LOCUS       sequence-303104-        2613 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-ROBO3-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..2613
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggcgatatca gcaattcttc tgaactgctg ctgggcttca acagcagcct ggccgctctg
       61 aatcacacct tgcttccacc tggcgacccc agcctgaatg gaagtagagt gggacccgag
      121 gacgccatgc ctagaatcgt tgagcagcct cctgacctgc tggtgtccag aggcgaacct
      181 gctacactgc cttgcagagc cgaaggcaga cccagaccta acatcgagtg gtacaagaac
      241 ggcgccagag tggccacagt gcgcgaagat cctagagcac ataggctgct gctgcctagc
      301 ggagccctgt tcttcccaag aatcgtgcat ggcagacggg ccagacctga cgaaggcgtg
      361 tacacttgcg tggccagaaa ctatctgggc gctgccgctt ccagaaacgc ctctctggaa
      421 gtggccgtgc tgcgggacga tttcagacag tctcctggca atgtggtggt ggccgtggga
      481 gaacctgctg tgctggaatg tgtgcctcct agaggacacc ctgagccttc cgtgtcttgg
      541 agaaaggatg gcgccagact gaaagaggaa gagggcagaa tcaccatcag aggcggcaag
      601 ctgatgatga gccacacact gaagtccgac gccgggatgt acgtgtgtgt ggcctctaat
      661 atggccggcg agagagaatc tgccgccgct gaagtgatgg tgctggaaag acccagcttc
      721 ctgagaaggc ccgtgaacca ggttgtgctg gctgatgccc ctgtgacctt cctgtgtgaa
      781 gtgaagggcg accctccacc tagactgaga tggcggaaag aggatggcga gctgcctacc
      841 ggcagatacg agatcagatc cgaccactct ctgtggatcg gccatgtgtc tgccgaggac
      901 gagggcacat atacctgtgt ggccgagaac tctgtgggga gagctgaggc ttctggcagc
      961 ctgtctgtgc atgtgcctcc acagctggtc acccagcctc aggatcaaat ggctgcccct
     1021 ggcgagtctg tggcctttca gtgtgaaaca aagggcaatc ctcctcctgc catcttttgg
     1081 cagaaagagg gctcccaggt cctgctgttc cctagccaat ctctgcagcc caccggccgc
     1141 ttttctgtgt caccaagagg ccagctgaac atcaccgctg tgcaaagagg cgacgccggc
     1201 tactatgtgt gtcaggctgt ttctgtggcc ggcagcattc tggccaaggc tctgctggaa
     1261 atcaagggcg cttccctgga tggactgcct ccagtgattc ttcagggccc agccaatcag
     1321 acactggtgc tgggaagtag cgtgtggctg ccctgtagag tgaccggcaa tccacagcct
     1381 agcgtgcggt ggaagaaaga cggacaatgg ctgcagggcg acgacctgca gtttaagacc
     1441 atggccaacg gcaccctgta tatcgccaac gtgcaagaga tggacatggg gttctactct
     1501 tgtgtggcca agagcagcac aggcgaggct acttggagcg gctggctgaa gatgagagag
     1561 gattggggcg tcagccccga tcctcctaca gagcctagta gtcctcctgg cgctccttct
     1621 cagcctgtgg tcaccgagat caccaagaac agcatcaccc tgacctggaa gcctaatcct
     1681 cagacaggcg ccgctgtgac ctcctacgtg atcgaggctt tttctcccgc cgctggcaat
     1741 acctggcgga cagttgctga tggcgtgcag ctggaaaccc acaccgtgtc tggactgcag
     1801 cctaacacca tctacctgtt cctcgtcaga gccgttggag cctggggact gagtgaaccc
     1861 tctcctgtgt ccgaacctgt gcggacccaa gacagctctc ctagcagacc cgtggaagat
     1921 ccttggagag gacagcaggg acttgccgag gtggccgtta gactgcaaga gccaattgtg
     1981 ctgggaccca gaacactcca ggtgtcctgg acagtggatg gccctgtgca actggtgcag
     2041 ggctttagag tctcttggag agtggctgga cctgaaggcg gctcttggac catgctggat
     2101 ctgcagtctc caagccagca gtccaccgtg cttagaggac tgccacctgg cacacagatc
     2161 cagatcaagg tgcaggccca gggccaagaa ggactgggag ctgaatctct gagcgtgacc
     2221 cgcagcatcc cagaggaagc tccatctgga cctccacagg gtgttgccgt tgcacttggc
     2281 ggagatggca acagctctat caccgtgtcc tgggaacctc cactgcctag ccagcagaat
     2341 ggcgtgatca ccgagtatca gatctggtgc ctgggcaacg agagccggtt ccacctgaat
     2401 agatctgccg ctggatgggc cagatccgcc atgctgagag gacttgttcc cggcctgctg
     2461 tacagaacac tggtggccgc tgctacatct gctggcgtgg gagttccttc tgctcctgtg
     2521 ctggttcagc tgccttctcc tcctgatctg gaacccggcc tggaagttgg agctggcctt
     2581 gctgttagac tggcccgggt tctgagagaa cct
//
