LOCUS       sequence-302962-        1818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-DNER-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..1818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1671..1680
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aatcctgttc ctgctgctcc tctttctgcc cctggacctt gtgctgccca gccttgtaga
       61 aatggcggcg tgtgtaccag cagacccgag cctgatcctc agcatcctgc tccagctggc
      121 gagcctggct actcttgtac atgtcctgcc ggaatcagcg gcgccaattg tcagctggtg
      181 gccgatcctt gtgccagcaa tccttgccac cacggcaatt gcagcagcag ctctagcagc
      241 tcctccgacg gctacctgtg catctgcaat gagggctacg agggccccaa ttgcgaacag
      301 gctctgcctt ctctgcctgc cactggctgg acagaatcta tggcccctag acagctgcag
      361 cccgtgccag ctacacaaga gcccgataag atcctgcctc ggagccaggc tacagtgacc
      421 ctgcctacat ggcagcctaa gaccggccag aaagtggtgg aaatgaagtg ggaccaagtg
      481 gaagtgatcc ccgatatcgc ctgcggcaac gccagcagca attcttctgc tggcggcaga
      541 ctggtgtcct tcgaggtgcc acagaacacc agcgtgaaga tcagacagga tgccaccgcc
      601 agcctgattc tgctgtggaa agtgaccgcc accggcttcc agcagtgctc tctgatcgat
      661 ggcagaagcg tgacacccct gcaagcaagc ggaggactgg tgctgctcga agaaatgctg
      721 gccctgggca acaaccactt catcggcttc gtgaacgaca gcgtgaccaa gagcatcgtg
      781 gccctgagac tgaccctggt ggtcaaggtg tccacatgtg tgcctggcga gagccacgcc
      841 aacgatctgg aatgttctgg caagggcaag tgcaccacca agcctagcga agccaccttc
      901 agctgtacct gcgaggaaca gtacgtgggc accttctgcg aagagtacga cgcctgccag
      961 agaaagccct gtcagaacaa cgcctcctgc atcgacgcca atgagaagca ggacggcagc
     1021 aacttcacct gtgtgtgtct gcctggctat accggcgagc tgtgccagag caagatcgac
     1081 tactgcattc tggacccctg cagaaacggc gccacctgta tctctagcct gagcggcttt
     1141 acctgtcagt gccccgaggg ctattttggc agcgcctgtg aagagaaggt ggacccttgt
     1201 gcaagctccc catgccaaaa caacggcacc tgttacgtgg acggcgtgca ctttacctgc
     1261 aactgcagcc caggcttcac tggccctaca tgtgcccagc tgatcgactt ctgtgctctg
     1321 agcccttgcg ctcacggcac atgcagatct gtgggcacca gctacaagtg cctgtgcgat
     1381 cctggctatc acggcctgta ttgcgaagag gagtacaacg agtgcctgag cgccccttgt
     1441 ctgaatgccg ccacttgcag agatctggtc aacggctacg agtgcgtgtg tctggccgag
     1501 tacaagggca cccactgcga gctgtacaag gacccatgtg ccaacgtgtc ctgcctgaac
     1561 ggcgctacat gtgatagcga cggcctgaat ggaacctgca tctgcgcccc tgggtttacc
     1621 ggcgaagagt gcgatatcga catcaatgag tgcgacagca acccctgtca ccatggcggc
     1681 tcttgtctgg accagcctaa cggctacaac tgccactgtc ctcacggatg ggtcggagcc
     1741 aactgcgaga ttcatctgca gtggaagtcc ggccacatgg ccgagagcct gaccaacatg
     1801 cctcggcaca gtctgtat
//
