LOCUS       sequence-302176-         423 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-TNFRSF19-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..423
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             339..347
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gaatctggcg attgcagaca gcaagagttc cgggacagat ccggcaactg cgtgccctgt
       61 aatcagtgtg gccctggcat ggaactgagc aaagagtgtg gcttcggata cggcgaggat
      121 gcccagtgtg tgacctgccg gctgcacaga ttcaaagagg actggggctt ccagaagtgc
      181 aagccttgtc tggactgcgc cgtggtcaac agattccaga aggccaattg cagcgccacc
      241 tccgatgcca tctgtggcga ttgtctgccc ggcttctaca gaaagaccaa gctcgtggga
      301 ttccaggaca tggaatgcgt gccatgtggc gaccctccac ctccttatga acctcactgc
      361 gccagcaaag tgaacctggt caagatcgcc agcaccgcca gctctccaag agacacagct
      421 ctt
//
