LOCUS       sequence-30215-e         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Schwer B, Ren S, Pietschmann T, Kartenbeck J, Kaehlcke K, 
            Bartenschlager R, Yen TS, Ott M
  TITLE     Targeting of hepatitis C virus core protein to mitochondria through 
            a novel C-terminal localization motif.
  JOURNAL   J Virol. 2004 Aug . 78(15):7958-68.
  PUBMED    15254168
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2004 Aug . 78(15):7958-68."
COMMENT     Sequence Label: CMV-FLAG-Core-R (pMO29)
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..121
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gcgtttaaac ttaagcttgc caccatggac tacaaggacg acgatgacaa
      121 gatgagcacg aatcctaaac ctcaaagaaa aaccaaacgt aacaccaacc gccgcccaca
      181 ggacgtcaag ttcccgggcg gtggtcagat cgtcggtgga gtttacctgt tgccgcgcag
      241 gggccccagg ttgggtgtgc gcgcgactag gaagacttcc gagcggtcgc aacctcgtgg
      301 aaggcgacaa cctatcccca aggctcgcca gcccgagggt agggcctggg ctcagcccgg
      361 gtacccctgg cccctctatg gcaatgaggg cttggggtgg gcaggatggc tcctgtcacc
      421 ccgtggctct cggcctagtt ggggccccac ggacccccgg cgtaggtcgc gcaatttggg
      481 taaggtcatc gataccctca cgtgcggctt cgccgatctc atggggtacc ttccgctcgt
      541 cggcgccccc ctagggggcg ctgccagggc cctggcgcat ggcgtccggg ttctggagga
      601 cggcgtgaac tatgcaacag ggaatctgcc cggttgctcc ttttctatct tccttttggc
      661 tttgctgtcc tgtttgacca tcccagc
//
