LOCUS       sequence-30210-e         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Morimoto-Tomita M, Uchimura K, Bistrup A, Lum DH, Egeblad M, 
            Boudreau N, Werb Z, Rosen SD
  TITLE     Sulf-2, a proangiogenic heparan sulfate endosulfatase, is 
            upregulated in breast cancer.
  JOURNAL   Neoplasia. 2005 Nov . 7(11):1001-10.
  PUBMED    16331886
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neoplasia. 
            2005 Nov . 7(11):1001-10."
COMMENT     Sequence Label: f2H ∂HD (272)
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     92..151
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             194..217
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagccac catggagaca gacacactcc tgctatgggt
      121 actgctgctc tgggttccag gttccactgg tgacgcggcc cagccggcca ggcgcgcgcg
      181 ccgtacgaag cttgattaca aggatgacga cgataagcca gcagcattcc tgtcgcacca
      241 ccgcctgaaa ggcaggtttc agagggaccg caggaacatc cgccccaaca tcatcctggt
      301 gctgacggac gaccaggatg tggagctggg ttccatgcag gtgatgaaca agacccggcg
      361 catcatggag cagggcgggg cgcacttcat caacgccttc gtgaccacac ccatgtgctg
      421 cccctcacgc tcctccatcc tcaccggcaa gtacgtccac aaccacaaca cctacaccaa
      481 caatgagaac tgctcctcgc cctcctggca ggcacagcac gagagccgca cctttgccgt
      541 gtacctcaat agcactggct accggacagc tttcttcggg aagtatctta atgaatacaa
      601 cggctcctac gtgccacccg gctggaagga gtgggtcgga ctccttaaaa actcccgctt
      661 ttataactac acgctgtgtc
//
