LOCUS       sequence-303082-        2475 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-BOC-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..2475
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2444..2453
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gatctgaatg aggtgccaca agttacagtg cagcctgcca gcacagtgca gaaacctggc
       61 ggaacagtga tcctgggctg tgtggtggaa cctcctagaa tgaatgtgac ctggcggctg
      121 aacggcaaag agctgaacgg atctgacgat gccctgggcg tgctgatcac acacggcaca
      181 ctggttatca cagccctgaa caaccacacc gtgggcagat accagtgcgt ggcaagaatg
      241 cctgctggcg ctgttgcttc tgtgcctgct acagtgacac tggccaacct gcaggacttc
      301 aagctggacg tgcagcacgt gatcgaggtg gacgagggaa ataccgccgt gatcgcctgt
      361 catctgcccg agtctcaccc taaggctcaa gtgcggtaca gcgtgaagca agagtggctg
      421 gaagccagca gaggcaacta cctgatcatg cccagcggaa acctgcagat cgtgaacgcc
      481 tctcaagagg acgagggcat gtacaagtgc gccgcctaca atcccgtgac acaagaagtg
      541 aaaaccagcg gcagcagcga cagactgaga gtgcggagat ctacagccga ggccgccaga
      601 attatctacc ctcctgaggc tcagaccatc atcgtgacaa agggccagag cctgatcctg
      661 gaatgcgtgg cctctggaat ccctccacct agagtgacat gggccaaaga tggcagctcc
      721 gtgaccggct acaacaagac cagattcctg ctgagcaacc tgctgatcga caccaccagc
      781 gaggaagata gcggcaccta cagatgcatg gccgacaatg gtgttggaca gcctggcgcc
      841 gctgtgatcc tgtacaatgt gcaggttttc gagcctcctg aagtgaccat ggaactgagc
      901 cagctggtta tcccttgggg ccagtctgcc aagctgacat gtgaagtgcg gggcaatcct
      961 ccaccttccg tgctgtggct gagaaacgcc gtgcctctga tcagctccca gagactgaga
     1021 ctgtctcgga gagcactgag ggtgctgtct atgggacctg aggatgaagg cgtgtaccag
     1081 tgtatggccg agaatgaagt gggctctgcc catgctgtgg tgcagctgag aacaagcaga
     1141 cccagcatca cccctagact gtggcaggat gctgaactgg ctacaggcac acctcctgtg
     1201 tctcccagca agctgggaaa ccctgagcag atgctgagag gacagccagc tctgcctaga
     1261 cctcctacat ctgtgggacc tgcctctcct cagtgtcctg gcgaaaaagg acaaggcgct
     1321 cctgccgagg ctcccatcat tctgtctagc cctagaacca gcaagaccga cagctacgaa
     1381 ctcgtgtggc ggcctagaca cgaaggatct ggcagagcac ccatcctgta ctacgtggtc
     1441 aagcaccgga aagtgaccaa cagctccgac gattggacca tcagcggcat ccctgccaat
     1501 cagcacaggc tgacactgac aagactggac cctggcagcc tgtacgaagt ggaaatggcc
     1561 gcctataatt gtgccggcga gggccagacc gccatggtta cttttagaac cggcagacgg
     1621 cccaagcctg agatcatggc ctctaaagag cagcagatcc agagggacga tcctggcgcc
     1681 tctccacaat ctagctctca gccagatcac ggcaggctgt ctccaccaga agctcccgat
     1741 agacctacca tcagcaccgc cagcgagaca agcgtgtacg tgacctggat tcccagagga
     1801 aacggcggct tccccatcca gtccttccgg gtcgagtaca agaagctgaa gaaagtcggc
     1861 gactggattc tggccaccag cgccattcct ccaagcagac tgtctgtgga aatcaccggc
     1921 ctggaaaagg gcaccagcta caagtttaga gtgcgggccc tgaatatgct gggcgagtct
     1981 gaacctagcg ctcccagcag accttacgtg gtgtctggct atagcggcag agtgtacgaa
     2041 agacccgtgg ctggccctta catcaccttc accgatgccg tgaacgagac aaccatcatg
     2101 ctgaagtgga tgtatatccc cgccagcaac aacaacaccc ctatccacgg cttctacatc
     2161 tactacagac ccaccgacag cgacaacgac tccgactaca agaaagacat ggtggaaggc
     2221 gacaagtact ggcacagcat cagccatctg cagcccgaaa ccagctacga catcaagatg
     2281 cagtgcttca acgaaggcgg cgagagcgag ttcagcaacg tgatgatttg cgagacaaag
     2341 gcccggaagt ctagcggcca accaggcaga cttcctcctc ctacacttgc tcctccacag
     2401 cctcctctgc ctgagacaat cgaaagacct gttggcacag gcgccatggt ggctagaagc
     2461 agcgatctgc cttat
//
