LOCUS       sequence-302078-        6512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse astrocyte expression of Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Zhuo L, Theis M, Alvarez-Maya I, Brenner M, Willecke K, Messing A
  TITLE     hGFAP-cre transgenic mice for manipulation of glial and neuronal 
            function in vivo.
  JOURNAL   Genesis. 2001 Oct;31(2):85-94.
  PUBMED    11668683
REFERENCE   2  (bases 1 to 6512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis."; 
            date: "2001-10"; volume: "31(2)"; pages: "85-94"
COMMENT     Sequence Label: hGFAP-Cre
FEATURES             Location/Qualifiers
     source          1..6512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    115..136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(151..156,157..174,175..181)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    189..205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     194..216
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     213..229
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     213..229
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2486..2506
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2507..3535
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     primer_bind     complement(4125..4142)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4125..4141)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4134..4156)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4350..4369)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4469..4491
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4529..4547)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4615..4719
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4720..4788,4789..5580)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5751..6339
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6240..6259
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6493..6510
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
       61 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      121 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      181 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
      241 ttcgagctcg gtaccagatc tgagctccca cctccctctc tgtgctggga ctcacagagg
      301 gagacctcag gaggcagtct gtccatcaca tgtccaaatg cagagcatac cctgggctgg
      361 gcgcagtggc gcacaactgt aattccagca ctttgggagg ctgatgtgga aggatcactt
      421 gagcccagaa gttctagacc agcctgggca acatggcaag accctatctc tacaaaaaaa
      481 gttaaaaaat cagccacgtg tggtgacaca cacctgtagt cccagctatt caggaggctg
      541 aggtgagggg atcacttaag gctgggaggt tgaggctgca gtgagtcgtg gttgcgccac
      601 tgcactccag cctgggcaac agtgagaccc tgtctcaaaa gacaaaaaaa aaaaaaaaaa
      661 aaaaangaac atatcctggt gtggagtagg ggacgctgct ctgacagagg ctcgggggcc
      721 tgagctggct ctgtgagctg gggaggaggc agacagccag gccttgtctg caagcagacc
      781 tggcagcatt gggctggccg ccccccaggg cctcctcttc atgcccagtg aatgactcac
      841 cttggcacag acacaatgtt cggggtgggc acagtgcctg cttcccgccg caccccagcc
      901 cccctcaaat gccttccgag aagcccattg agcagggggc ttgcattgca ccccagcctg
      961 acagcctggc atcttgggat aaaagcagca cagcccccta ggggctgccc ttgctgtgtg
     1021 gcgccaccgg cggtggagaa caaggctcta ttcagcctgt gcccaggaaa ggggatcagg
     1081 ggatgcccag gcatggacag tgggtggcag ggggggagag gagggctgtc tgcttcccag
     1141 aagtccaagg acacaaatgg gtgaggggac tgggcagggt tctgaccctg tgggaccaga
     1201 gtggagggcg tagatggacc tgaagtctcc agggacaaca gggcccaggt ctcaggctcc
     1261 tagttgggcc cagtggctcc agcgtttcca aacccatcca tccccagagg ttcttcccat
     1321 ctctccaggc tgatgtgtgg gaactcgagg aaataaatct ccagtgggag acggaggggt
     1381 ggccagggaa acggggcgct gcaggaataa agacgagcca gcacagccag ctcatgtgta
     1441 acggctttgt ggagctgtca aggcctggtc tctgggagag aggcacaggg aggccagaca
     1501 aggaaggggt gacctggagg gacagatcca ggggctaaag tcctgataag gcaagagagt
     1561 gccggccccc tcttgcccta tcaggacctc cactgccaca tagaggccat gattgaccct
     1621 tagacaaagg gctggtgtcc aatcccagcc cccagcccca gaactccagg gaatgaatgg
     1681 gcagagagca ggaatgtggg acatctgtgt tcaagggaag gactccagga gtctgctggg
     1741 aatgaggcct agtaggaaat gaggtggccc ttgagggtac agaacaggtt cattcttcgc
     1801 caaattccca gcaccttgca ggcacttaca gctgagtgag ataatgcctg ggttatgaaa
     1861 tcaaaaagtt ggaaagcagg tcagaggtca tctggtacag cccttccttc cctttttttt
     1921 tttttttttt gtgagacaag gtctctctct gttgcccagg ctggagtggc gcaaacacag
     1981 ctcactgcag cctcaaccta ctgggctcaa gcaatcctcc agcctcagcc tcccaaagtg
     2041 ctgggattac aagcatgagc caccccactc agccctttcc ttccttttta attgatgcat
     2101 aataattgta agtattcatc atggtccaac caaccctttc ttgacccacc ttcctagaga
     2161 gagggtcctc ttgcttcagc ggtcagggcc ccagacccat ggtctggctc caggtaccac
     2221 ctgcctcatg caggagttgg cgtgcccagg aagctctgcc tctgggcaca gtgacctcag
     2281 tggggtgagg ggagctctcc ccatagctgg gctgcggccc aaccccaccc cctcaggcta
     2341 tgccaggggg tgttgccagg ggcacccggg catcgccagt ctagcccact ccttcataaa
     2401 gccctcgcat cccaggagcg agcagagcca gagcaggttg gagaggagac gcatcacctc
     2461 cgctgctcgc ggggattcga ccatgcccaa gaagaagagg aaggtgtcca atttactgac
     2521 cgtacaccaa aatttgcctg cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa
     2581 cctgatggac atgttcaggg atcgccaggc gttttctgag catacctgga aaatgcttct
     2641 gtccgtttgc cggtcgtggg cggcatggtg caagttgaat aaccggaaat ggtttcccgc
     2701 agaacctgaa gatgttcgcg attatcttct atatcttcag gcgcgcggtc tggcagtaaa
     2761 aactatccag caacatttgg gccagctaaa catgcttcat cgtcggtccg ggctgccacg
     2821 accaagtgac agcaatgctg tttcactggt tatgcggcgg atccgaaaag aaaacgttga
     2881 tgccggtgaa cgtgcaaaac aggctctagc gttcgaacgc actgatttcg accaggttcg
     2941 ttcactcatg gaaaatagcg atcgctgcca ggatatacgt aatctggcat ttctggggat
     3001 tgcttataac accctgttac gtatagccga aattgccagg atcagggtta aagatatctc
     3061 acgtactgac ggtgggagaa tgttaatcca tattggcaga acgaaaacgc tggttagcac
     3121 cgcaggtgta gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc gatggatttc
     3181 cgtctctggt gtagctgatg atccgaataa ctacctgttt tgccgggtca gaaaaaatgg
     3241 tgttgccgcg ccatctgcca ccagccagct atcaactcgc gccctggaag ggatttttga
     3301 agcaactcat cgattgattt acggcgctaa ggatgactct ggtcagagat acctggcctg
     3361 gtctggacac agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg gagtttcaat
     3421 accggagatc atgcaagctg gtggctggac caatgtaaat attgtcatga actatatccg
     3481 taacctggat agtgaaacag gggcaatggt gcgcctgctg gaagatggcg attagccatt
     3541 aacgcgcgtt gctgtcggat ccgcagatcc cggccagata ccgatgctgc cgcagcaaaa
     3601 gcaggagcag atgccgccgt cgcaggcgaa gatgtcgcag acggaggagg cgatgctgcc
     3661 ggcggaggag gcgaagtaag tagagggctg ggctgggctg tggggggtgt ggggtgcggg
     3721 actgggcagt ctgggagtcc ctctcaccac ttttcttacc tttctaggat gctgccgtcg
     3781 ccgccgctca tacaccataa ggtgtaaaaa atactagatg cacagaatag caagtccatc
     3841 aaaactcctg cgtgagaatt ttaccagact tcaagagcat ctcgccacat cttgaaaaat
     3901 gccaccgtcc gatgaaaaac aggagcctgc taaggaacaa tgccacctgt caataaatgt
     3961 tgaaaactca tcccattcct gcctcttggt ccttgggctt ggggaggggt gcgcggatgt
     4021 ggttagggaa catgactggt caaatgggaa gggcttcaaa agaattccca atattgacta
     4081 ccaagccacc tgtacagatc tgcctgcagg catgcaagct tggcactggc cgtcgtttta
     4141 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     4201 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     4261 cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     4321 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     4381 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     4441 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     4501 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     4561 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     4621 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4681 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4741 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4801 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4861 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4921 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     4981 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5041 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5101 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5161 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5221 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5281 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5341 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5401 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5461 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5521 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5581 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5641 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5701 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5761 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5821 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5881 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     5941 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6001 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6061 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6121 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6181 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6241 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6301 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6361 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6421 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6481 acgaccgagc gcagcgagtc agtgagcgag ga
//
