LOCUS       sequence-303078-        2454 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-LEPR-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..2454
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tttaatctga gctaccccat cacaccctgg cggttcaagc tgagctgcat gcctcctaac
       61 tctacctacg actacttcct gctgcctgcc ggcctgagca agaacaccag caatagcaac
      121 ggccactacg agacagccgt ggaacccaag ttcaacagca gcggcaccca cttcagcaac
      181 ctgtccaaga ccaccttcca ctgctgcttc agatccgagc aggaccggaa ttgcagcctg
      241 tgcgccgata acatcgaggg caagaccttc gtgtccaccg tgaacagcct ggtgttccag
      301 cagatcgacg ccaactggaa tatccagtgc tggctgaagg gcgacctgaa gctgttcatc
      361 tgctacgtgg aaagcctgtt caagaacctc ttccggaact acaactacaa ggtccacctc
      421 ctgtacgtgc tgcccgaggt gctggaagat tctccactgg tgcctcagaa aggcagcttc
      481 cagatggtgc actgcaactg cagcgtgcac gagtgctgcg aatgtctggt gcctgtgcct
      541 accgccaagc tgaacgatac cctgctgatg tgcctgaaga tcacctctgg cggcgtgatc
      601 tttcagagcc ctctgatgag cgtgcagccc atcaacatgg tcaagcccga tcctccactg
      661 ggactgcaca tggaaatcac cgacgacggc aatctgaaga tctcttggag cagccctcct
      721 ctggtgccct ttccactgca ataccaagtg aagtacagcg agaacagcac caccgtgatc
      781 cgcgaggccg ataagatcgt gtctgccaca agcctgctgg tggactctat cctgcctggc
      841 agcagctacg aggtgcaagt gcggggcaaa agactggacg gccctggcat ttggagcgat
      901 tggagcaccc ctagagtgtt cacgacccag gacgtgatct acttcccacc taagatcctg
      961 accagcgtgg gcagcaacgt gtcctttcac tgcatctaca agaaagagaa caagatcgtc
     1021 cccagcaaag aaatcgtgtg gtggatgaac ctggccgaga agatccctca gagccagtac
     1081 gacgtggtgt ccgaccacgt gtccaaagtg accttcttca acctgaacga gacaaagccc
     1141 agaggcaagt tcacctacga cgccgtgtac tgctgcaacg agcacgaatg ccaccacaga
     1201 tacgccgagc tgtacgtgat cgacgtgaac atcaacatca gctgcgagac agacggctac
     1261 ctgaccaaga tgacctgccg gtggtctacc agcaccatcc agtctctggc cgagtctacc
     1321 ctgcagctga gataccacag aagcagcctg tactgcagcg acatccccag cattcacccc
     1381 atcagcgagc ccaaggattg ctacctgcag agcgacggct tctacgagtg tatctttcag
     1441 cccatctttc tgctgagcgg ctacaccatg tggatcagaa tcaaccacag cctgggcagc
     1501 ctggactccc ctcctacatg tgtgctgcct gacagcgtcg tgaagcctct gcctccatct
     1561 tctgtgaagg ccgagatcac catcaatatc ggcctgctga agattagctg ggagaagcct
     1621 gtgttccccg agaacaacct gcagttccag atcagatacg gcctgtccgg caaagaggtc
     1681 cagtggaaga tgtacgaggt gtacgacgcc aagagcaaga gcgtgtcact gcccgtgcct
     1741 gatctgtgtg ccgtgtatgc agtgcaagtc cggtgcaagc ggctggatgg actcggctac
     1801 tggtccaact ggtctaaccc tgcctacacc gtggtcatgg acatcaaggt gcccatgaga
     1861 ggccctgagt tctggcggat catcaacggc gacaccatga agaaagaaaa gaacgtgacc
     1921 ctgctgtgga agcccctgat gaagaacgac tccctgtgct ccgtgcagag atacgtgatc
     1981 aaccaccaca ccagctgcaa tggaacttgg agcgaggacg tgggcaacca caccaagttt
     2041 acctttctgt ggaccgagca ggcccacacc gtgactgtgc tggccatcaa ttctatcggc
     2101 gccagcgtgg ccaacttcaa tctgaccttc agctggccca tgagcaaagt gaacatcgtg
     2161 cagagcctga gcgcctatcc tctgaacagc agctgcgtga tcgtgtcctg gattctgagc
     2221 cccagcgact acaagctgat gtacttcatc atcgagtgga agaacctgaa tgaggacggc
     2281 gagatcaagt ggctgcggat cagctccagc gtgaagaagt actacatcca cgaccacttc
     2341 atccccatcg agaagtacca gttctctctg taccccatct ttatggaagg cgtgggcaag
     2401 cccaagatca tcaatagctt cacccaggat gatattgaga agcaccagtc cgat
//
