LOCUS       sequence-30212-e         656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Valastyan S, Reinhardt F, Benaich N, Calogrias D, Szasz AM, Wang ZC,
            Brock JE, Richardson AL, Weinberg RA
  TITLE     A pleiotropically acting microRNA, miR-31, inhibits breast cancer 
            metastasis.
  JOURNAL   Cell. 2009 Jun 12. 137(6):1032-46.
  PUBMED    19524507
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jun 12. 137(6):1032-46."
COMMENT     Sequence Label: pIS1-miR-31 site
FEATURES             Location/Qualifiers
     source          1..656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    200..321
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(211..230)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     265..284
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        454..558
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 actgaatttg tcaaagtaaa aggtcttcat ttttcgcaag aagatgcacc tgatgaaatg
       61 ggaaaatata tcaaatcgtt cgttgagcga gttctcaaaa atgaacaata attctaggag
      121 ctcaggcaag atgctggcat agctaccggt ctcgatatca ctactagtgt tctagagcgg
      181 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
      241 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
      301 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
      361 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat
      421 aaggatccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      481 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      541 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
      601 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaaga
//
