LOCUS       sequence-303175-        9535 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9535)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-NRCAM-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..9535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             6877..6921
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             6922..6939
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7079..7096)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7085..7196
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7314..7334)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7319..7648
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7499..7634
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7561..7580
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7696..7743
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7762..8361
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7762..7781)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8258..8278
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8491..8612
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8528..8547)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8582..8601
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8665..9456,9457..9525))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9288..9307
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9526..9535,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccca aatgatttct
     2761 gccctggaag tgcctctgga ccccaagctg ctggaagatc tggtgcagcc tcctaccatc
     2821 acacagcaga gccccaagga ctacatcatc gaccccagag aaaacatcgt gatccagtgc
     2881 gaggccaagg gcaagcctcc tccaagcttt agctggacca gaaacggcac ccacttcgat
     2941 attgacaagg accctctggt caccatgaag cccggaaccg gcacactgat catcaacatc
     3001 atgagcgagg gcaaagccga gacatacgag ggcgtgtacc agtgcaccgc cagaaatgaa
     3061 cggggagccg ccgtgtccaa caacatcgtc gttagaccca gcagatcccc tctgtggacc
     3121 aaagagaagc tggaacccat cactctgcag tccggccagt ctctggtgct gccttgtaga
     3181 cctcctatcg gcctgcctcc tcctatcatc ttctggatgg acaacagctt ccagcggctg
     3241 ccccagagcg aaagagttag ccaaggcctg aacggcgacc tgtacttcag caacgtgctg
     3301 cccgaggaca ccagagagga ttacatctgc tacgcccggt tcaatcacac ccagaccatc
     3361 cagcagaaac agcccatcag cgtgaaagtg atcagcgtgg acgagctgaa cgacacaatc
     3421 gccgccaacc tgagcgacac cgagttttac ggcgccaaga gcagcagaga gcggcctcca
     3481 acatttctga cccctgaggg caacgccagc aacaaagagg aactgcgggg aaacgtgctg
     3541 agcctggaat gtattgccga gggcctgcct acaccaatta tctactgggc caaagaggac
     3601 ggcatgctgc ccaagaaccg gaccgtgtac aagaacttcg agaaaaccct gcagatcatc
     3661 cacgtgtccg aggccgacag cggcaactat cagtgtatcg ccaagaatgc cctgggcgcc
     3721 atccaccaca caatcagcgt tagagtgaag gccgctcctt attggatcac agcccctcag
     3781 aacctggtgc tgtctcctgg cgaagatggc accctgatct gtagagccaa cggcaacccc
     3841 aagcctcgga tcagctggct gacaaatggc gtgcccatcg agatcgcccc tgacgaccct
     3901 agcagaaaga tcgacggcga caccatcatc ttttccaacg tgcaagagcg gagcagcgcc
     3961 gtctaccagt gtaatgccag caacgagtac ggctacctgc tggccaacgc cttcgtgaat
     4021 gtgctggccg agcctcctag aatcctgaca ccagccaaca cactgtacca agtgatcgcc
     4081 aacagacccg ctctgctgga ctgcgccttt tttggctctc ccctgcctac catcgagtgg
     4141 ttcaaaggcg ccaagggatc tgccctgcac gaggacatct atgtgctgca cgagaacgga
     4201 accctggaaa tccccgtggc tcagaaggat agcaccggca cctatacctg tgtggccaga
     4261 aacaagctcg gcatggccaa gaacgaagtg cacctcgaga tcaaggaccc cacctggatc
     4321 gtgaagcagc ctgagtatgc tgtggtgcag aggggctcca tggtgtcctt cgagtgcaaa
     4381 gtgaagcacg atcacaccct gagcctgacc gtgctgtggc tgaaggacaa tagagagctg
     4441 cccagcgacg agcggttcac cgtggataag gatcatctgg tggtggccga tgtgtccgac
     4501 gatgactctg gcacctacac atgcgtggca aacaccacac tggacagcgt gtcagcttcc
     4561 gccgtgctgt ctgtggttgc ccctacacct acaccagctc cagtgtacga cgtgcccaat
     4621 cctcctttcg acctggaact gaccgaccag ctggacaaga gcgtgcagct gtcttggaca
     4681 cccggcgacg acaacaacag ccccatcacc aagttcatca tcgagtacga ggacgccatg
     4741 cacaagcctg gactgtggca ccatcagacc gaagtgtctg gcacccagac aactgcccag
     4801 ctgaagctga gcccctacgt gaactactcc ttccgcgtga tggccgtgaa cagcatcggc
     4861 aagtctctgc cttctgaggc cagcgagcag tacctgacaa aggcctccga gcctgacaag
     4921 aaccctacag ccgttgaagg cctgggcagc gagcctgata atctggtcat cacctggaag
     4981 cccctgaatg gcttcgagag caatggccct ggcctgcagt ataaggtttc ctggcgacag
     5041 aaggatggcg acgacgagtg gacatctgtg gtggtcgcta acgtgtccaa gtatatcgtg
     5101 tccggcacac ccaccttcgt gccctacctg attaaggtgc aggccctgaa cgatatgggc
     5161 ttcgctcctg aacctgccgt ggtcatggga cacagcggag aggatctgcc tatggtggct
     5221 cctggcaatg tgcgcgtgaa cgtggtcaat agcacactgg ccgaagtgca ttgggacccc
     5281 gtgcctctga agtccatcag aggccatctc cagggctaca gaatctacta ctggaaaacc
     5341 cagagcagct ccaagcggaa cagacggcac atcgagaaga agatcctgac atttcagggc
     5401 agcaagaccc acggaatgct gcctggcctg gaacctttca gccactacac cctgaatgtg
     5461 cgggtcgtga acggcaaagg cgagggacct gcttctcccg acagagtgtt caatacccca
     5521 gagggcgtgc caagcgctcc ttcctctctg aaaatcgtga accctactct ggacagcctg
     5581 acactggaat gggaccctcc atctcacccc aacggcatcc tgaccgagta cacactgaag
     5641 taccagccta tcaacagcac ccacgagctg ggccctctgg tggatctgaa gatccctgcc
     5701 aacaagacca ggtggaccct gaagaatctg aacttcagca cccggtacaa gttctacttc
     5761 tacgcccaga caagcgccgg cagcggaagc cagattactg aagaggccgt gaccacagtg
     5821 gacgaggccg gaattctgcc tcctgatgtt ggagccggca aagtgcaggc tgtgaacccc
     5881 agaatcagca acctgacagc cgccgctgcc gaaacctacg ccaatatcag ctgggagtat
     5941 gagggccccg agcacgtgaa cttctacgtg gaatatggcg tggccggcag caaagaagag
     6001 tggcggaaag aaattgtgaa cggctctcgg agcttcttcg gcctgaaggg actgatgcct
     6061 ggcaccgcct acaaagttcg agtgggagcc gtgggcgata gcggctttgt gtctagcgag
     6121 gacgtgttcg agacaggccc tgccatggct tccagacagg tggacattgc cacacaaggc
     6181 gcgccgggcg gcgacaagac ccacacctgc cccccctgcc cagctccaga actgctggga
     6241 gggccttccg tgttcctgtt cccccccaag cccaaggaca ccctgatgat cagcaggacc
     6301 cccgaggtga cctgcgtggt ggtggccgtg tcccacgagg acccagaggt gaagttcaac
     6361 tggtacgtgg acggcgtgga ggtgcacaac gccaagacca agcccagaga ggagcagtac
     6421 aacagcacct acagggtggt gtccgtgctg accgtgctgc accaggactg gctgaacggc
     6481 aaagaataca agtgcaaagt ctccaacaag gccctggctg ccccaatcga aaagacaatc
     6541 agcaaggcca agggccagcc acgggagccc caggtgtaca ccctgccccc cagccgggag
     6601 gagatgacca agaaccaggt gtccctgacc tgtctggtga agggcttcta ccccagcgat
     6661 atcgccgtgg agtgggagag caacggccag cccgagaaca actacaagac caccccccca
     6721 gtgctggaca gcgacggcag cttcttcctg tacagcaagc tgaccgtgga caagtccagg
     6781 tggcagcagg gcaacgtgtt cagctgcagc gtgatgcacg aggccctgca caaccactac
     6841 acccagaagt ccctgagcct gagccccggc ggcagcggtc ttaatgatat tttcgaggct
     6901 cagaagattg agtggcatga acaccaccac catcaccact aaggccggcc tgtagtcact
     6961 ggcacaacgc gttacccggt aagccaatcg ggtatacacg gtcgtcatac tgcagacagg
     7021 gttcttctac tttgcaagat agtcttgagt agtaaaataa atagatagag aaaatcagcc
     7081 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     7141 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcacaa
     7201 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     7261 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     7321 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7381 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     7441 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     7501 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7561 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     7621 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     7681 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     7741 gacaaggtga ggaactaaac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     7801 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     7861 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     7921 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     7981 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     8041 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     8101 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     8161 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     8221 gccggggtgc ccgccttcct ggagacatcc gcgccccgca acctcccctt ctacgagcgg
     8281 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     8341 acccgcaagc ccggtgcctg acacgtgcta cgagatttcg attccaccgc cgccttctat
     8401 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     8461 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     8521 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8581 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     8641 tagagcttgg cgtaatcatg gtcattacca atgcttaatc agtgaggcac ctatctcagc
     8701 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8761 acgggagggc ttaccatctg gccccagcgc tgcgatgata ccgcgagaac cacgctcacc
     8821 ggctccggat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8881 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8941 ttcgccagtt aatagtttgc gcaacgttgt tgccatcgct acaggcatcg tggtgtcacg
     9001 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9061 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9121 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9181 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9241 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9301 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9361 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9421 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9481 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatattct tcctt
//
