LOCUS       sequence-303048-        2247 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-VSIG10L-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..2247
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1646..1657)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ttgcagcaga ccaatttttc cagcgccttc agcagcgaca gcaagagcag ctctcaaggc
       61 ctgggcgtcg aggtgccatc tatcaagcct ccaagctgga aggtgcccga ccagtttctg
      121 gattctaagg ccagcgccgg catcagcgat agctcttggt ttcctgaggc tctgagcagc
      181 aacatgagcg gcagcttttg gagcaatgtg tccgccgaag gccaggatct gagccctgtg
      241 tctcctttta gcgaaacccc tggcagcgag gtgttccccg atattagtga tcctcaggtg
      301 cccgctaagg accccaagcc tagctttaca gtgaaaaccc cagccagcaa catcagcacc
      361 caggtgtccc acacaaagct gagcgtggaa gcccctgaca gcaagttcag ccccgacgac
      421 atggatctga agctgtctgc ccagtctcca gagagcaagt tctctgccga aacacacagc
      481 gccgccagct ttccacaaca agttggagga cctctggccg tgctcgtggg cacaacaatt
      541 agactgcccc tggtgcctat tcctaatcct ggacctccta ccagcctggt tgtgtggcgg
      601 agaggatcta aagttctggc cgctggtgga cttggacctg gcgctcctct gatttctctg
      661 gaccccgctc acagggacca cctgagattt gatcaggcca gaggcgtgct ggaactggcc
      721 tctgctcaac tggatgatgc tggcgtgtac accgccgaag tgattagagc tggcgtcagc
      781 cagcagaccc acgagtttac agtgggagtg tacgagcctc tgcctcagct gtctgtgcag
      841 cctaaggctc ctgagacaga agaaggcgcc gctgagctga gactgagatg tcttggatgg
      901 ggacctggca gaggcgagct gtcttggagt agagatggca gagccctgga agccgctgaa
      961 tctgaagggg ccgagacacc cagaatgaga tctgaaggcg accagctgct gattgtgcgg
     1021 cctgttagaa gcgaccacgc cagatacaca tgcagagtgc ggagcccatt cggccataga
     1081 gaagctgctg ccgacgtgtc cgtgttctat ggccctgatc ctccaaccat caccgtgtcc
     1141 tctgacagag atgccgctcc tgccagattt gtgacagccg gcagcaatgt gaccctgaga
     1201 tgtgctgctg cttccagacc tcctgccgac attacatggt cactggccga tcctgctgaa
     1261 gctgcagttc ctgctggatc taggctgctg ttgcctgccg ttggaccagg acatgccgga
     1321 acatatgcct gtctggccgc caatcctaga accggcagaa gaagaagatc cctgctgaac
     1381 ctgaccgtgg ccgatcttcc accaggtgct cctcagtgtt ctgttgaagg cggccctggc
     1441 gacagatccc tgagattcag atgttcttgg cctggcggag caccagctgc aagtctgcaa
     1501 tttcaaggac tgcccgaggg catcagagcc ggacctgttt cttctgttct gctggccgca
     1561 gtgcctgctc atcctagact gagcggagtg cctatcacat gcctggccag acatctggtg
     1621 gccacaagga cctgtacagt gacccctgaa gctcccagag aggttctgct gcatccactg
     1681 gtggccgaaa caagactggg agaagccgaa gtggctctgg aagcctctgg atgtcctcca
     1741 cctagtagag cctcttgggc cagagaaggc agacctcttg caccaggcgg aggaagtaga
     1801 ctgagactca gccaggatgg cagaaagctg cacatcggca acttcagcct ggactgggat
     1861 ctgggcaatt acagcgtgct gtgttctggc gctcttggag ccggcggaga tcagatcaca
     1921 ctgatcggcc cttctatcag ctcctggcga ctgcagaggg ctagagatgc agctgtgctg
     1981 acctgggatg ttgagagagg cgccctgatc tctagctttg agatccaggc ttggcctgat
     2041 ggcccagctc tgggcagaac cagcacatac agagattggg tgtcactgct gatcctgggg
     2101 cctcaagaaa gatctgctgt ggtgcccctg cctcctagaa atcctggcac ctggaccttc
     2161 agaatcctgc ctattctcgg cggccagcct ggcacaccat ctcagagtag agtgtacaga
     2221 gctggcccta cactgagcca tggtgct
//
