LOCUS       sequence-30254-e         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Coward P, Wada HG, Falk MS, Chan SD, Meng F, Akil H, Conklin BR.
  TITLE     Controlling signaling with a specifically designed Gi-coupled 
            receptor.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Jan 6;95(1):352-7.
  PUBMED    9419379
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1998-01-6"; volume: "95(1)"; pages: "352-7"
COMMENT     Sequence Label: pcDNA3-Ro2
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           113..155
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             166..255
                     /codon_start=1
                     /product="signal sequence from bovine prolactin"
                     /label=prolactin signal sequence
                     /translation="MDSKGSSQKGSRLLLLLVVSNLLLCQGVVS"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgggccc cccctcgagc agctgaagct tgcttgttct
      121 ttttgcagaa gctcagaata aacgctcaac tttggcagat ccaccatgga cagcaaaggt
      181 tcgtcgcaga aagggtcccg cctgctcctg ctgctggtgg tgtcaaatct actcttgtgc
      241 cagggtgtgg tctccgatta caaagatgat gatgatgtcg actccccgat ccagatcttc
      301 cgcggggagc cgggccctac ctgcgccccg agcgcctgcc tgccccccaa cagcagcgcc
      361 tggtttcccg gctgggccga gcccgacagc aacggcagcg ccggctcgga ggacgcgcag
      421 ctggagcccg cgcacatctc cccggccatc ccggtcatca tcacggcggt ctactccgta
      481 gtgttcgtcg tgggcttggt gggcaactcg ctggtcatgt tcgtgatcat ccgatacaca
      541 aagatgaaga cagcaaccaa catttacata tttaacctgg ctttggcaga tgctttagtt
      601 actacaacca tgccctttca gagtacggtc tacttgatga attcttggcc ttttggagat
      661 gttctgtgca agattgtcat ttccattgac tactacaaca
//
