LOCUS       sequence-302864-        1449 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-LRRC4C-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..1449
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             87..104
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
ORIGIN
        1 caaacatgtc ctagcgtgtg cagctgtagc aaccagttca gcaaagtgat ctgcgtgcgg
       61 aagaacctga gagaggtgcc agacggcatc agcaccaaca ccagactgct gaacctgcac
      121 gagaaccaga tccagatcat caaagtgaac agcttcaagc acctgaggca cctggaaatc
      181 ctgcagctga gccggaacca catcagaaca atcgagatcg gcgccttcaa cggcctggcc
      241 aacctgaata ccctggaact gttcgacaac cggctgacca ccattcctaa tggcgccttc
      301 gtgtacctga gcaagctgaa agagctgtgg ctgcggaaca accccatcga gtctatcccc
      361 agctacgcct tcaatagaat ccccagcctg agaaggctgg acctgggaga gctgaagaga
      421 ctgagctata tcagcgaggg cgcctttgag ggcctgagca acctcagata cctgaacctg
      481 gccatgtgca atctgagaga gatccccaat ctgacccctc tgatcaagct ggacgagctg
      541 gatctgagcg gcaaccacct gtctgccatc agacctggaa gtttccaggg cctgatgcat
      601 ctgcagaaac tgtggatgat ccagagccag atccaagtga tcgagcggaa cgccttcgac
      661 aatctgcaga gcctggtgga aatcaatctg gcccacaaca acctgacact gctgccccac
      721 gatctgttca cacctctgca ccacctggaa cgcatccatc tgcatcacaa cccctggaat
      781 tgcaactgcg acatcctgtg gctgagctgg tggatcaagg acatggcccc tagcaatacc
      841 gcctgctgcg ccagatgcaa cacccctcct aacctgaagg gcagatatat cggcgagctg
      901 gaccagaact acttcacctg ttacgcccct gtgatcgtgg aacctcctgc cgacctgaat
      961 gtgacagaag gcatggccgc cgagctgaag tgtagagcct ctaccagcct gaccagcgtg
     1021 tcctggatca cccctaatgg cacagtgatg acccacggcg cctacaaagt gcggattgcc
     1081 gtgctgtccg acggcaccct gaatttcaca aacgtgaccg tgcaggacac cggcatgtac
     1141 acctgtatgg tgtccaacag cgtgggcaat accacagcca gcgccacact gaacgtgaca
     1201 gccgccacaa ccacaccttt cagctacttc agcaccgtga cagtggaaac catggaaccc
     1261 agccaggatg aggccagaac caccgataac aacgtgggcc ctacacctgt ggtggactgg
     1321 gaaaccacca acgtgaccac aagcctgaca cctcagagca ccagaagcac cgagaaaacc
     1381 ttcacaatcc ccgtgaccga catcaacagc ggcatccctg gcatcgacga agtgatgaag
     1441 accaccaaa
//
