LOCUS       sequence-30266-e         541 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Singla V, Hunkapiller J, Santos N, Seol AD, Norman AR, Wakenight P, 
            Skarnes WC, Reiter JF
  TITLE     Floxin, a resource for genetically engineering mouse ESCs.
  JOURNAL   Nat Methods. 2010 Jan . 7(1):50-2.
  PUBMED    19966808
REFERENCE   2  (bases 1 to 541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2010 Jan . 7(1):50-2."
COMMENT     Sequence Label: pFloxin
FEATURES             Location/Qualifiers
     source          1..541
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(11..58)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    76..210
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(100..119)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     154..173
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tcgcgtcgac gaagttccta tactttctag agaataggaa cttcggaata ggaacttctc
       61 gacgcggtac ccccggatct agacatgata agatacattg atgagtttgg acaaaccaca
      121 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
      181 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
      241 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta cagatgtgat
      301 atggctgatt atgatcatta cttatctagc cccccctcga ggtcgctcta gagcgacccc
      361 actgggctgt aggtggtgga tccgctagcg atatcactag tggcgcgccg catgcaccgg
      421 tacctggttg tcatggagga gaaagggcag aggttactgg ttgctggagt ctagctactt
      481 atccacaacc aacgcaccca agctagcttg aggttgcaga tactgggggt gggggggggg
      541 g
//
