LOCUS       sequence-303140-        2931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-CNTN6-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..2931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2041..2055)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
ORIGIN
        1 gatggactgc tgagcagacc tatctttacc caagagcctc acgacgtgat cttcccactg
       61 gacctgagca agagcgaagt gatcctgaat tgcgccgcca acggctaccc atctccacac
      121 tacagatgga agcagaacgg caccgacatc gacttcacca tgagctacca ctacaggctg
      181 gatggcggca gcctggccat caatagccct cacacagacc aggacatcgg catgtaccag
      241 tgtctggcca ccaatctgct gggcaccatt ctgagcagaa aggccaagct gcagttcgcc
      301 tacatcgagg acttcgagac aaagaccaga tccaccgtgt ccgttagaga aggccaaggc
      361 gttgtgctgc tgtgtggccc tcctcctcat ttcggcgatc tgagctacgc ctggaccttc
      421 aacgacaacc ctctgtacgt gcaagaggac aaccgcagat tcgtgtccca agagacaggc
      481 aacctgtata tcgccaaggt ggaacccagc gacgtgggca actacacctg tttcatcacc
      541 aacaaagagg cccagcggag cgttcaggga cctcctacac ctctggttca gagaacagat
      601 ggcgtgatgg gcgagtacga gcccaagatc gaagtgcggt tccctgagac aatccaggcc
      661 gccaaggata gcagcgtgaa gctggaatgc ttcgccctgg gcaaccctgt gcctgacatt
      721 tcttggagaa ggctggacgg aagccctctg cctggcaaag tgaagtacag caagagccag
      781 gccatcctgg aaatccccaa cttccagcaa gaggatgagg gcttctacga gtgtatcgcc
      841 agcaacctga gaggcagaaa tctggccaag ggccagctga tcttttacgc ccctcctgag
      901 tgggagcaga agatccagaa tacccacctg agcatctacg acaacctgct gtgggagtgc
      961 aaggccagcg gaaagcctaa tccttggtac acctggctga agaacggcga gagactgaac
     1021 cccgaggaaa gaatccagat cgagaatggc accctgatca tcaccatgct gaacgtgtcc
     1081 gacagcggag tgtatcagtg tgccgccgag aacaagtacc agatcatcta cgccaacgcc
     1141 gagctgagag tgctggcctc cgctcctgat ttcagcaagt cccctgtgaa gaaaaagtcc
     1201 ttcgtgcaag tcggcggcga catcgtgatc ggctgcaagc caaatgcctt tcctagagcc
     1261 gccatcagct ggaagagagg cacagagaca ctgcggcaga gcaagagaat ctttctgctg
     1321 gaagatggca gcctgaagat ctacaacatc accagaagcg acgccggcag ctacacatgc
     1381 attgccacaa atcagttcgg caccgccaag aacaccggaa gcctgatcgt gaaagaacgg
     1441 accgtgatca ccgtgcctcc aagcaagatg gatgtgaccg tgggcgagag catcgtgctg
     1501 ccttgtcaag tgtcccacga tcctagcatc gaggtggtgt tcgtgtggtt cttcaacggg
     1561 gacgtgatcg acctgaagaa aggcgtggcc cactttgaga gaatcggcgg agaatctgtg
     1621 ggcgacctga tgatccggaa catccagctg caccacagcg gcaagtacct gtgtaccgtg
     1681 cagaccacac tggaatctct gagcgccgtg gccgatatca tcgttagagg acctccagga
     1741 ccacctgagg acgtgcaggt cgaggatatc agcagcacca caagccagct gtcttggaga
     1801 gccggacctg acaacaacag ccccatccag atcttcacaa tccagaccag aacacccttc
     1861 agcgttggat ggcaggctgt ggccactgtg ccagagattc tgaacggcaa gacctacaac
     1921 gccaccgtcg ttggactgag cccttgggtc gagtatgagt ttagagtggt ggccggcaac
     1981 agcatcggaa tcggagagcc ttctgagcct agcgagctgc tgagaacaaa agcctctgtg
     2041 ccagtggtgg ctcccgtgaa tatccatggt ggtggcggaa gcagatccga gctggtcatc
     2101 acatgggaga gcatccctga ggaactgcag aatggcgaag gcttcggcta catcatcatg
     2161 ttcagacccg tgggctccac cacctggtcc aaagaaaagg tgtccagcgt ggaaagcagc
     2221 cgcttcgtgt accggaatga gagcatcatc cctctgagcc ccttcgaagt gaaagtgggc
     2281 gtgtacaaca acgaaggcga gggcagcctg tctaccgtga caatcgtgta ctctggcgag
     2341 gatgagcccc agctggctcc tagaggaaca agcctgcaga gctttagcgc cagcgagatg
     2401 gaagtgtcct ggaacgccat tgcctggaac agaaacacag gccgcgtgct gggctacgag
     2461 gtgctgtact ggaccgacga cagcaaagaa tctatgatcg gcaagatcag agtgtccggc
     2521 aacgtgacca ccaagaacat caccggcctg aaggccaaca ccatctactt tgcctccgtg
     2581 cgggcctaca ataccgccgg aacaggacct agcagccctc ctgtgaatgt gaccacaaaa
     2641 aagagccctc caagccagcc tcctgccaat atcgcctgga agctgaccaa cagcaagctg
     2701 tgtctgaact gggagcacgt caagaccatg gaaaacgaga gcgaggtcct gggctacaag
     2761 atcctgtacc ggcagaaccg gcagtctaag acccacattc tggaaacaaa caacaccagc
     2821 gccgaactgc tggtgccctt cgaggaagat tacctgatcg agatccggac cgtgtctgac
     2881 ggcggagatg gatctagcag cgaggaaatc agaatcccca agatgtcctc t
//
