LOCUS       sequence-30279-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human JMJD2B with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Cloos PA, Christensen J, Agger K, Maiolica A, Rappsilber J, Antal T,
            Hansen KH, Helin K
  TITLE     The putative oncogene GASC1 demethylates tri- and dimethylated 
            lysine 9 on histone H3.
  JOURNAL   Nature. 2006 Jul 20. 442(7100):307-11.
  PUBMED    16732293
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2006 Jul 20. 442(7100):307-11."
COMMENT     Sequence Label: pCMV-HA-JMJD2B
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             153..179
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    192..216
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagaggataa atactctgag tccaaccggg cccctctgct aaccatgttc atgccttctt
       61 ctctttccta cagctcctgg gcaacgtgct ggttgttgtg ctgtctcatc attttggcaa
      121 agaattcctc gacggatcaa ttcaccatgg catacccata cgatgttcca gattacgcta
      181 gcggatcatc aacaagtttg tacaaaaaag caggctcttt aaaggaacca attcagtcga
      241 ctggatccgg taccgccatg gggtctgagg accacggcgc ccagaacccc agctgtaaaa
      301 tcatgacgtt tcgcccaacc atggaagaat ttaaagactt caacaaatac gtggcctaca
      361 tagagtcgca gggagcccac cgggcgggcc tggccaagat catccccccg aaggagtgga
      421 agccgcggca gacgtatgat gacatcgacg acgtggtgat cccggcgccc atccagcagg
      481 tggtgacggg ccagtcgggc ctcttcacgc agtacaatat ccagaagaag gccatgacag
      541 tgggcgagta ccgccgcctg gccaacagcg agaagtactg taccccgcgg caccaggact
      601 ttgacgacct tgaacgcaaa tactggaaga acctcacctt tgtctccccg atctacgggg
      661 ctgacatcag cggctctttg tatgatgacg acgtggccca gtggaacatc gggagcctcc
      721 ggaccatcct ggacatggtg gagcgcgagt gcggcaccat catcgagggc gtgaacacgc
      781 cctacctgta cttcggcatg tggaagacca ccttcgcctg gcacaccgag aacatggacc
      841 tgtacagcat ca
//
