LOCUS       sequence-302080-        9772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of HO endonuclease in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9772)
  AUTHORS   Gnugge R, Symington LS
  TITLE     Efficient DNA double-strand break formation at single or multiple 
            defined sites in the Saccharomyces cerevisiae genome.
  JOURNAL   Nucleic Acids Res. 2020 Oct 14. pii: 5923424. doi: 
            10.1093/nar/gkaa833.
  PUBMED    33053188
REFERENCE   2  (bases 1 to 9772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Oct 14. pii: 5923424. doi: 10.1093/nar/gkaa833."
COMMENT     Sequence Label: pRG713_lexO-HO_LexA-TF_URA3MX
FEATURES             Location/Qualifiers
     source          1..9772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(340..537)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(1356..1699)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     1787..1809
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1801..1818
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1802..1818
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1825..1844
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1825..1843
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3410..3421
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4025..4048
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      4098..4347
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             join(5017..5268,5269..5622)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     5560..5581
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             5641..6576
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             5689..6579
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     terminator      7043..7292
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(7455..7473)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7494..7510)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7494..7510)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7507..7529)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7518..7534
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(7844..7861)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8015..8603)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8095..8114)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8782..9573,9574..9642))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9405..9424
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9643..9747)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcgaaa gaacgcggca ttagcaacat ttgcgaagcg aacgattttg cgaactgcta
       61 ttaattaact ctggcgcgcc actacttttt ggaactcttg ttgttctttg gagttcaatg
      121 cgtccatctt tacagtcctg tcttattgtt cttgatttgt gccccgtaaa atactgttac
      181 ttggttctgg cgaggtattg gatagttcct ttttataaag gccatgaagg tttttctttc
      241 caattttttt tttttcgtca ttatagaaat cattacgacc gagattcccg gcatatgcgt
      301 tttcgacact ggatggcggc gttagtatcg aatcgacagc agtatagcga ccagcattca
      361 catacgattg acgcatgata ttactttctg cgcacttaac ttcgcatctg ggcagatgat
      421 gtcgaggcga aaaaaaatat aaatcacgct aacatttgat taaaatagaa caactacaat
      481 ataaaaaaac tatacaaatg acaagttctt gaaaacaaga atctttttat tgtcagtact
      541 gattataatt ggccagtctt tttcaaataa gcattccaac cagcatctct atacctttta
      601 ccttcaatat ctggatctct tcctttacca aacaatcctc taccaacaat gataatatca
      661 gttccagtgc taacaacttc atcaacagtt ctatattgtt gtcctaatcc atcaccttta
      721 tcatctaatc caactccagg tgtcataata agccaatcaa atccttcttc ttgtccaccc
      781 atatcacgtt gggcaataaa tccaataaca aattccttat cggatttagc aatttcaaca
      841 gttttttgag aatattctcc ataagctaat gatcccactg atgataattc agctaacatc
      901 aataaccctc ttggctcttg gttggtggtg gtttctttag ctccctgttt taatccttca
      961 actactccat tcccagtgac accatgagca ttagtaatat ctgcccaact actaatttta
     1021 taaactccac caatatattg tttcttcacg gtattaccaa tatcagcaaa ttttctatct
     1081 tcaaaaatca taaattgatg tttacgtgaa agttctaata atggttcaat agtggattca
     1141 taggaaaaat cattgattat atcaatatga gtcttgatta agcatacata aggacccaat
     1201 ttatcaatta attcaagaaa ttccttagtg gtatcaacat caattgatgc acataaattg
     1261 gttttcttca gttccattaa tcgaaataat cgttgtgcta ctggtgaggc atgagtttct
     1321 gctctctcac tataggtctt agtgttgact gtcatggttg tttatgttcg gatgtgatgt
     1381 gagaactgta tcctagcaag attttaaaag gaagtatatg aaagaagaac ctcagtggca
     1441 aatcctaacc ttttatattt ctctacaggg gcgcggcgtg gggacaattc aacgcgtctg
     1501 tgaggggagc gtttccctgc tcgcaggtcc gcagcgagga gccgtaattt ttgcttcgcg
     1561 ccgtgcggcc atcaaaatgt atggatgcaa atgattatac atggggatgt atgggctaaa
     1621 tgtacgggcg acagtcacat catgcccctg agctgcgcac gtcaagactg tcaaggaggg
     1681 tattctgggc ctccatgtcg ctggccgggt gacccggcgg ggacgaggca agctaaaccg
     1741 atcggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     1801 tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt ggagaaacag
     1861 aataatatat aaacctgtat aatataacct tgaagactat atttcttttc gcggccgcct
     1921 atagggcgaa ttggagctcc ctaggtgctg tatatactca cagcataact gtatatacac
     1981 ccagggtcta ggtgctgtat atactcacag cataactgta tatacaccca gggtctaggt
     2041 gctgtatata ctcacagcat aactgtatat acacccaggg tctaggtgct gtatatactc
     2101 acagcataac tgtatataca cccagggtct agagcatgtg ctctgtatgt atataaaact
     2161 cttgttttct tcttttctct tctttcctta tacattagga cctttgcagc ataaattact
     2221 atacttctat actagtggat cccccgggct gcaggaattc atgctttctg aaaacacgac
     2281 tattctgatg gctaacggtg aaattaaaga catcgcaaac gtcacggcta actcttacgt
     2341 tatgtgcgca gatggctccg ctgcccgcgt cataaatgtc acacagggct atcagaaaat
     2401 ctataatata cagcaaaaaa ccaaacacag agcttttgaa ggtgaacctg gtaggttaga
     2461 tcccaggcgt agaacagttt atcagcgtct tgcattacaa tgtactgcag gtcataaatt
     2521 gtcagtcagg gtccctacca aaccactgtt ggaaaaaagt ggtagaaatg ccaccaaata
     2581 taaagtgaga tggagaaatc tgcagcaatg tcagacgctt gatggtagga taataataat
     2641 tccaaaaaac catcataaga cattcccaat gacagttgaa ggtgagtttg ccgcaaaacg
     2701 cttcatagaa gaaatggagc gctctaaagg agaatatttc aactttgaca ttgaagttag
     2761 agatttggat tatcttgatg ctcaattgag aatttctagc tgcataagat ttggtccagt
     2821 actcacagga aatggtgttt tatctaaatt tctcactgga cgtagtgacc ttgtaactcc
     2881 tgctgtaaaa agtatggctt ggatgcttgg tctgtggtta ggtgacggta caacaaaaga
     2941 gccagaaatc tcagtagata gcttggatcc taagctaatg gagagtttaa gagaaaatgc
     3001 gaaaatctgg ggtctctacc ttacggtttg tgacgatcac gttccgctac gtgccaaaca
     3061 tgtaaggctt cattatggag atggtccaga tgaaaacagg aagacaagga atttgaggaa
     3121 aaataatcca ttctggaaag ctgtcacaat tttaaagttt aaaagggatc ttgatggaga
     3181 gaagcaaatc cctgaattta tgtacggcga gcatatagaa gttcgtgaag cattcttagc
     3241 cggcttgatc gactcagatg ggtacgttgt gaaaaagggc gaaggccctg aatcttataa
     3301 aatagcaatt caaactgttt attcatccat tatggacgga attgtccata tttcaagatc
     3361 tcttggtatg tcagctactg tgacgaccag gtcagctagg gaggaaatca ttgaaggaag
     3421 aaaagtccaa tgtcaattta catacgactg taatgttgct gggggaacaa ctttacagaa
     3481 tgttttgtca tattgtcgaa gtggtcacaa aacaagagaa gttccgccaa ttataaaaag
     3541 ggaacccgta tatttcagct tcacggatga tttccagggt gagagtactg tatatgggct
     3601 tacgatagaa ggccataaaa atttcttgct tggcaacaaa atagaagtga aatcatgtcg
     3661 aggctgctgt gtgggagaac agcataaaat atcacaaaaa aagaatctaa aacactgtgt
     3721 tgcttgtccc agaaagggaa tcaagtattt ttataaagat tggagtggta aaaatcgagt
     3781 atgtgctaga tgctatggaa gatacaaatt cagcggtcat cactgtataa attgcaagta
     3841 tgtaccagaa gcacgtgaag tgaaaaaggc aaaagacaaa ggcgaaaaat tgggcattac
     3901 gcccgaaggt ttgccagtta aaggaccaga gtgtataaaa tgtggcggaa tcttacagtt
     3961 tgatgctgtc cgcgggcctc ataagagttg tggtaacaac gcaggtgcgc gcatctgcga
     4021 tatcgactac aaggatgacg acgacaagaa gctttaacta actaacctcg agacaggccc
     4081 cttttccttt gtctatatca tgtaattagt tatgtcacgc ttacattcac gccctcctcc
     4141 cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta
     4201 ttttttttaa tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt
     4261 tttctgtaca aacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt
     4321 ttgggacgct cgaaggcttt aatttgcaag gttcgcagtt tacactctca tcgtcgctct
     4381 catcatcgct tccgttgttg ttttccttag tagcgtctgc ttggtaccca gcttcggccg
     4441 ctctagagaa gcgggtaagc tgccacagca attaatgcac aacatttaac ctacattctt
     4501 ccttatcgga tcctcaaaac ccttaaaaac atatgcctca ccctaacata ttttccaatt
     4561 aaccctcaat atttctctgt cacccggcct ctattttcca ttttcttctt tacccgccac
     4621 gcgttttttt ctttcaaatt tttttcttcc ttcttctttt tcttccacgt cctcttgcat
     4681 aaataaataa accgttttga aaccaaactc gcctctctct ctcctttttg aaatattttt
     4741 gggtttgttt gatcctttcc ttcccaatct ctcttgttta atatatattc atttatatca
     4801 cgctctcttt ttatcttcct ttttttcctc tctcttgtat tcttccttcc cctttctact
     4861 caaaccaaga agaaaaagaa aaggtcaatc tttgttaaag aataggatct tctactacat
     4921 cagcttttag atttttcacg cttactgctt ttttcttccc aagatcgaaa atttactgaa
     4981 ttaacaacta gtggatcccc cgggctgcag gaattcatga aagcgttaac ggccaggcaa
     5041 caagaggtgt ttgatctcat ccgtgatcac atcagccaga caggtatgcc gccgacgcgt
     5101 gcggaaatcg cgcagcgttt ggggttccgt tccccaaacg cggctgaaga acatctgaag
     5161 gcgctggcac gcaaaggcgt tattgaaatt gtttccggcg catcacgcgg gattcgtctg
     5221 ttgcaggaag aggaagaagg gttgccgctg gtaggtcgtg tggctgccgg tgaaccactt
     5281 ctggcgcaac agcatattga aggtcattat caggtcgatc cttccttatt caagccgaat
     5341 gctgatttcc tgctgcgcgt cagcgggatg tcgatgaaag acatcggcat tatggatggt
     5401 gacttgctgg cagtgcataa aactcaggat gtacgtaacg gtcaggtcgt tgtcgcacgt
     5461 attgatgacg aggttaccgt taagcgcctg aaaaaacagg gcaataaagt cgaactgttg
     5521 ccagaaaata gcgagtttaa accaattgtc gtagatcttc gtcagcagag cttcaccatt
     5581 gaagggctgg cggttggggt tattcgcaac ggcgactggc tgggttctaa ggatatctct
     5641 gctggagaca tgagagctgc caacctttgg ccaagcccgc tcatgatcaa acgctctaag
     5701 aagaacagcc tggccttgtc cctgacggcc gaccagatgg tcagtgcctt gttggatgct
     5761 gagcccccca tactctattc cgagtatgat cctaccagac ccttcagtga agcttcgatg
     5821 atgggcttac tgaccaacct ggcagacagg gagctggttc acatgatcaa ctgggcgaag
     5881 agggtgccag gctttgtgga tttgaccctc catgatcagg tccaccttct agaatgtgcc
     5941 tggttagaga tcctgatgat tggtctcgtc tggcgctcca tggagcaccc agtgaagcta
     6001 ctgtttgctc ctaacttgct cttggacagg aaccagggaa aatgtgtaga gggcatggtg
     6061 gagatcttcg acatgctgct ggctacatca tctcggttcc gcatgatgaa tctgcaggga
     6121 gaggagtttg tgtgcctcaa atctattatt ttgcttaatt ctggagtgta cacatttctg
     6181 tccagcaccc tgaagtctct ggaagagaag gaccatatcc accgagtcct ggacaagatc
     6241 acagacactt tgatccacct gatggccaag gcaggcctga ccctgcagca gcagcaccag
     6301 cggctggccc agctcctcct catcctctcc cacatcaggc acatgagtaa caaaggcatg
     6361 gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc tctatgacct gctgctggag
     6421 atgctggacg cccaccgcct acatgcgccc actagccgtg gaggggcatc cgtggaggag
     6481 acggaccaaa gccacttggc cactgcgggc tctacttcat cgcattcctt gcaaaagtat
     6541 tacatcacgg gggaggcaga gggtttccct gccacagtcg atatcatgca ggatctgccg
     6601 ggcaacgata acagcaccgc gggcgaattt ccaggtatta ctttgagaat ccaagaaact
     6661 gatatgttgt ataaaggtga tactttgtat ttggattggt tggaagatgg tattgctgaa
     6721 ttggttttcg atgctccagg ttctgttaat aaattggata ctgctgttgc ttctttgggt
     6781 gaagctattg gtgttttgga acaacaatct gatttgattt gggaaacttt gactgttaaa
     6841 gatgctaaag ttaatttcga ttctggtttg gaaaaattcg aagaagctat tccatctgct
     6901 gatgatttcg atccagttgc tgaaagaaga tcctctggtg aatttagagc tgaaagacat
     6961 tctggtggta ctgatttgtg tttcaagctt taaacgcgtt taaagcttat cctcgagaca
     7021 ggcccctttt cctttgtcta tatcatgtaa ttagttatgt cacgcttaca ttcacgccct
     7081 cctcccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct
     7141 atttattttt tttaatagtt atgttagtat taagaacgtt atttatattt caaatttttc
     7201 ttttttttct gtacaaacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga
     7261 aggttttggg acgctcgaag gctttaattt gcaaggttcg cagtttacac tctcatcgtc
     7321 gctctcatca tcgcttccgt tgttgttttc cttagtagcg tctgcttggt acccagcttc
     7381 ggccgtattt acagataacg atgtcgttat tatatatata tatatatatt gtcacttgta
     7441 cccagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag
     7501 ctgtttcctg tgtgaaattg ttatccgctc acaacagctg gatgaattga atagctttat
     7561 ggaccctgaa accacagcca cattaacctt ctttgatggt caaaacttat ccttcaccat
     7621 aaatatgcct cgcaaaaaag gtaattaaca tatatagaat tacattattt atgaaatatc
     7681 atcactatct cttagcatct ttaatccttt tctacatcag ataacttcgg tttgttatca
     7741 tcgtctgtat tgtcatcaat tggcgcgcct ctccctgcag gcattaatga atcggccaac
     7801 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7861 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7921 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7981 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8041 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8101 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8161 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8221 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8281 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8341 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8401 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     8461 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8521 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8581 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttgtttaaac ctacggggtc
     8641 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8701 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8761 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8821 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8881 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8941 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9001 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9061 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9121 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9181 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatggttg tcagaagtaa
     9241 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9301 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9361 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9421 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9481 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9541 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9601 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9661 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9721 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ct
//
