LOCUS       sequence-30315-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human E2F5 with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  TITLE     K Helin plasmid
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMVHA-E2F5
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             161..187
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      195..204
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             358..375
                     /codon_start=1
                     /product="recognition and cleavage site for Genenase(TM) I,
                     a subtilisin BPN' variant engineered for increased 
                     substrate specificity (Carter and Wells, 1987)"
                     /label=Genenase(TM) I site
                     /translation="PGAAHY"
ORIGIN
        1 tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat
       61 gccttcttct ctttcctaca gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat
      121 tttggcaaag aattcctcga cggatcaatt caccatggca tacccatacg atgttccaga
      181 ttacgctagc ggatccatgg cggcggcaga gcccgcgagc tcgggccagc aggcgccggc
      241 agggcagggg cagggccagc ggccgccgcc gcagcctccg caggcgcaag ccccgcagcc
      301 gcccccgccg ccgcagctcg ggggcgcggg gggcggcagc agcaggcacg agaagagcct
      361 ggggctgctc actaccaagt tcgtgtcgct gctgcaggag gccaaggacg gcgttctgga
      421 tctcaaagcg gctgctgata ctttggctgt gaggcaaaaa aggagaattt atgatatcac
      481 caatgtctta gagggaattg acttgattga aaaaaagtca aaaaacagta tccagtggaa
      541 aggtgtaggt gctggctgta atactaaaga agtcatagat agattaagat atcttaaagc
      601 tgaaattgaa gatctagaac tgaaggaaag agaacttgat cagcagaagt tgtggctaca
      661 gcaaagcatc aaaaatgtga tggacgattc cattaataat agattttcct atgtaactca
      721 tgaagacatc tgtaattgct ttaatggtga tacacttttg gccattcagg caccttctgg
      781 tacacaactg gaggtaccca ttccagaaat ggg
//
