LOCUS       sequence-30225-e         669 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Hirschey MD, Shimazu T, Goetzman E, Jing E, Schwer B, Lombard DB, 
            Grueter CA, Harris C, Biddinger S, Ilkayeva OR, Stevens RD, Li Y, 
            Saha AK, Ruderman NB, Bain JR, Newgard CB, Farese RV, Alt FW, Kahn 
            CR, Verdin E
  TITLE     SIRT3 regulates mitochondrial fatty-acid oxidation by reversible 
            enzyme deacetylation.
  JOURNAL   Nature. 2010 Mar 4. 464(7285):121-5.
  PUBMED    20203611
REFERENCE   2  (bases 1 to 669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Mar 4. 464(7285):121-5."
COMMENT     Sequence Label: pcDNA3.1mLCAD_K42A_FLAG
FEATURES             Location/Qualifiers
     source          1..669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      121 caccatggct gcgcgcctgc tcctccgctc cctgcgcgtc ctgaaagccc gctcggcgcc
      181 acgcccgccg ccctccgccc gatgttctca ttctggagca gaagcgcgtc tagaaactcc
      241 ttctgcagca aaattaactg acgttggaat caggagaatc ttttcctcgg agcatgacat
      301 tttccgggag agtgtaagga agtttttcca agaagaagtg attcctcacc acacagaatg
      361 ggagaaagct ggagaagtga gtagagaggt ctgggaaaaa gctggcaagc agggcttgct
      421 tggcatcaac atcgcagaga aacatggcgg cattggtggg gacttgctct caacagcagt
      481 tacttgggaa gagcaagcgt actccaattg cacaggccct ggcttcagcc tccactcaga
      541 tattgtcatg ccctatattg cgaattacgg cacaaaagaa cagatcgaga agttcatccc
      601 ccagatgacg gcaggcaagt gtatcggtgc catagccatg acagagcctg ggggccggaa
      661 gtgacttac
//
