LOCUS       sequence-302082-        9400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of AscI endonuclease in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   Gnugge R, Symington LS
  TITLE     Efficient DNA double-strand break formation at single or multiple 
            defined sites in the Saccharomyces cerevisiae genome.
  JOURNAL   Nucleic Acids Res. 2020 Oct 14. pii: 5923424. doi: 
            10.1093/nar/gkaa833.
  PUBMED    33053188
REFERENCE   2  (bases 1 to 9400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Oct 14. pii: 5923424. doi: 10.1093/nar/gkaa833."
COMMENT     Sequence Label: pRG714_lexO-AscI_LexA-TF_URA3MX
FEATURES             Location/Qualifiers
     source          1..9400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(340..537)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(1356..1699)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     1787..1809
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1801..1818
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1802..1818
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1825..1844
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1825..1843
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3626..3646
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3653..3676
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      3726..3975
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             join(4645..4896,4897..5250)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     5188..5209
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             5269..6204
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             5317..6207
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     terminator      6671..6920
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(7083..7101)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7122..7138)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7122..7138)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7135..7157)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7146..7162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(7472..7489)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7643..8231)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7723..7742)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8410..9201,9202..9270))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9033..9052
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9271..9375)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcgaaa gaacgcggca ttagcaacat ttgcgaagcg aacgattttg cgaactgcta
       61 ttaattaact ctggcgcgcc actacttttt ggaactcttg ttgttctttg gagttcaatg
      121 cgtccatctt tacagtcctg tcttattgtt cttgatttgt gccccgtaaa atactgttac
      181 ttggttctgg cgaggtattg gatagttcct ttttataaag gccatgaagg tttttctttc
      241 caattttttt tttttcgtca ttatagaaat cattacgacc gagattcccg gcatatgcgt
      301 tttcgacact ggatggcggc gttagtatcg aatcgacagc agtatagcga ccagcattca
      361 catacgattg acgcatgata ttactttctg cgcacttaac ttcgcatctg ggcagatgat
      421 gtcgaggcga aaaaaaatat aaatcacgct aacatttgat taaaatagaa caactacaat
      481 ataaaaaaac tatacaaatg acaagttctt gaaaacaaga atctttttat tgtcagtact
      541 gattataatt ggccagtctt tttcaaataa gcattccaac cagcatctct atacctttta
      601 ccttcaatat ctggatctct tcctttacca aacaatcctc taccaacaat gataatatca
      661 gttccagtgc taacaacttc atcaacagtt ctatattgtt gtcctaatcc atcaccttta
      721 tcatctaatc caactccagg tgtcataata agccaatcaa atccttcttc ttgtccaccc
      781 atatcacgtt gggcaataaa tccaataaca aattccttat cggatttagc aatttcaaca
      841 gttttttgag aatattctcc ataagctaat gatcccactg atgataattc agctaacatc
      901 aataaccctc ttggctcttg gttggtggtg gtttctttag ctccctgttt taatccttca
      961 actactccat tcccagtgac accatgagca ttagtaatat ctgcccaact actaatttta
     1021 taaactccac caatatattg tttcttcacg gtattaccaa tatcagcaaa ttttctatct
     1081 tcaaaaatca taaattgatg tttacgtgaa agttctaata atggttcaat agtggattca
     1141 taggaaaaat cattgattat atcaatatga gtcttgatta agcatacata aggacccaat
     1201 ttatcaatta attcaagaaa ttccttagtg gtatcaacat caattgatgc acataaattg
     1261 gttttcttca gttccattaa tcgaaataat cgttgtgcta ctggtgaggc atgagtttct
     1321 gctctctcac tataggtctt agtgttgact gtcatggttg tttatgttcg gatgtgatgt
     1381 gagaactgta tcctagcaag attttaaaag gaagtatatg aaagaagaac ctcagtggca
     1441 aatcctaacc ttttatattt ctctacaggg gcgcggcgtg gggacaattc aacgcgtctg
     1501 tgaggggagc gtttccctgc tcgcaggtcc gcagcgagga gccgtaattt ttgcttcgcg
     1561 ccgtgcggcc atcaaaatgt atggatgcaa atgattatac atggggatgt atgggctaaa
     1621 tgtacgggcg acagtcacat catgcccctg agctgcgcac gtcaagactg tcaaggaggg
     1681 tattctgggc ctccatgtcg ctggccgggt gacccggcgg ggacgaggca agctaaaccg
     1741 atcggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     1801 tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt ggagaaacag
     1861 aataatatat aaacctgtat aatataacct tgaagactat atttcttttc gcggccgcct
     1921 atagggcgaa ttggagctcc ctaggtgctg tatatactca cagcataact gtatatacac
     1981 ccagggtcta ggtgctgtat atactcacag cataactgta tatacaccca gggtctaggt
     2041 gctgtatata ctcacagcat aactgtatat acacccaggg tctaggtgct gtatatactc
     2101 acagcataac tgtatataca cccagggtct agagcatgtg ctctgtatgt atataaaact
     2161 cttgttttct tcttttctct tctttcctta tacattagga cctttgcagc ataaattact
     2221 atacttctat actagtggat cccccgggct gcaggaattc atgattgaat ttccagagta
     2281 cagagattct tctgctgctc caaaaatctc cgatttggaa agattgatgg acaggtcttt
     2341 gaccaactct caatatgttg atggtgctaa cgctgctaga ttattgggta ctttcatcag
     2401 atccttcaga tccgttattg gttctgctga agaatctgct actagagcta atttggttga
     2461 agctcatgat gaagctaagt tgttcggttt gatgttgtct gctggtttcg atttgatctg
     2521 taacgctgaa tacgttcacg gtagattggt taacaacaag tggatctatt gtcacagagg
     2581 tggtgaacca gcagttgctt attactcttt cttgaaacaa tgtccaagat gctgcttgga
     2641 tagaggtttg gaaggtagat tgtctggtgc tcaacataag ccaacttctc atcatattgg
     2701 tgaaattacc accgttgcta ttgccttgtt gttgcaatta gttgctgctg ctaacgaaaa
     2761 cccattcgaa attgctacta tcaccaagca atcccatgat gttgatgcta ttggtttcag
     2821 agatgacttg ttggtcttgt tcgaaatcaa ggcttctcca atggtttctt tcccattggt
     2881 tactgaattg gaagaaccta tgttgcaaga aggtccagac ggtccagttg agtatagaca
     2941 acattctttg gttgacttga cgttgcaggg tagagaattt gctgttgcta ttccacatgc
     3001 tgaaacagct attccattgg gtgaaagaga aggcgaatct tggccatacg aaccattgat
     3061 tgattacttt tctgttccag ctaatgccgc ttcttacttg caagcctgga ttgaattata
     3121 tgctgcttac agaactccca agactcaaag agctggtaga actgctgctt tggcttattt
     3181 ggttaatggt tggggtgacg aaatcgattc taacaaaaca aaaccaggtt tgggtagaac
     3241 cgatgatgtt aagaaaggta cttaccagtt gttgaagttc ggttcctatt acagagatga
     3301 tgctgcatca gttccagtta gaggtgcttt ggttgctaac ttagatcctt tgtttttgag
     3361 gccaggttac atcgatggtt tgtctgatgt tagatggggt catggtagag atttcacttt
     3421 ggaagaaggt gagtacagaa ttgctgaagg ttctttgaga cacttgtacg atgctatttt
     3481 ggctttcaat gaccctttgt tgaatgaccc attgctgcaa gaaatctttg atttgggtgc
     3541 tgtcgaaaga aagttggcta atggtgattt ggaagccttg ttggataagt ggattgctag
     3601 accagaaata gttttggacc catctccaaa gaagaagaga aaagttgata tcgactacaa
     3661 ggatgacgac gacaagaagc tttaactaac taacctcgag acaggcccct tttcctttgt
     3721 ctatatcatg taattagtta tgtcacgctt acattcacgc cctcctccca catccgctct
     3781 aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt ttttttaata
     3841 gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaaa
     3901 cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg
     3961 aaggctttaa tttgcaaggt tcgcagttta cactctcatc gtcgctctca tcatcgcttc
     4021 cgttgttgtt ttccttagta gcgtctgctt ggtacccagc ttcggccgct ctagagaagc
     4081 gggtaagctg ccacagcaat taatgcacaa catttaacct acattcttcc ttatcggatc
     4141 ctcaaaaccc ttaaaaacat atgcctcacc ctaacatatt ttccaattaa ccctcaatat
     4201 ttctctgtca cccggcctct attttccatt ttcttcttta cccgccacgc gtttttttct
     4261 ttcaaatttt tttcttcctt cttctttttc ttccacgtcc tcttgcataa ataaataaac
     4321 cgttttgaaa ccaaactcgc ctctctctct cctttttgaa atatttttgg gtttgtttga
     4381 tcctttcctt cccaatctct cttgtttaat atatattcat ttatatcacg ctctcttttt
     4441 atcttccttt ttttcctctc tcttgtattc ttccttcccc tttctactca aaccaagaag
     4501 aaaaagaaaa ggtcaatctt tgttaaagaa taggatcttc tactacatca gcttttagat
     4561 ttttcacgct tactgctttt ttcttcccaa gatcgaaaat ttactgaatt aacaactagt
     4621 ggatcccccg ggctgcagga attcatgaaa gcgttaacgg ccaggcaaca agaggtgttt
     4681 gatctcatcc gtgatcacat cagccagaca ggtatgccgc cgacgcgtgc ggaaatcgcg
     4741 cagcgtttgg ggttccgttc cccaaacgcg gctgaagaac atctgaaggc gctggcacgc
     4801 aaaggcgtta ttgaaattgt ttccggcgca tcacgcggga ttcgtctgtt gcaggaagag
     4861 gaagaagggt tgccgctggt aggtcgtgtg gctgccggtg aaccacttct ggcgcaacag
     4921 catattgaag gtcattatca ggtcgatcct tccttattca agccgaatgc tgatttcctg
     4981 ctgcgcgtca gcgggatgtc gatgaaagac atcggcatta tggatggtga cttgctggca
     5041 gtgcataaaa ctcaggatgt acgtaacggt caggtcgttg tcgcacgtat tgatgacgag
     5101 gttaccgtta agcgcctgaa aaaacagggc aataaagtcg aactgttgcc agaaaatagc
     5161 gagtttaaac caattgtcgt agatcttcgt cagcagagct tcaccattga agggctggcg
     5221 gttggggtta ttcgcaacgg cgactggctg ggttctaagg atatctctgc tggagacatg
     5281 agagctgcca acctttggcc aagcccgctc atgatcaaac gctctaagaa gaacagcctg
     5341 gccttgtccc tgacggccga ccagatggtc agtgccttgt tggatgctga gccccccata
     5401 ctctattccg agtatgatcc taccagaccc ttcagtgaag cttcgatgat gggcttactg
     5461 accaacctgg cagacaggga gctggttcac atgatcaact gggcgaagag ggtgccaggc
     5521 tttgtggatt tgaccctcca tgatcaggtc caccttctag aatgtgcctg gttagagatc
     5581 ctgatgattg gtctcgtctg gcgctccatg gagcacccag tgaagctact gtttgctcct
     5641 aacttgctct tggacaggaa ccagggaaaa tgtgtagagg gcatggtgga gatcttcgac
     5701 atgctgctgg ctacatcatc tcggttccgc atgatgaatc tgcagggaga ggagtttgtg
     5761 tgcctcaaat ctattatttt gcttaattct ggagtgtaca catttctgtc cagcaccctg
     5821 aagtctctgg aagagaagga ccatatccac cgagtcctgg acaagatcac agacactttg
     5881 atccacctga tggccaaggc aggcctgacc ctgcagcagc agcaccagcg gctggcccag
     5941 ctcctcctca tcctctccca catcaggcac atgagtaaca aaggcatgga gcatctgtac
     6001 agcatgaagt gcaagaacgt ggtgcccctc tatgacctgc tgctggagat gctggacgcc
     6061 caccgcctac atgcgcccac tagccgtgga ggggcatccg tggaggagac ggaccaaagc
     6121 cacttggcca ctgcgggctc tacttcatcg cattccttgc aaaagtatta catcacgggg
     6181 gaggcagagg gtttccctgc cacagtcgat atcatgcagg atctgccggg caacgataac
     6241 agcaccgcgg gcgaatttcc aggtattact ttgagaatcc aagaaactga tatgttgtat
     6301 aaaggtgata ctttgtattt ggattggttg gaagatggta ttgctgaatt ggttttcgat
     6361 gctccaggtt ctgttaataa attggatact gctgttgctt ctttgggtga agctattggt
     6421 gttttggaac aacaatctga tttgatttgg gaaactttga ctgttaaaga tgctaaagtt
     6481 aatttcgatt ctggtttgga aaaattcgaa gaagctattc catctgctga tgatttcgat
     6541 ccagttgctg aaagaagatc ctctggtgaa tttagagctg aaagacattc tggtggtact
     6601 gatttgtgtt tcaagcttta aacgcgttta aagcttatcc tcgagacagg ccccttttcc
     6661 tttgtctata tcatgtaatt agttatgtca cgcttacatt cacgccctcc tcccacatcc
     6721 gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt
     6781 taatagttat gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt
     6841 acaaacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac
     6901 gctcgaaggc tttaatttgc aaggttcgca gtttacactc tcatcgtcgc tctcatcatc
     6961 gcttccgttg ttgttttcct tagtagcgtc tgcttggtac ccagcttcgg ccgtatttac
     7021 agataacgat gtcgttatta tatatatata tatatattgt cacttgtacc cagcttttgt
     7081 tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg
     7141 tgaaattgtt atccgctcac aacagctgga tgaattgaat agctttatgg accctgaaac
     7201 cacagccaca ttaaccttct ttgatggtca aaacttatcc ttcaccataa atatgcctcg
     7261 caaaaaaggt aattaacata tatagaatta cattatttat gaaatatcat cactatctct
     7321 tagcatcttt aatccttttc tacatcagat aacttcggtt tgttatcatc gtctgtattg
     7381 tcatcaattg gcgcgcctct ccctgcaggc attaatgaat cggccaacgc gcggggagag
     7441 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7501 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7561 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7621 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7681 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7741 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7801 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7861 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7921 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7981 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8041 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8101 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8161 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8221 aaggatctca agaagatcct ttgatctttt gtttaaacct acggggtctg acgctcagtg
     8281 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8341 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8401 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8461 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8521 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8581 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8641 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8701 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     8761 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     8821 caaaaaagcg gttagctcct tcggtcctcc gatggttgtc agaagtaagt tggccgcagt
     8881 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8941 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9001 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9061 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9121 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9181 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9241 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9301 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9361 aataggggtt ccgcgcacat ttccccgaaa agtgccacct
//
