LOCUS       sequence-30256-e         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  TITLE     RO1
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-Ro1
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        34..52
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           100..142
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             153..242
                     /codon_start=1
                     /product="signal sequence from bovine prolactin"
                     /label=prolactin signal sequence
                     /translation="MDSKGSSQKGSRLLLLLVVSNLLLCQGVVS"
ORIGIN
        1 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
       61 agcttggtac cgggcccccc ctcgagcagc tgaagcttgc ttgttctttt tgcagaagct
      121 cagaataaac gctcaacttt ggcagatcca ccatggacag caaaggttcg tcgcagaaag
      181 ggtcccgcct gctcctgctg ctggtggtgt caaatctact cttgtgccag ggtgtggtct
      241 ccgattacaa agatgatgat gatgtcgact ccccgatcca gatcttccgc ggggagccgg
      301 gccctacctg cgccccgagc gcctgcctgc cccccaacag cagcgcctgg tttcccggct
      361 gggccgagcc cgacagcaac ggcagcgccg gctcggagga cgcgcagctg gagcccgcgc
      421 acatctcccc ggccatcccg gtcatcatca cggcggtcta ctccgtagtg ttcgtcgtgg
      481 gcttggtggg caactcgctg gtcatgttcg tgatcatccg atacacaaag atgaagacag
      541 caaccaacat ttacatattt aacctggctt tggcagatgc tttagttact acaaccatgc
      601 cctttcagag tacggtctac ttgatgaatt cttggccttt tggagatgtt ctgtgcaaga
      661 ttgtcatttc cattgactac tacaa
//
