LOCUS       sequence-303152-        3000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-SEMA5B-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..3000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttgtctcttg ttagaggcct gcttccttgc ctgccaccag gcgctagaac agccgaggga
       61 cctattatgg tgctggctgg acctctggcc gtgtctctgc ttctgccttc tctgaccctg
      121 ctggtgtctc acctgtctag cagccaggat gtgtccagcg agcctagctc tgagcagcag
      181 ctgtgtgccc tgagcaagca ccctacagtg gccttcgaag atctgcagcc ctgggtgtcc
      241 aacttcacat accctggggc cagagacttc tcccagctgg ctcttgatcc cagcggcaac
      301 cagctgattg tgggcgccag aaactacctg ttcagactga gcctggccaa cgtgtcactg
      361 ctgcaggcta cagagtgggc cagcagcgag gataccagaa gaagctgtca gagcaagggc
      421 aagaccgagg aagagtgcca gaactacgtg cgggtgctga tcgtggccgg cagaaaggtt
      481 ttcatgtgcg gcaccaacgc tttcagcccc atgtgcacca gcagacaagt gggcaacctg
      541 agccggacca tcgagaagat caacggcgtg gccagatgtc cctacgatcc cagacacaat
      601 agcaccgccg tgatcagctc tcagggcgaa ctgtatgccg ccaccgtgat cgatttcagc
      661 ggcagagatc ccgccatcta cagaagcctc ggatctggcc ctccactgag aacagcccag
      721 tacaacagca agtggctgaa cgagcccaac ttcgtggccg cctatgacat cggcctgttc
      781 gcctacttct tcctgcggga aaacgccgtg gaacacgact gtggcagaac cgtgtatagc
      841 agagtggcca gagtgtgcaa gaacgacgtc ggcggcagat tcctgctgga agatacctgg
      901 accaccttta tgaaggcccg gctgaactgc agcagacctg gcgaggtgcc cttctactac
      961 aacgagctgc agagcgcctt ccatctgcct gagcaggatc tgatctacgg cgtgttcacc
     1021 accaacgtga actctatcgc cgcctctgcc gtgtgcgcct ttaatctgtc tgccatcagc
     1081 caggccttta acggcccctt cagataccaa gagaacccca gagctgcctg gctgcctatc
     1141 gccaatccta ttcctaactt ccagtgcggc acactgcccg agacaggccc caatgagaat
     1201 ctgaccgaga gaagcctgca ggacgcccag agactgttcc tgatgtctga agccgtgcag
     1261 cccgtgacac ctgagccttg tgtgacacag gatagcgtgc ggttctccca cctggtggtg
     1321 gatctggtgc aggccaaaga caccctgtac cacgtgctgt acatcggcac agagagcggc
     1381 acaatcctga aggccctgtc taccgccagc agatccctgc acggatgcta cctggaagaa
     1441 ctgcacgtgc tgccacctgg cagaagagag cctctgagat ccctgagaat cctgcacagc
     1501 gccagggctc tgtttgtggg actgagagat ggcgtgctga gagtgcccct ggaaagatgt
     1561 gccgcctaca gatcacaggg cgcctgtctg ggagctagag atccttactg tggctgggac
     1621 ggcaagcagc agagatgtag caccctggaa gattccagca acatgagcct gtggacccag
     1681 aacatcaccg cctgtcctgt gcggaacgtg acacgagatg gcggctttgg accttggagt
     1741 ccttggcagc cttgcgagca tctggacggc gataatagcg gctcctgcct gtgtagagcc
     1801 agaagctgcg atagccccag acctagatgt ggcggcctgg attgtctggg ccctgccatc
     1861 catatcgcca actgctccag aaacggcgcc tggacacctt ggtcatcttg ggccctgtgt
     1921 agcacctctt gcggcatcgg atttcaagtc cggcagcgga gctgcagcaa ccctgctcct
     1981 agacatggcg gcagaatctg cgtgggcaag tcccgggaag agagattctg caacgagaac
     2041 accccttgtc cagtgcctat cttctgggcc tcttggggca gctggtccaa gtgcagctct
     2101 aattgtggcg gcggaatgca gagcagaaga agggcctgcg agaacggcaa tagctgtctc
     2161 ggatgcggcg tggaattcaa gacctgcaat cctgagggct gccccgaagt gcggagaaac
     2221 acaccttgga ctccatggct gcccgtgaac gttacacaag gcggagccag acaagagcag
     2281 cggttccggt ttacctgtag agcccctctg gctgatcctc acggcctgca gttcggaaga
     2341 agaagaaccg agacacggac ctgtcctgcc gatggcagcg gctcttgtga tacagatgcc
     2401 ctggtggaag tgctgctgag aagcggctct acaagccctc acacagtgtc tggtggatgg
     2461 gctgcttggg gaccttggtc cagctgtagc agagattgcg agctgggctt cagagtgcgg
     2521 aagcggacct gtaccaatcc agagcctagg aacggcggcc tgccatgtgt tggagatgcc
     2581 gccgaatacc aggactgcaa ccctcaggcc tgtccagtta gaggcgcctg gtcttgttgg
     2641 acctcttgga gcccttgttc cgcctcttgt ggcggaggcc actaccagag aacaagaagc
     2701 tgcacaagcc ccgcaccttc tccaggcgag gatatttgcc tgggcctgca tactgaagag
     2761 gccctgtgcg caacacaggc ttgtccagaa ggatggtccc cttggagcga gtggtctaag
     2821 tgcacagatg acggcgccca gagcagatct aggcactgcg aggaactgct gcctggatct
     2881 tctgcctgtg ccggcaattc tagccagagc cggccttgtc catacagcga gatcccagtg
     2941 atcctgcctg ccagctctat ggaagaggcc acagattgcg ccggcttcaa cctgatccat
//
