LOCUS       sequence-303169-        9322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9322)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-NEO1-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..9322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             6664..6708
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             6709..6726
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6866..6883)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6872..6983
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7101..7121)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7106..7435
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7286..7421
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7348..7367
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7483..7530
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7549..8148
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7549..7568)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8045..8065
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8278..8399
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8315..8334)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8369..8388
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8452..9243,9244..9312))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9075..9094
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9313..9322,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccgc aagatctgga
     2761 tctgctcctc aatctcctgg cgcctccatc cggaccttca cacccttcta ctttctggtg
     2821 gaacccgtgg atacactgag cgtgcgggga agctctgtga tcctgaattg cagcgcctac
     2881 agcgagccct ctccaaagat cgagtggaag aaggacggca ccttcctgaa cctggtgtcc
     2941 gacgatagaa ggcagctgct gccagatggc agcctgttca tcagcaacgt ggtgcacagc
     3001 aagcacaaca agcccgacga gggctactat cagtgtgtgg ccactgtgga aagcctgggc
     3061 accatcatca gcagaaccgc caaactgatt gtggccggac tgcccagatt cacctctcag
     3121 cctgagcctt cttctgtgta cgccggcaac aacgccatcc tgaactgcga agtgaacgcc
     3181 gacctggtgc ctttcgtcag atgggagcag aacagacagc ccctgctgct ggacgacaga
     3241 gtgattaagc tgcctagcgg catgctggtc atctccaatg ctacagaagg cgacggcggc
     3301 ctgtacagat gtgtggttga atctggcggc cctcctaagt acagcgacga ggtggaactg
     3361 aaggtgctgc ccgatcctga agtgatcagc gatctggtgt tcctgaagca gccatctcca
     3421 ctcgtcagag tgatcggcca ggatgtggtg ctgccttgtg ttgcctctgg cctgcctaca
     3481 cctaccatca agtggatgaa gaacgaggaa gccctggaca ccgagagcag cgaaagactg
     3541 gttctgcttg ccggcggaag cctggaaatc tccgatgtga cagaggacga cgccggcacc
     3601 tatttctgta tcgccgacaa cggcaacgag acaattgagg cccaggccga actgacagtg
     3661 caggcccagc ctgagtttct gaaacagccc accaacatct acgcccacga gagcatggac
     3721 atcgtgttcg agtgtgaagt gaccggcaag cccacaccta ccgtgaaatg ggtcaagaac
     3781 ggcgacatgg tcatccccag cgactacttc aagatcgtga aagagcacaa tctgcaggtc
     3841 ctgggactcg tgaagtccga tgagggcttt taccagtgca ttgccgagaa cgacgtgggc
     3901 aatgctcaag ccggcgctca gctgatcatt ctggaacatg cccctgccac aacaggccct
     3961 ttgccttctg ctcctagaga tgtggtggcc agcctggtgt ctacccggtt tatcaagctg
     4021 acatggcgga cccctgccag cgatcctcat ggcgataatc tgacctacag cgtgttctac
     4081 accaaagagg gaatcgcccg cgagagagtg gaaaacacat ctcaccctgg cgagatgcaa
     4141 gtgaccatcc agaatctgat gcccgccacc gtgtacatct ttagagtgat ggcccagaac
     4201 aagcacggca gcggagaatc tagcgcccct ctgagagttg agacacagcc tgaagttcag
     4261 ctgcccggac ctgctccaaa tctgagagcc tatgccgcct ctcctaccag catcaccgtt
     4321 acctgggaga cacctgtgtc cggcaacggc gagatccaga actacaagct gtactacatg
     4381 gaaaagggca ccgacaaaga acaggacgtg gacgtgtcca gccacagcta caccatcaac
     4441 ggcctgaaga agtacaccga gtatagcttc agagtggtcg cctacaacaa acacggccct
     4501 ggcgttagca cacctgatgt ggccgtcaga accctgtctg atgtgccatc tgccgctcct
     4561 cagaacctga gcctggaagt gcggaacagc aagagcatca tgatccactg gcagcctcca
     4621 gcaccagcca cacagaatgg ccagatcacc ggctacaaga tccggtacag aaaggccagc
     4681 cggaagtccg acgtgaccga aacactggtg tctggcacac agctgagcca gctgatcgaa
     4741 ggcctggata gaggcaccga gtacaatttc agagtcgccg ctctgaccat caatggcaca
     4801 ggccctgcta cagattggct gtccgccgag acattcgaga gcgacctgga tgagacaaga
     4861 gtgcctgagg tgccaagctc tctgcatgtg cggcctctcg tgacctctat cgtggtgtct
     4921 tggacccctc ctgagaacca gaatatcgtc gtgcggggct atgccatcgg ctacggaatt
     4981 ggatctcccc acgcacagac aatcaaggtg gactacaagc agcggtacta cacaatcgag
     5041 aatctggacc ccagcagcca ctacgtgatc accctgaagg cctttaacaa cgtcggcgag
     5101 ggcatccctc tgtacgagtc tgctgtgacc agacctcaca ccgatacaag cgaggtggac
     5161 ctgttcgtga tcaacgcccc ttacacaccc gtgcctgatc ctacaccaat gatgcctcca
     5221 gtgggcgtgc aggccagcat cctgtctcac gataccatca gaatcacctg ggccgacaac
     5281 agcctgccta agcaccagaa gatcaccgac agccggtact ataccgtgcg ctggaaaaca
     5341 aacatccccg ccaacaccaa gtacaagaac gccaacgcca ccacactgag ctacctggtt
     5401 acaggcctga agcctaacac actgtatgag ttcagcgtga tggtcaccaa gggcagaaga
     5461 tccagcacct ggtctatgac agcccacggc accacattcg agctggtgcc tacaagccct
     5521 ccaaaggatg tgaccgtggt gtccaaagaa ggcaagccta agaccatcat cgtgaattgg
     5581 cagccaccta gcgaggccaa cggcaagatc acaggctata tcatctacta cagcaccgac
     5641 gtgaacgctg agatccacga ctgggtcatc gagcctgtcg tgggcaacag actgacccac
     5701 cagatccaag agctgaccct ggataccccg tactacttta agattcaggc ccggaactcc
     5761 aaaggcatgg gccctatgtc tgaggccgtg cagtttagaa cccctaaggc cgacagctcc
     5821 gacaagatgc ctaatgatca ggccagcggc agcggcggaa aaggatcaag acttcctgat
     5881 ctgggctccg attacaagcc tcctatgagc ggcagcaata gccctcacgg atctcccacc
     5941 tctccactgg acagcaacat gcttggcgcg ccgggcggcg acaagaccca cacctgcccc
     6001 ccctgcccag ctccagaact gctgggaggg ccttccgtgt tcctgttccc ccccaagccc
     6061 aaggacaccc tgatgatcag caggaccccc gaggtgacct gcgtggtggt ggccgtgtcc
     6121 cacgaggacc cagaggtgaa gttcaactgg tacgtggacg gcgtggaggt gcacaacgcc
     6181 aagaccaagc ccagagagga gcagtacaac agcacctaca gggtggtgtc cgtgctgacc
     6241 gtgctgcacc aggactggct gaacggcaaa gaatacaagt gcaaagtctc caacaaggcc
     6301 ctggctgccc caatcgaaaa gacaatcagc aaggccaagg gccagccacg ggagccccag
     6361 gtgtacaccc tgccccccag ccgggaggag atgaccaaga accaggtgtc cctgacctgt
     6421 ctggtgaagg gcttctaccc cagcgatatc gccgtggagt gggagagcaa cggccagccc
     6481 gagaacaact acaagaccac ccccccagtg ctggacagcg acggcagctt cttcctgtac
     6541 agcaagctga ccgtggacaa gtccaggtgg cagcagggca acgtgttcag ctgcagcgtg
     6601 atgcacgagg ccctgcacaa ccactacacc cagaagtccc tgagcctgag ccccggcggc
     6661 agcggtctta atgatatttt cgaggctcag aagattgagt ggcatgaaca ccaccaccat
     6721 caccactaag gccggcctgt agtcactggc acaacgcgtt acccggtaag ccaatcgggt
     6781 atacacggtc gtcatactgc agacagggtt cttctacttt gcaagatagt cttgagtagt
     6841 aaaataaata gatagagaaa atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     6901 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     6961 taataaaatg aggaaattgc atcacaacac tcaaccctat ctcggtctat tcttttgatt
     7021 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     7081 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     7141 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     7201 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7261 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     7321 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     7381 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     7441 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     7501 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat gaccgagtac
     7561 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     7621 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     7681 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     7741 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     7801 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     7861 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     7921 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     7981 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacatccgcg
     8041 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     8101 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaca cgtgctacga
     8161 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     8221 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     8281 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8341 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8401 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc attaccaatg
     8461 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8521 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagcgctgc
     8581 gatgataccg cgagaaccac gctcaccggc tccggattta tcagcaataa accagccagc
     8641 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8701 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8761 catcgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8821 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8881 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8941 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9001 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9061 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9121 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9181 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9241 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9301 ttgaatactc atattcttcc tt
//
