LOCUS       sequence-30228-e         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Hirschey MD, Shimazu T, Goetzman E, Jing E, Schwer B, Lombard DB, 
            Grueter CA, Harris C, Biddinger S, Ilkayeva OR, Stevens RD, Li Y, 
            Saha AK, Ruderman NB, Bain JR, Newgard CB, Farese RV, Alt FW, Kahn 
            CR, Verdin E
  TITLE     SIRT3 regulates mitochondrial fatty-acid oxidation by reversible 
            enzyme deacetylation.
  JOURNAL   Nature. 2010 Mar 4. 464(7285):121-5.
  PUBMED    20203611
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Mar 4. 464(7285):121-5."
COMMENT     Sequence Label: pTrcHisB mLCAD (29-430 aa)
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..28
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             32..64
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(68..76,77..91)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ggggggttct catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat
       61 gggtcgggat ctgtacgacg atgacgataa ggatccgcat tctggagcag aagcgcgtct
      121 agaaactcct tctgctaaaa aattaactga cattggaatc aggagaatct tttcctcgga
      181 gcatgacatt ttccgggaga gtgtaaggaa gtttttccaa gaagaagtga ttcctcacca
      241 cacagaatgg gagaaagctg gagaagtgag tagagaggtc tgggaaaaag ctggcaagca
      301 gggcttgctt ggcatcaaca tcgcagagaa acatggcggc attggtgggg acttgctctc
      361 aacagcagtt acttgggaag agcaagcgta ctccaattgc acaggccctg gcttcagcct
      421 ccactcagat attgtcatgc cctatattgc gaattacggc acaaaagaac agatcgagaa
      481 gttcatcccc cagatgacgg cgggcaagtg tatcggtgcc atagccatga cagagcctgg
      541 ggctggaagt gacttacaag gagtaagaac gaacgccaaa agatctggga gtgattggat
      601 tctcaatgga agcaaggtgt tcatcactaa tggctggtta agtgatctcg tgat
//
