaccgttt tgaaaccaaa ctcgcctctc tctctccttt ttgaaatatt
     3841 tttgggtttg tttgatcctt tccttcccaa tctctcttgt ttaatatata ttcatttata
     3901 tcacgctctc tttttatctt cctttttttc ctctctcttg tattcttcct tcccctttct
     3961 actcaaacca agaagaaaaa gaaaaggtca atctttgtta aagaatagga tcttctacta
     4021 catcagcttt tagatttttc acgcttactg cttttttctt cccaagatcg aaaatttact
     4081 gaattaacaa ctagtggatc ccccgggctg caggaattca tgaaagcgtt aacggccagg
     4141 caacaagagg tgtttgatct catccgtgat cacatcagcc agacaggtat gccgccgacg
     4201 cgtgcggaaa tcgcgcagcg tttggggttc cgttccccaa acgcggctga agaacatctg
     4261 aaggcgctgg cacgcaaagg cgttattgaa attgtttccg gcgcatcacg cgggattcgt
     4321 ctgttgcagg aagaggaaga agggttgccg ctggtaggtc gtgtggctgc cggtgaacca
     4381 cttctggcgc aacagcatat tgaaggtcat tatcaggtcg atccttcctt attcaagccg
     4441 aatgctgatt tcctgctgcg cgtcagcggg atgtcgatga aagacatcgg cattatggat
     4501 ggtgacttgc tggcagtgca taaaactcag gatgtacgta acggtcaggt cgttgtcgca
     4561 cgtattgatg acgaggttac cgttaagcgc ctgaaaaaac agggcaataa agtcgaactg
     4621 ttgccagaaa atagcgagtt taaaccaatt gtcgtagatc ttcgtcagca gagcttcacc
     4681 attgaagggc tggcggttgg ggttattcgc aacggcgact ggctgggttc taaggatatc
     4741 tctgctggag acatgagagc tgccaacctt tggccaagcc cgctcatgat caaacgctct
     4801 aagaagaaca gcctggcctt gtccctgacg gccgaccaga tggtcagtgc cttgttggat
     4861 gctgagcccc ccatactcta ttccgagtat gatcctacca gacccttcag tgaagcttcg
     4921 atgatgggct tactgaccaa cctggcagac agggagctgg ttcacatgat caactgggcg
     4981 aagagggtgc caggctttgt ggatttgacc ctccatgatc aggtccacct tctagaatgt
     5041 gcctggttag agatcctgat gattggtctc gtctggcgct ccatggagca cccagtgaag
     5101 ctactgtttg ctcctaactt gctcttggac aggaaccagg gaaaatgtgt agagggcatg
     5161 gtggagatct tcgacatgct gctggctaca tcatctcggt tccgcatgat gaatctgcag
     5221 ggagaggagt ttgtgtgcct caaatctatt attttgctta attctggagt gtacacattt
     5281 ctgtccagca ccctgaagtc tctggaagag aaggaccata tccaccgagt cctggacaag
     5341 atcacagaca ctttgatcca cctgatggcc aaggcaggcc tgaccctgca gcagcagcac
     5401 cagcggctgg cccagctcct cctcatcctc tcccacatca ggcacatgag taacaaaggc
     5461 atggagcatc tgtacagcat gaagtgcaag aacgtggtgc ccctctatga cctgctgctg
     5521 gagatgctgg acgcccaccg cctacatgcg cccactagcc gtggaggggc atccgtggag
     5581 gagacggacc aaagccactt ggccactgcg ggctctactt catcgcattc cttgcaaaag
     5641 tattacatca cgggggaggc agagggtttc cctgccacag tcgatatcat gcaggatctg
     5701 ccgggcaacg ataacagcac cgcgggcgaa tttccaggta ttactttgag aatccaagaa
     5761 actgatatgt tgtataaagg tgatactttg tatttggatt ggttggaaga tggtattgct
     5821 gaattggttt tcgatgctcc aggttctgtt aataaattgg atactgctgt tgcttctttg
     5881 ggtgaagcta ttggtgtttt ggaacaacaa tctgatttga tttgggaaac tttgactgtt
     5941 aaagatgcta aagttaattt cgattctggt ttggaaaaat tcgaagaagc tattccatct
     6001 gctgatgatt tcgatccagt tgctgaaaga agatcctctg gtgaatttag agctgaaaga
     6061 cattctggtg gtactgattt gtgtttcaag ctttaaacgc gtttaaagct tatcctcgag
     6121 acaggcccct tttcctttgt ctatatcatg taattagtta tgtcacgctt acattcacgc
     6181 cctcctccca catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc
     6241 cctatttatt ttttttaata gttatgttag tattaagaac gttatttata tttcaaattt
     6301 ttcttttttt tctgtacaaa cgcgtgtacg catgtaacat tatactgaaa accttgcttg
     6361 agaaggtttt gggacgctcg aaggctttaa tttgcaaggt tcgcagttta cactctcatc
     6421 gtcgctctca tcatcgcttc cgttgttgtt ttccttagta gcgtctgctt ggtacccagc
     6481 ttcggccgta tttacagata acgatgtcgt tattatatat atatatatat attgtcactt
     6541 gtacccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca
     6601 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaacag ctgacaccga ttatttaaag
     6661 ctgcagcata cgatatatat acatgtgtat atatgtatac ctatgaatgt cagtaagtat
     6721 gtatacgaac agtatgatac tgaagatgac aaggtaatgc atcattctat acgtgtcatt
     6781 ctgaacgagg cgcgcctctc cctgcaggca ttaatgaatc ggccaacgcg cggggagagg
     6841 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6901 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6961 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7021 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7081 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7141 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7201 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7261 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7321 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7381 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7441 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7501 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7561 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7621 aggatctcaa gaagatcctt tgatcttttg tttaaaccta cggggtctga cgctcagtgg
     7681 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7741 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7801 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7861 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7921 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7981 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8041 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8101 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8161 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8221 aaaaaagcgg ttagctcctt cggtcctccg atggttgtca gaagtaagtt ggccgcagtg
     8281 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8341 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8401 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8461 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8521 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8581 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8641 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8701 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8761 ataggggttc cgcgcacatt tccccgaaaa gtgccacct
//
